Polysaccharides of Salsola passerina: Extraction, Structural Characterization and Antioxidant Activity

Abstract

The above-ground part of the Salsola passerine was found to contain ~13% (w/w) of polysaccharides extractable with water and aqueous solutions of ammonium oxalate and sodium carbonate. The fractions extracted with aqueous sodium carbonate solutions had the highest yield. The polysaccharides of majority fractions are characterized by similar monosaccharide composition; namely, galacturonic acid and arabinose residues are the principal components of their carbohydrate chains. The present study focused on the determination of antioxidant activity of the extracted polysaccharide fractions and elucidation of the structure of polysaccharides using nuclear magnetic resonance (NMR) spectroscopy. Homogalacturonan (HG), consisting of 1,4-linked residues of α-D-galactopyranosyluronic acid (GalpA), rhamnogalacturonan-I (RG-I), which contains a diglycosyl repeating unit with a strictly alternating sequence of 1,4-linked D-GalpA and 1,2-linked L-rhamnopyranose (Rhap) residues in the backbone, and arabinan, were identified as the structural units of the obtained polysaccharides. HMBC spectra showed that arabinan consisted of alternating regions formed by 3,5-substituted and 1,5-linked arabinofuranose residues, but there was no alternation of these residues in the arabinan structure. Polysaccharide fractions scavenged the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical at 0.2–1.8 mg/mL. The correlation analysis showed that the DPPH scavenging activity of polysaccharide fractions was associated with the content of phenolic compounds (PCs).

Ultrasound-assisted enzyme extraction and properties of Shatian pomelo peel polysaccharide

“…The determination of DPPH radical scavenging capacity was referred to the method of Golovchenko et al [12] with modifications. 1 mL of StPP solution at different concentrations (0 mg/mL, 0.1 mg/mL, 0.2 mg/mL, 0.4 mg/mL, 0.8 mg/mL, 1.6 mg/mL, 3.2 mg/mL) was prepared and added to the same volume of 0.2 mmol/L DPPH-ethanol solution.…”

Optimization of Aqueous Two‐Phase Extraction of Polysaccharides from Hawthorn by Response Surface Methodology Coupled with Genetic Algorithm and Its Antioxidant Activity

“…As a nitrogen‐centered stable free radical, the maximum absorption of DPPH at 517 nm decreases with the addition of free radical scavenger, which is widely used for the evaluation of free radical elimination ability in vitro. [ 19 ] The scavenging ability of HPs and ascorbic acid on DPPH free radical is shown in Figure A. Figure 5A shows that the scavenging ability of HPs and ascorbic acid in the range of 2–64 mg mL −1 to DPPH free radical increased with the increase of their mass concentration, and showed a dose‐dependent manner.…”

“…According to the method described by Golovchenko et al. (2020), [ 19 ] the 1,1‐diphenyl‐2‐picryl‐hyd (DPPH) powder was dissolved in ethanol and prepared into 0.2 mmol L −1 DPPH solution, and then different concentrations (2, 4, 8, 16, 32, and 64 mg mL −1 ) of HPs were prepared. 100 µL different concentrations of HPs and ascorbic acid solution (control group) were placed into 96‐well plate, respectively, and then 100 µL DPPH solution (0.2 mmol L −1 ) was rapidly added to 96‐well plate, respectively.…”

“…(2020), [19] the 1,1-diphenyl-2-picryl-hyd (DPPH) powder was dissolved in ethanol and prepared into 0.…”

Structural Characterization, In Vitro Digestion Property, and Biological Activity of Sweet Corn Cob Polysaccharide Iron (III) Complexes

“…The concentration of free radicals at 50% scavenging (IC 50 ) was also predicted. Equal concentrations of SCCP were prepared and measured in the same manner; the same concentration of ascorbic acid served as a positive control [ 52 ]. where A 0 is the absorbance without the sample, A 1 is the absorbance of the polysaccharide-iron (III) complexes, and A 2 is the absorbance without the DPPH-ethanol solution.…”