Polysaccharides from Angelica sinensis alleviate neuronal cell injury caused by oxidative stress

Abstract

Angelica sinensis has antioxidative and neuroprotective effects. In the present study, we aimed to determine the neuroprotective effect of polysaccharides isolated from Angelica sinensis. In a preliminary experiment, Angelica sinensis polysaccharides not only protected PC12 neuronal cells from H2O2-induced cytotoxicity, but also reduced apoptosis and intracellular reactive oxygen species levels, and increased the mitochondrial membrane potential induced by H2O2 treatment. In a rat model of local cerebral ischemia, we further demonstrated that Angelica sinensis polysaccharides enhanced the antioxidant activity in cerebral cortical neurons, increased the number of microvessels, and improved blood flow after ischemia. Our findings highlight the protective role of polysaccharides isolated from Angelica sinensis against nerve cell injury and impairment caused by oxidative stress.

Polysaccharide from Angelica sinensis protects H9c2 cells against oxidative injury and endoplasmic reticulum stress by activating the ATF6 pathway

“… 27 ASP exerts neuroprotective effects by limiting cerebral ischaemia/reperfusion injury, 28 increasing microvessel number, and improving blood flow after cerebral ischaemia. 29 The antioxidant activity of ASP has been tested in in vivo and in vitro experimental models. Zhuang et al.…”

“…showed that ASP attenuated an H 2 O 2 -induced increase in apoptosis and ROS levels in PC12 neuronal cells, and enhanced the activity of antioxidant enzymes in cortical tissues of rats with focal cerebral ischaemia. 29 Additionally, ASP treatment reduced the senescence of endothelial progenitor cells by relieving OS. 31 However, the effect of ASP on oxidative injury in cardiomyocytes and mechanisms underlying this effect are unclear.…”

“…These observations are consistent with the results of previous studies in other cell types showing the anti-apoptotic effect of ASP. 29 , 30 …”

Bioactive Peptides from Angelica sinensis Protein Hydrolyzate Delay Senescence in Caenorhabditis elegans through Antioxidant Activities

“…It has also been used as health food for women's care in Europe and America [ 14 ]. Recent studies have shown that polysaccharides, among other products, from A. sinensis have antioxidant and neuroprotective functions [ 15 ], but similar activities have not been addressed on peptides from A. sinensis . Using the nematode Caenorhabditis elegans , a powerful model organism for aging and neurobiology studies, we investigated the antioxidant and anti-aging activities of peptide preparations derived from A. sinensis roots, including their effect on oxidative survival, ROS levels, antioxidant enzyme activities, lipid peroxidation product content, age pigments content, and lifespan.…”

Food-Derived Antioxidant Polysaccharides and Their Pharmacological Potential in Neurodegenerative Diseases

“…In particular, polysaccharides isolated from functional and medicinal foods as well as from common foods have drawn great attention in antioxidant studies. Here, we attempt to summarize recent studies of antioxidant polysaccharides from food resources, including vegetables, fruits, cereals, beans, mushrooms, tea, milk products and meat ( Table 1 ) [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 ].…”