Polysaccharides from<i>Hibiscus sabdariffa</i>Flowers Stimulate Proliferation and Differentiation of Human Keratinocytes

Brunold, Christian; Deters, Alexandra; Knoepfel-Sidler, F.; Hafner, Johanna; Müller, Benedikt; Hensel, Andreas

doi:10.1055/s-2004-818952

Cited by 30 publications

(7 citation statements)

References 10 publications

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“…Since arabinan and arabinogalactan chains covalently linked to RG-I structures have been associated with strong biological activities [3] and especially keratinocyte proliferation and differentiation [6,9,10,11], we wanted to investigate the structural characteristics of three extracts - an arabinan and two AG-II type RG-I polysaccharides - with the aim to characterize the variability of their properties and then to identify the structural origin of their bioactivity. Mr 27,000 heat shock protein (HSP-27) is a member of the small heat shock protein family.…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, increased degrees of polymerization as well as extensive branching and ramification seem to enhance their biological activities [8]. Rhamnogalacturonan from Hibiscus sabdariffa was shown to stimulate the proliferation and differentiation of human keratinocytes [9]. Deters and coworkers recently suggested that terminal arabinose residues of type I RG arabinogalactan side chains [10], as well as rhamnose content [11], could justify the observed bio-activity of pectins.…”

Section: Introductionmentioning

confidence: 99%

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Structural Patterns of Rhamnogalacturonans Modulating Hsp-27 Expression in Cultured Human Keratinocytes

et al. 2008

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Polysaccharide extracts were obtained from chestnut bran (Castanea sativa), grape marc (Vitis vinifera) and apple marc (Malus spp.) and fractionated by size exclusion chromatography after endopolygalacturonase degradation. Compositional and linkage analyses by GC and GC-MS showed the characteristic rhamnogalacturonan structure with specific arabinan (apple marc) and type II arabinogalactan (chestnut bran, grape marc) side chains. Type II arabinogalactan rhamnogalacturonan from chestnut bran significantly stimulated the in vitro differentiation of human keratinocytes, giving evidence of a tight structure-function relationship. This molecule comprises short and ramified 3- and 3,6-β-d-galactan and 5- and 3,5-α-l-arabinan side chains, but also contains significant amounts of t‑Xyl and 4‑Xyl with a characteristic 2:1 ratio. Enzymatic hydrolysis of this polysaccharide produced fragments of lower molecular weight with unchanged xylose content which conserved the same ability to stimulate human keratinocyte differentiation. It could be then speculated that dimeric xylosyl-xylose and/or longer oligomeric xylose side chains attached to a galacturonan and closely associated to hairy rhamno-galacturonan domains are essential patterns that could determine the biological activity of pectins.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Structural Patterns of Rhamnogalacturonans Modulating Hsp-27 Expression in Cultured Human Keratinocytes

et al. 2008

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“…The Roselle plant ( Hibiscus sabdariffa ) is a shrub with the characteristic five petals, funnel shaped flower belonging to the malvaceae family ( Brunold et al . 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Preservation of Lycopersicum esculentum (Tomatoes) with Extracts of Annona muricata (Soursop) and Hibiscus sabdariffa (Roselle Plant)

Banjo¹,

Oluwole²,

Nzei³

2022

TLSR

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Tomatoes are very important vegetable crops in the world but with a perishable nature. Due to its highly perishable nature, various methods have been investigated to increase its shelf life while still preserving its qualities. Therefore, this study investigated the potentials of the parts of Annona muricata and the calyces of Hibiscus sabdariffa in the preservation of tomato fruits. Tomato fruits were washed and treated with powdered, aqueous and ethanolic extracts of the leaves, seeds, bark of the Annona muricata and calyces of Hibiscus sabdariffa at different concentrations of 3%, 5%, 6%, 9% and 12% w/v. The tomato fruits were placed in well-aerated baskets for a period of 35 days during which organoleptic and microbial analysis were carried out. The different treatments with Annona muricata and Hibiscus sabdariffa had significant effects on the preservation of the tomato fruits at p < 0.05. The leaves of Annona muricata proved most effective preserving up to 50% of the tomatoes after the monitoring period. Moreover, 6% (w/v) of the aqueous extract of the leaves of Annona muricata resulted in a preservation rate of 75% of the tomato fruits. The spoilage microorganisms isolated from the tomato fruits are Candida krusei, Candida sp., Bacillus subtilis and Bacillus sp. The results of this research carried out shows that the extracts of Annona muricata and Hibiscus sabdariffa had significant preservative activities on the tomato fruits (p < 0.05), thus minimising wastes and economic loss to the farmers and country in general.

