Polystyrene replicas of neuronal basal lamina act as excellent guides for regenerating neurites

Karlsson, Martin; Johansson, Fredrik; Kanje, Martin

doi:10.1016/j.actbio.2011.03.029

Cited by 9 publications

(9 citation statements)

References 28 publications

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“…En un contexto biológico más amplio, se ha demostrado que la unión célula-laminina in vitro afecta a fenómenos tan diversos como la migración celular, la división de la difusión, y el mantenimiento del fenotipo diferenciado (Timpl & Brown, 1994;Karlsson et al, 2011).…”

Section: El Papel De La Lámina Basalunclassified

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Regeneración de las lesiones críticas del nervio periférico con factores de crecimiento. Estudio experimental

Medrano

Sanz

Pérez

et al. 2013

Revista Española de Cirugía Ortopédica y Traumatología

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Section: El Papel De La Lámina Basalunclassified

“…Presumiblemente la lámina basal pueda someterse a una reparación con el tiempo, debido a que sus bucles redundantes asociados con bandas de Büngner recién denervadas están ausentes en el endoneuro de nervios crónicamente denervados (Feneley et al, 1991;Karlsson et al, 2011).…”

Section: El Papel De La Lámina Basalunclassified

Regeneración de las lesiones críticas del nervio periférico con factores de crecimiento. Estudio experimental

Medrano

Sanz

Pérez

et al. 2013

Revista Española de Cirugía Ortopédica y Traumatología

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“…In the context of axon regeneration, certain molecules containing the basal lamina, particularly fibronectin, laminin and heparan sulfate promote axonal elongation in vitro and in vivo [1][2][3][4][5][6][7][8] In a broader biological context it has been shown that cell-laminin binding in vitro affects phenomena as diverse as cell migration, cell division, and maintenance of the differentiated phenotype [9,10].…”

Section: Introductionmentioning

confidence: 99%

The Role of the Basal Lamina in Nerve Regeneration

García-Medrano¹,

Cs²,

Pb³

et al. 2016

J Cytol Histol

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Introduction: In the context of axon regeneration, certain molecules containing the basal lamina, particularly fibronectin, laminin and heparin sulfate promote axonal elongation in vitro and in vivo. This structure could lead the axons to distal stump in nerve defect repair. Material and Methods: In twenty male Wistar rats, a critical defect of 15 mm was created in the sciatic nerve, which was bridged by an acellular muscle graft, which were obtained from the gluteus medius muscle of two donor rats, and chemically treated to cellular extraction by protocol consisted of 7 h in distilled water changed three times every 2-3 h, one night in Triton detergent, and 24 h in deoxycholate. After 90 days, the explanted pieces of sciatic nerve with grafts were carved and cut into seven pieces equally in all animals; pieces were processed in resin (toluidine blue staining) and also for paraffin embedding: hematoxylin-eosin and Masson trichrome. Results: Microscopically, proximal ends appeared as a normal nerve, with proper, regular and orderly fascicular distribution, perfectly defined by its three wraps, with little connective tissue. At the graft, regenerated axons used the muscle as a bridge, with many small nerve fascicles separated by host tissue and plenty of blood vessels. Axons followed their basal lamina, defining small fascicles with large vessels and abundant connective tissue. At the distal end, there was no a clear division in fascicles in some animals. There was little connective tissue between the fibers and the epineurium was quite developed. Conclusions: The selected biological method for chemical treatment of skeletal muscle achieves acellular grafts. We successfully could repair a critical lesion of the sciatic nerve in rats using this acellular muscle graft. Acellular muscle that we employed as a graft provides a bridge tool for the advancement of the regenerating axons, perhaps due to the structural proximity of laminin to nerve sheaths.

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“…It is possible that such a scaffold may provide a better support for regenerating axons if inserted into the gap between the nerve stumps using the tubulization technique. Thus, polystyrene scaffolds, produced so as to replicate the basal lamina of peripheral nerves, lead to a neurite alignment parallel to the structure of the basal-lamina like tubes obtained in the preparation (Karlsson et al 2011). …”

Section: Introductionmentioning

confidence: 99%

Enhanced peripheral nerve regeneration by the combination of a polycaprolactone tubular prosthesis and a scaffold of collagen with supramolecular organization

et al. 2013

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The purpose of this study was to investigate the influence of implanting collagen with a supramolecular organization on peripheral nerve regeneration, using the sciatic nerve tubulization technique. For this purpose, adult female Sprague Dawley rats were divided into five groups: (1) TP – sciatic nerve repaired with empty polyethylene tubular prothesis (n = 10), (2) TPCL – nerve repair with empty polycaprolactone (PCL) tubing (n = 8), (3) TPCLF – repair with PCL tubing filled with an implant of collagen with a supramolecular organization (n = 10), (4) AG – animals that received a peripheral nerve autograft (n = 8), and (5) Normal nerves (n = 8). The results were assessed by quantification of the regenerated fibers, nerve morphometry, and transmission electron microscopy, 60 days after surgery. Immunohistochemistry and polarization microscopy were also used to analyze the regenerated nerve structure and cellular elements. The results showed that the AG group presented a larger number of regenerated axons. However, the TPCL and TPCLF groups presented more compact regenerated fibers with a morphometric profile closer to normal, both at the tube midpoint and 2 mm distal to the prosthesis. These findings were reinforced by polarization microscopy, which indicated a better collagen/axons suprastructural organization in the TPCLF derived samples. In addition, the immunohistochemical results obtained using the antibody anti-p75NTR as a Schwann cell reactivity marker demonstrated that the Schwann cells were more reactive during the regenerative process in the TPCLF group as compared to the TPCL group and the normal sciatic nerve. Altogether, the results of this study indicated that the implant of collagen with a supramolecular organization positively influenced and stimulated the regeneration process through the nerve gap, resulting in the formation of a better morphologically arranged tissue.

show abstract

Polystyrene replicas of neuronal basal lamina act as excellent guides for regenerating neurites

Cited by 9 publications

References 28 publications

Regeneración de las lesiones críticas del nervio periférico con factores de crecimiento. Estudio experimental

Regeneración de las lesiones críticas del nervio periférico con factores de crecimiento. Estudio experimental

The Role of the Basal Lamina in Nerve Regeneration

Enhanced peripheral nerve regeneration by the combination of a polycaprolactone tubular prosthesis and a scaffold of collagen with supramolecular organization

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