Polysialic acid glycomimetics promote myelination and functional recovery after peripheral nerve injury in mice

Functional Role of the Interaction between Polysialic Acid and Myristoylated Alanine-rich C Kinase Substrate at the Plasma Membrane

“…2B). In addition, we could also show that a PSA-mimicking peptide (25) binds to MARCKS-ED peptide, whereas a scrambled version of the PSA-mimicking peptide did not bind the MARCKS-ED peptide (Fig. 2B).…”

“…Upon conformational change, PSA-NCAM may trigger signal transduction events and neurite outgrowth directly or indirectly through an interaction with an associated or nearby binding partner. Because only NCAM and/or the fibroblast growth factor receptor have been suggested to be involved in PSA signaling (25,42), we believe that the promotion of neurite outgrowth by MARCKS-ED peptide is mediated by NCAM and may involve the fibroblast growth factor receptor.…”

“…Colominic acid, chondroitin sulfate, and heparin were purchased from Sigma. PSAmimicking peptide with the sequence NTHTDPYIYPID and a scrambled version of this peptide with the sequence TNYDIT-PPHDIYC have been described (25). MARCKS-ED peptide (KKKKKRFSFKKSFKLSGFSFKKNKK) and the corresponding control peptide (KKKKKRASAKKSAKLSGASAKKNKK; sequence differences are underlined) as well as two peptides deriving from brain acid soluble protein 1 (BASP1) and comprising putative effector domains similar to that present in MARCKS (BASP1-ED peptide 1, EEKPKDAADGEAKAEEKE-ADKAAAAKEAPKA; BASP1-ED peptide 2, GGKLSKKKKGY-NVNDEKAKDKDKKA) were purchased from Schafer-N (Copenhagen, Denmark).…”

Histological analysis of low-intensity laser therapy effects in peripheral nerve regeneration in Wistar rats

“…Therefore, numerous studies have searched for the optimization of the regeneration of peripheral nerves utilizing different techniques, always aiming to abbreviate the period of recovery of the injured nerve 3,4 . Thus, the model of crush lesion has been adopted by several authors that emphasize the fact that the crush preserves, at least in part, the nerve structure, facilitating its regeneration, and for not demonstrating the necessity of realizing a suture, what implicates in previous training in microsurgical techniques and the availability of adequate instrumental and material, in general, with high cost 1 .…”

Embryonic Stem Cells Overexpressing the Recognition Molecules L1 and Tenascin-R Enhance Regeneration in Mouse Models of Acute and Chronic Neurological Disorders

“…Since these PSA-expressing ES cells were transplanted into the striatum of healthy unlesioned rats, it is not known how these cells influence function in animal models of neurological diseases. However, such transplantation studies could be very promising given that PSA glycomimetica promote functional recovery in mice after peripheral nerve injury (Mehanna et al, 2009) and spinal cord compression (Mehanna et al 2010). ECM molecules can act as attractant or repellent guidance molecules and both functions have been described for TNR in vitro and in vivo (Schachner et al, 1994;Jones and Jones, 2000).…”