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“…18 In a separate study, arabinose, galactose, glucose, rhamnose, galacturonic acid, glucuronic acid, mannose, and xylose were identified in calyces by ion-exchange chromatography. 19 Twelve phenolic compounds, glucose, and fructose, along with other nutrients, were identified by one study on flowers of H. sabdarif fa, and a second study reported 18 phenolic compounds from flowers. 20,21 Hibiscus seeds contain mainly volatile compounds such as unsaturated hydrocarbons, alcohols, and aldehydes.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Recently, ultraperformance liquid chromatography coupled to electrospray ionization quadrupole-time-of-flight mass spectrometry (UPLC-ESI-Q-TOF-MS n ) was used to identify 33 phytochemicals, which included hydroxycinnamic acids, organic acids, anthocyanins, and flavonoids from H. sabdariffa calyces . In a separate study, arabinose, galactose, glucose, rhamnose, galacturonic acid, glucuronic acid, mannose, and xylose were identified in calyces by ion-exchange chromatography . Twelve phenolic compounds, glucose, and fructose, along with other nutrients, were identified by one study on flowers of H. sabdariffa , and a second study reported 18 phenolic compounds from flowers. , Hibiscus seeds contain mainly volatile compounds such as unsaturated hydrocarbons, alcohols, and aldehydes .…”

Section: Introductionmentioning

confidence: 99%

Variation of Phytochemicals in Leaves of Seven Accessions of Hibiscus sabdariffa Grown under Field, Green Roof, and High Tunnel Conditions

Luthria

Tareq

Marupaka

et al. 2021

ACS Food Sci. Technol.

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The phytochemical composition in the leaves of seven accessions of Hibiscus sabdariffa L. grown under field, green roof, and high tunnel conditions was investigated in the present study. Principle component analysis of freeze-dried leaves using near-infrared (NIR) spectral fingerprinting revealed differences between accessions and the three growing conditions. Under all conditions, NIR fingerprint data of accession PI 286316 showed variation from the other six accessions (PI 256041, PI 267778, PI 273388, PI 275414, Thai red, and UDC green). A detailed investigation of the phytochemical composition of the accessions by high-performance liquid chromatography coupled with diode array and high-resolution mass spectrometry showed the presence of 16 bioactive phenolics. In six accessions, ten phenolics were identified as compared to eight phenolics in accession 286316, six of which from the latter were unique and not previously reported in H. sabdariffa leaves. Only two phenolics, namely neochlorogenic acid and chlorogenic acid, were common in all seven accessions. Total phenolics in samples from the green roof were 12% and 16% higher than those from the field and high tunnel, respectively. Ten free soluble amino acids and three soluble sugars were identified in all accessions. However, total amino acids and sugars in leaves from the field were between 24 and 42% higher than leaves from the green roof and high tunnel. This study shows that H. sabdariffa leaves provide a good source for bioactive phenolics, free amino acids, and sugars, hence, it can be a nutritious addition to meals.

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Polysaccharides fromHibiscus sabdariffaFlowers Stimulate Proliferation and Differentiation of Human Keratinocytes

Cited by 30 publications

References 10 publications

Structural Patterns of Rhamnogalacturonans Modulating Hsp-27 Expression in Cultured Human Keratinocytes

Structural Patterns of Rhamnogalacturonans Modulating Hsp-27 Expression in Cultured Human Keratinocytes

Preservation of Lycopersicum esculentum (Tomatoes) with Extracts of Annona muricata (Soursop) and Hibiscus sabdariffa (Roselle Plant)

Variation of Phytochemicals in Leaves of Seven Accessions of Hibiscus sabdariffa Grown under Field, Green Roof, and High Tunnel Conditions

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