Polysaccharides from <i>Laminaria japonica</i>: an insight into the current research on structural features and biological properties

Luan, Fei; Zou, Junbo; Rao, Zhili; Ji, Yafei; Lei, Ziqin; Peng, Li‐Xia; Yang, Yan; He, Xirui; Zeng, Nan

doi:10.1039/d1fo00311a

Cited by 36 publications

(21 citation statements)

References 111 publications

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“…For example, the fucoidan fraction of S. japonica showed the optimal anti-inflammatory activity in concentrations of 50-200 μg/mL [49] and 50-400 μg/mL [50], respectively. Besides, some studies reviewed the biological activities of fucoidan, and considered that the content of fucose, sulfate groups, molecular weight, and position of the glycosidic linkage would affect the biological activities of fucoidan [32,52,53]. Ye et al (2020) indicated that the purified fucoidan, from 50 to 200 μg/mL (246.4 KDa), with high sulfate group (36.94%) and rich in fucose (24.15%), had better anti-inflammatory activity [49].…”

Section: Cell Viability and No Production To Ljnfmentioning

confidence: 99%

Molecular Mechanism of Anti-Inflammatory Activities of a Novel Sulfated Galactofucan from Saccharina japonica

Chen

et al. 2021

Marine Drugs

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Seaweed of Saccharina japonica is the most abundantly cultured brown seaweed in the world, and has been consumed in the food industry due to its nutrition and the unique properties of its polysaccharides. In this study, fucoidan (LJNF3), purified from S. japonica, was found to be a novel sulfated galactofucan, with the monosaccharide of only fucose and galactose in a ratio of 79.22:20.78, and with an 11.36% content of sulfate groups. NMR spectroscopy showed that LJNF3 consists of (1→3)-α-l-fucopyranosyl-4-SO3 residues and (1→6)-β-d-galactopyranose units. The molecular mechanism of the anti-inflammatory effect in RAW264.7 demonstrated that LJNF3 reduced the production of nitric oxide (NO), and down-regulated the expression of MAPK (including p38, ENK and JNK) and NF-κB (including p65 and IKKα/IKKβ) signaling pathways. In a zebrafish experiment assay, LJNF3 showed a significantly protective effect, by reducing the cell death rate, inhibiting NO to 59.43%, and decreasing about 40% of reactive oxygen species. This study indicated that LJNF3, which only consisted of fucose and galactose, had the potential to be developed in the biomedical, food and cosmetic industries.

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Section: Cell Viability and No Production To Ljnfmentioning

confidence: 99%

Molecular Mechanism of Anti-Inflammatory Activities of a Novel Sulfated Galactofucan from Saccharina japonica

Chen

et al. 2021

Marine Drugs

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“…Additionally, the PSs extracted from LJ exhibit a variety of health-beneficial biofunctions, such as anti-oxidation ( 4 , 5 ), anti-inflammatory ( 6 , 7 ), lipid-lowering ( 8 , 9 ), anti-diabetes ( 10 ), and anti-obesity effects ( 11 ). Of note, the discrepant bioactivities of LJ-derived PS (LJPS) might occur owing to different isolation procedures or sources ( 7 , 12 , 13 ) and more likely due to the differentiated molecular structure of the LJPS.…”

Section: Introductionmentioning

confidence: 99%

“…Regarding the molecular structure of LJPS, most current research has focused on the analyses of monosaccharide composition, molecular weight, glycosidic linkages, and substituted groups via high-performance liquid chromatography (HPLC), near-infrared spectroscopy, gel penetration chromatography, and other methods. Moreover, with respect to the conventional indicators of molecular structure, recent studies have addressed the pivotal role of PS chain length (degree of polymerization, DP) and spatial conformation in their bioactivities ( 11 , 17 , 18 ). Similarly, many studies on inulin and fructan have shown that DP value is one of the most crucial factors affecting their biological functions ( 11 , 19 ).…”

