Polysaccharides From Abrus cantoniensis Hance Modulate Intestinal Microflora and Improve Intestinal Mucosal Barrier and Liver Oxidative Damage Induced by Heat Stress

Abstract

The protective effects of polysaccharides from Abrus cantoniensis Hance (ACP) on antioxidant capacity, immune function, the hypothalamus-pituitary-adrenal (HPA) axis balance, the intestinal mucosal barrier, and intestinal microflora in heat stress (HS)-induced heat-injured chickens are rarely reported. The purpose of this study was to investigate the protective effects of ACP on HS-injured chickens by enhancing antioxidant capacity and immune function, repairing the intestinal mucosal barrier, and regulating intestinal microflora. A total of 120 native roosters in Guangxi were randomly divided into 5 groups to evaluate the protective effect of ACP on chickens injured by HS (33 ± 2°C). The results showed that ACP increased the body weight and the immune organ index of heat-injured chickens, regulated the oxidative stress kinase secretion, and restored the antioxidant level of heat-injured birds. ACP significantly inhibited the secretion of corticotropin releasing hormone (CRH), adrenocorticotropic hormone (ACTH), and corticosterone (COR) and reversed the disorder of hormone levels caused by HS. ACP significantly regulated the secretion levels of immune cytokines and restored the immune function of the body. ACP significantly improved the intestinal morphology and increased the expression levels of tight junction proteins, which had a positive effect on protecting intestinal health. The results of high-throughput sequencing of the 16S rRNA gene showed that HS led to an increase in the abundance of harmful bacteria and an abnormal increase in the abundance of intestinal microflora and that ACP restored the HS-induced intestinal microflora imbalance. In conclusion, this study provides a scientific basis for ACP as an antioxidant activity enhancer to reduce liver injury, regulate intestinal microflora, and protect intestinal mucosal damage in chickens.

Comparison study of protective effects of porcine bile acids and sheep bile acids against heat stress in chickens

“…The ⊍ diversity study showed that the ACE and Chao1 indices of the diet supplemented with BAs were higher than those of the HS group, which could be because HS impacts the integrity of the intestine and promotes the colonization of harmful bacteria in the intestine. 40 Accordingly, based on the principal component analysis curve of the cecum, the HS group exhibited a distinct cluster that was clearly separated from those in the Mock group. Based on the Wayne diagram, the number of OTUs in the HS group decreased, and the influence of HS was significant.…”

“…Intestinal flora disorder can induce inflammation and immune response, 39 which can in turn induce intestinal flora. The α diversity study showed that the ACE and Chao1 indices of the diet supplemented with BAs were higher than those of the HS group, which could be because HS impacts the integrity of the intestine and promotes the colonization of harmful bacteria in the intestine 40 . Accordingly, based on the principal component analysis curve of the cecum, the HS group exhibited a distinct cluster that was clearly separated from those in the Mock group.…”

The impact of dietary supplementation of polysaccharide derived from Polygonatum sibiricum on growth, antioxidant capacity, meat quality, digestive physiology, and gut microbiota in broiler chickens

“…The utilization of plant-derived polysaccharides for enhancing the productivity and general health of broilers has gained traction due to their varied biological activities, including hypoglycemic benefits, spanning improvements in immunity, antioxidant capacity, antiviral, antitumor, and anti-inflammatory properties ( Wang et al, 2022b ; Yang et al, 2023 ). The bioactive potential of polysaccharides derived from Yingshan yunwu, Radix rehmanniae praeparata, Lycium barbarum, Astragalus membranaceus, Camellia oleifera, and Ficus carica has been investigated ( Liu et al, 2021b ; Shu et al, 2021 ; Yang et al, 2023 ).…”

“…Integrating TCM in poultry production aligns with eco-friendly practices and disease prevention. Recent research underscores the advantageous effects of plant-derived bioactives, particularly polysaccharides, in enhancing poultry growth, meat quality, and carcass characteristics ( Wang et al, 2022b ; Yang et al, 2023 ). Extensive research focused on polysaccharides commonly found in TCM plants and their extracts, which are rich in polysaccharides, have gained attention as AGP alternatives because of their multifunctionality, low toxicity, and limited adverse effects ( Shan et al, 2019 ; Abdel-Moneim et al, 2020b ; Sun et al, 2022 ).…”

Simultaneous determination of abrine, hypaphorine, schaftoside and soyasaponin Bb in rat plasma by UPLC–MS/MS and its application to a pharmacokinetic study after oral administration of Abrus cantoniensis Hance extract

“…As an edible vegetable and medicinal plant, Abrus cantoniensis Hance is a woody climbing shrub found in the southern regions of China, such as Guangdong, Guangxi, Fujian and Hainan provinces (Liu et al, 2022). It has the effects of eliminating dampness and relieving yellowing, clearing away heat and detoxification, soothing the liver and relieving pain (Wang, Sun, et al, 2022; Yang et al, 2015). Recently many studies have reported that A. cantoniensis Hance possesss anti‐hepatitis (Wong et al, 2005; Yao et al, 2020), anti‐tumor and immunomodulatory capabilities (Wu et al, 2016) and free radical scavenging and reducing ability (Yang et al, 2014, 2015).…”