Polysomes, Stress Granules, and Processing Bodies: A Dynamic Triumvirate Controlling Cytoplasmic mRNA Fate and Function

Chantarachot, Thanin; Bailey‐Serres, Julia

doi:10.1104/pp.17.01468

Cited by 179 publications

(207 citation statements)

References 144 publications

(160 reference statements)

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“…The qRT‐PCR results indicated that PEAMT1 expression was very low in dpr2 roots relative to WT (Fig. S1c), which was possibly controlled by non‐sense‐mediated mRNA decay or other mechanism (Chantarachot & Bailey‐Serres, ). This locus, previously designated XIPOTL1 (Cruz‐Ramírez et al ., ; Alatorre‐Cobos et al ., ) and PEAMT1 (Craddock et al ., ) elsewhere, encodes an Arabidopsis PEAMT (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The qRT-PCR results indicated that PEAMT1 expression was very low in dpr2 roots relative to WT (Fig. S1c), which was possibly controlled by non-sense-mediated mRNA decay or other mechanism (Chantarachot & Bailey-Serres, 2018 S1d). By introducing a translational fusion PEAMT1 pro : PEAMT1 cds -GFP, the primary root growth and RAM maintenance phenotypes of dpr2 were completely rescued ( Fig.…”

Section: Researchmentioning

confidence: 99%

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Phosphoethanolamine N‐methyltransferase 1 contributes to maintenance of root apical meristem by affecting ROS and auxin‐regulated cell differentiation in Arabidopsis

et al. 2019

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Summary The continuous growth of roots requires the balance between cell division and differentiation. Reactive oxygen species (ROS) and auxin are important regulators of root development by affecting cell division and differentiation. The mechanism controlling the coordination of cell division and differentiation is not well understood. Using a forward genetic screen, we isolated a mutant, defective primary root 2 (dpr2), defective in root apical meristem (RAM) maintenance. The DPR2 gene encodes phosphoethanolamine N‐methyltransferase 1 (PEAMT1) that catalyzes phosphocholine biosynthesis in Arabidopsis. We characterized the primary root phenotypes of dpr2 using various marker lines, using histochemical and pharmacological analysis to probe early root development. Loss‐of‐function of DPR2/PEAMT1 resulted in RAM consumption by affecting root stem cell niche, division zone, elongation and differentiation zone (EDZ). PIN‐FORMED (PIN) protein abundance, PIN2 polar distribution and general endocytosis were impaired in the root tip of dpr2. Excess hydrogen peroxide and auxin accumulate in the EDZ of dpr2, leading to RAM consumption by accelerating cell differentiation. Suppression of ROS over‐accumulation or inhibition of auxin signalling partially prevent RAM differentiation in dpr2 after choline starvation. Taken together, we conclude that the EDZ of the root tip is most sensitive to choline shortage, leading to RAM consumption through an ROS–auxin regulation module.

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Section: Resultsmentioning

confidence: 99%

Section: Researchmentioning

confidence: 99%

Phosphoethanolamine N‐methyltransferase 1 contributes to maintenance of root apical meristem by affecting ROS and auxin‐regulated cell differentiation in Arabidopsis

et al. 2019

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“…belongs to a large RNA-binding protein family characterized by the presence of well-conserved RRM2-RRM3 motives (Chantarachot & Bailey-Serres, 2018). Indeed, in our AP 343 rosette analysis, PAB2 and PAB8 were reproducibly found in the 2',3'-cAMP eluate, whereas…”

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confidence: 88%

Interaction of 2',3'-cAMP with Rbp47b plays a role in stress granule formation

et al. 2018

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“…Unlike the case for animal and yeast cells, no complete proteome composition has been reported for plant SGs. The current list of known Arabidopsis SG components is no longer than 25 proteins, and these were identified (mostly) based on their homology with animal and yeast SGs (Chantarachot & Bailey‐Serres, ). We have addressed this gap in our understanding of plant SGs by adapting a protocol used for SG isolation from yeast and HeLa cells (Jain et al ., ) that takes advantage of SG marker lines that overexpress the RNA‐binding protein Rbp47b (AT3G19130) fused to GFP (Gutiérrez‐Beltrán et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Protein and metabolite composition of Arabidopsis stress granules

et al. 2019

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Summary Stress granules (SGs) are evolutionary conserved aggregates of proteins and untranslated mRNAs formed in response to stress. Despite their importance for stress adaptation, no complete proteome composition has been reported for plant SGs. In this study, we addressed the existing gap. Importantly, we also provide evidence for metabolite sequestration within the SGs. To isolate SGs we used Arabidopsis seedlings expressing green fluorescent protein (GFP) fusion of the SGs marker protein, Rbp47b, and an experimental protocol combining differential centrifugation with affinity purification (AP). SGs isolates were analysed using mass spectrometry‐based proteomics and metabolomics. A quarter of the identified proteins constituted known or predicted SG components. Intriguingly, the remaining proteins were enriched in key enzymes and regulators, such as cyclin‐dependent kinase A (CDKA), that mediate plant responses to stress. In addition to proteins, nucleotides, amino acids and phospholipids also accumulated in SGs. Taken together, our results indicated the presence of a preexisting SG protein interaction network; an evolutionary conservation of the proteins involved in SG assembly and dynamics; an important role for SGs in moderation of stress responses by selective storage of proteins and metabolites.

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Polysomes, Stress Granules, and Processing Bodies: A Dynamic Triumvirate Controlling Cytoplasmic mRNA Fate and Function

Cited by 179 publications

References 144 publications

Phosphoethanolamine N‐methyltransferase 1 contributes to maintenance of root apical meristem by affecting ROS and auxin‐regulated cell differentiation in Arabidopsis

Phosphoethanolamine N‐methyltransferase 1 contributes to maintenance of root apical meristem by affecting ROS and auxin‐regulated cell differentiation in Arabidopsis

Interaction of 2',3'-cAMP with Rbp47b plays a role in stress granule formation

Protein and metabolite composition of Arabidopsis stress granules

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