Polysaccharides from Dendrobium officinale inhibit bleomycin-induced pulmonary fibrosis via the TGFβ1-Smad2/3 axis

Chen, Jianhui; Lu, Junhui; Wang, Baolan; Zhang, Xiaolei; Huang, Qiuyang; Yuan, Jian; Hao, Hairong; Chen, Xing; Zhi, Junchao; Zhao, Liang; Chu, Haiqing

doi:10.1016/j.ijbiomac.2018.07.056

Cited by 30 publications

(15 citation statements)

References 29 publications

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“…Presently, the pharmacotherapy agent options are pirfenidone and nintedanib, which only received conditional suggestions in view of clinical effects, costs and safety profiles (Zhang et al, 2018a;Chen et al, 2018;Saito et al, 2019). Cortical hormone is also employed to treat PF, and prednisone acetate (PA) is the common selected drug for positive control studies in bleomycin-induced PF model (Ji et al, 2016;Tao et al, 2017;Zhang et al, 2018b;Chen et al, 2018). Traditional herbal medicines, with their efficacy and advantages have being investigated over years, have been considered to be potential candidates for treating IPF (Yao and Jiang, 2003).…”

Section: Introductionmentioning

confidence: 99%

Sodium Houttuyfonate Inhibits Bleomycin Induced Pulmonary Fibrosis in Mice

et al. 2021

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Pulmonary fibrosis (PF) could severely disrupt the normal lung architecture and function with fatal consequences. Currently, there is no effective treatment for PF or idiopathic pulmonary fibrosis (IPF). The aim of this study was to investigate the effects of Sodium Houttuyfonate (SH) on bleomycin (BLM) induced PF mice model. Our results indicated that SH could attenuate BLM induced lung injury by reducing the inflammation, fibrogenesis and lung/body weight ratio. The proposed mechanisms for the protective effects of SH include: 1) improvement of pulmonary function in BLM mice, for instance, it can elevate the vital capacity (VC), increase the forced expiratory flow at 50% of forced vital capacity (FEF50) and improve other pulmonary function indices; 2) inhibition of collagen formation in BLM mice; 3) attenuation of the elevation of inflammatory cytokines, such as interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α), which are triggered by BLM administration; 4) reduction of the mRNA level and protein production of transforming growth factor-β1 (TGF-β1) in BLM mice. Furthermore, it was found that the protective effects of SH against BLM induced PF in mice was comparable to that of prednisone acetate (PA) tablets, a widely used drug for immunological diseases. Although Houttuynia Cordata Thunb has been widely used in China for lung infection and inflammation, the mechanism has not yet been fully elucidated. Our study provides the evidence that SH is an effective compound against pulmonary injury, irritation and fibrogenesis.

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Section: Introductionmentioning

confidence: 99%

Sodium Houttuyfonate Inhibits Bleomycin Induced Pulmonary Fibrosis in Mice

et al. 2021

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“…Dendrobium officinale, also known as Tiepi Shihu in Chinese, is a well-known herb in Traditional Chinese Medicine and has been used for more than 2,000 years. Furthermore, this important species has been listed as a health food by the National Health Commission of the People's Republic of China (He et al 2017;Chen et al 2018;Zhao et al 2019). In the current study, we used a comprehensive approach to examine differences in composition and gene regulation of polysaccharides and flavonoids between DOPs and Mat; in addition, we identified several genes related to the biosynthesis of these compounds using transcriptome analysis.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of transcriptome and metabolome uncovers the metabolic differences between Dendrobium officinale protocorms and mature stems