Section: Introductionmentioning

confidence: 99%

Structural Characteristics and Immunomodulatory Effects of a Long-Chain Polysaccharide From Laminaria japonica

et al. 2022

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Polysaccharides derived from Laminaria japonica (LJPS) have shown a variety of beneficial effects on improving human health; however, the structural features and bioactivities of long-chain LJPS remain unclear. This study aimed to investigate the structural characteristics and bioactivities of a novel long-chain LJPS. Results showed that the LJPS was composed of Fuc, Rha, Ara, Gal, Glc, Xyl, Man, Fru, Rib, GalA, GluA, GlcA, and ManA, with a molar ratio of 35.71:1.48:0.28:13.16:0.55:2.97:6.92:0.58:0.41:0.14:3.16:15.84:18.79. Of these, Fuc, Gal, Man, GlcA, and ManA were the predominant components with an accumulated proportion of 93.6%. The LJPS was found to consist of seven types of the monomer residues, and the main interchain glycosidic linkages were β -D-(1 → 2), α -D-(1 → 3), (1 → 4), and (1 → 6), and the molecular mass was 5.79 × 104 g/mol. Regarding the molecular conformation, LJPS was a multi-branched, long-chain macromolecule, and appeared in a denser crosslinking network with highly branched and helix domains in the terms of morphology. Additionally, the LJPS had no toxicity to mouse macrophage cells and exhibited biphasic immuno-modulating capacity. The present findings suggested that the long-chain LJPS might be an attractive candidate as an immunopotentiating and anti-inflammatory functional food, and this study also provides a feasible approach to decipher the structural characteristics and spatial conformations of plant-derived polysaccharides.

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“…Sea tangle (Laminaria japonica Aresch), a type of edible brown algae, has long been widely consumed as a food supplement and medicinal use in East Asia including Korea. This seaweed is rich in various lipophilic components, hydrophilic fibers, and minerals, and various pharmacological effects were extensively reported in several previous studies [11][12][13][14]. Interestingly, fermented sea tangle extract (FST) with Lactobacillus brevis BJ20 content 5.56% of gamma-aminobutyric acid (GABA) that was 49.5% among total free amino acids [15].…”

Section: Introductionmentioning

confidence: 94%

Protection against Oxidative Stress-Induced Apoptosis by Fermented Sea Tangle (Laminaria japonica Aresch) in Osteoblastic MC3T3-E1 Cells through Activation of Nrf2 Signaling Pathway

Kim

Cha

Hwangbo

et al. 2021

Foods

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The purpose of the present study was to explore the efficacy of fermented extract of sea tangle (Laminaria japonica Aresch, FST) with Lactobacillus brevis on DNA damage and apoptosis in hydrogen peroxide (H2O2)-stimulated osteoblastic MC3T3-E1 cells and clarify related signaling pathways. Our results showed that exposure to FST significantly improved cell viability, inhibited apoptosis, and suppressed the generation of reactive oxygen species (ROS) in H2O2-stimulated cells. In addition, H2O2 triggered DNA damage in MC3T3-E1 cells was markedly attenuated by FST pretreatment. Moreover, H2O2-induced mitochondrial dysfunctions associated with apoptotic events, including loss of mitochondrial membrane potential (MMP), decreased Bcl-2/Bcl-2 associated x-protein (Bax) ratio, and cytosolic release of cytochrome c, were reduced in the presence of FST. FST also diminished H2O2-induced activation of caspase-3, which was associated with the ability of FST to protect the degradation of poly (ADP-ribose) polymerase. Furthermore, FST notably enhanced nuclear translocation and phosphorylation of nuclear factor erythroid 2-related factor 2 (Nrf2) in the presence of H2O2 with concomitant upregulation of heme oxygenase-1 (HO-1) expression. However, artificial blockade of this pathway by the HO-1 inhibitor, zinc protoporphyrin IX, greatly abolished the protective effect of FST against H2O2-induced MC3T3-E1 cell injury. Taken together, these results demonstrate that FST could protect MC3T3-E1 cells from H2O2-induced damage by maintaining mitochondrial function while eliminating ROS along with activation of the Nrf2/HO-1 antioxidant pathway.

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Polysaccharides from Laminaria japonica: an insight into the current research on structural features and biological properties

Abstract: Laminaria japonica, one of the most widespread seafood consumed in China and many other nations, has been traditionally utilized as an effective therapeutically active substance for treating the weight loss,...

Cited by 36 publications

References 111 publications

Molecular Mechanism of Anti-Inflammatory Activities of a Novel Sulfated Galactofucan from Saccharina japonica

Molecular Mechanism of Anti-Inflammatory Activities of a Novel Sulfated Galactofucan from Saccharina japonica

Structural Characteristics and Immunomodulatory Effects of a Long-Chain Polysaccharide From Laminaria japonica

Protection against Oxidative Stress-Induced Apoptosis by Fermented Sea Tangle (Laminaria japonica Aresch) in Osteoblastic MC3T3-E1 Cells through Activation of Nrf2 Signaling Pathway

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