Polysiloxane graft poly(2‐vinylpyridine) copolymer‐based capillary coating for separation of dsDNA

Abstract

Polyether-modified polysiloxane graft poly(2-vinylpyridine) (PMP-g-P2VP) copolymer, which was confirmed by NMR spectra and GPC, was synthesized for separation of dsDNA by capillary electrophoresis. EOF measurement results showed that the synthesized copolymer-coated capillaries in this report could suppress EOF effectively compared to the bare fused-silica capillaries. The effectiveness of PMP-g-P2VP polymer as a media for DNA analysis was discussed. Significant improvement in resolution of dsDNA analysis was demonstrated compared to the conventional polymer matrix. Results showed that 100-bp DNA ladder samples achieved separation in a 30 cm effective capillary length at 2.0% concentration by using this copolymer. Finally, the relationships between morphologies of copolymer in glass surface and the separation efficiencies were studied by scanning electron microscope (SEM).