Mai

Yang

et al. 2020

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Dendrobium officinale caulis is the dried mature stems (Mat) of D. officinale Kimura et Migo. D. officinale protocorms (DOPs) are the conical embryonic tissues formed after seed germination. Previous studies suggest that these different plant parts possess similar pharmacological activities. However, the metabolic profiles and gene expression across different growth stages of D. officinale remain unknown. In this study, metabolome analysis based on GC and LC/MS/MS was carried out to assess the metabolic profile of both DOPs and Mat. Transcriptome analysis was used to reveal internal relationships between metabolites and the differing gene regulation. The results indicated that although DOPs and Mat had a similar chemical composition, they exhibited different patterns of content. GO classification and KEGG clustering of the differentially expressed genes (DEGs) between DOPs and Mat demonstrated that gene enrichment was mainly related to flavonoid biosynthetic and metabolic processes, carbohydrate metabolic processes, and polysaccharide catabolic processes. An alteration in gene expression related to specific metabolic pathways offered a probable explanation for the observed differences in metabolites between DOPs and Mat. This work provides a foundation for subsequent studies regarding the biosynthetic pathways of related metabolites from D. officinalis.

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“…officinale is a traditional but endangered Chinese orchid. As an important component of D. officinale, polysaccharide has significant positive effects such as antioxidation, acute colitis, pulmonary fibrosis, and apoptosis (Zhao et al, 2017;Chen et al, 2018a;Liang et al, 2018bLiang et al, , 2019. In order to improve the polysaccharide yield of D. officinale, agricultural and polysaccharide processing approaches such as fertilizer application and optimized polysaccharide extraction and dehydration methods have been applied (He et al, 2018;Qian et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Among these substances, polysaccharides, which are the most important medicinal substances of D. officinale, attract many researchers' attention. Recently, polysaccharides have been proven to have positive effects on the improvement of antioxidant activity and alleviation of colitis, pulmonary fibrosis, and apoptosis (Huang et al, 2016;Zhang et al, 2017a;Chen et al, 2018a;Liang et al, 2018a). What is more, the bioactivities, composition, structure, and physicochemical properties of polysaccharides from Dendrobium have been well defined (Wei et al, 2016;Zhang et al, 2016;Ma et al, 2018), and a number of critical genes related to polysaccharide synthesis have been characterized as well, such as uridine diphosphate glucose pyrophosphorylases (UGPases), sucrose-phosphate synthases (SPSs), sucrose synthases (SuSys), and glycosyltransferases (GTs) including cellulose synthase like A (CSLAs) genes (He et al, 2015;Yan et al, 2015;Shen et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of DoUGP Enhanced Biomass and Stress Tolerance by Promoting Polysaccharide Accumulation in Dendrobium officinale

et al. 2020

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Uridine diphosphate glucose pyrophosphorylase (UDP-glucose pyrophosphorylase, UGPase), as one of the key enzymes in polysaccharide synthesis, plays important roles in the growth and development of plants. In this study, the DoUGP gene of Dendrobium officinale was overexpressed. The expression of DoUGP and genes playing roles in the same and other saccharide synthesis pathways was determined, and the total soluble polysaccharide was also tested in wild-type and transgenic seedlings. We also performed freezing and osmotic stress treatments to determine whether overexpression of DoUGP could influence stress resistance in transgenic seedlings. Results showed that mRNA expression levels of DoUGP and its metabolic upstream and downstream genes in the transgenic seedlings were increased compared to the expression of these genes in wild-type seedlings. Additionally, most CSLA genes involved in the biosynthesis of mannan polysaccharides were significantly upregulated. The total polysaccharide and mannose content of transgenic seedlings were increased compared to the content of wild type, and enhanced stress tolerance was found in the overexpressed seedlings compared to the wild type.

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Polysaccharides from Dendrobium officinale inhibit bleomycin-induced pulmonary fibrosis via the TGFβ1-Smad2/3 axis

Cited by 30 publications

References 29 publications

Sodium Houttuyfonate Inhibits Bleomycin Induced Pulmonary Fibrosis in Mice

Sodium Houttuyfonate Inhibits Bleomycin Induced Pulmonary Fibrosis in Mice

Comparative analysis of transcriptome and metabolome uncovers the metabolic differences between Dendrobium officinale protocorms and mature stems

Overexpression of DoUGP Enhanced Biomass and Stress Tolerance by Promoting Polysaccharide Accumulation in Dendrobium officinale

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