Polyunsaturated fatty acids of germ cell membranes, glutathione and blutathione-dependent enzyme-PHGPx: from basic to clinic

Lenzi, Andrea; Gandini, L.; Lombardo, Francesco; Picardo, Mauro; Maresca, Vittoria; Panfili, Enrico; Tramer, Federica; Boitani, Carla; Dondero, F.

doi:10.1016/s0010-7824(02)00276-7

Cited by 91 publications

(61 citation statements)

References 10 publications

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“…Free radicals cause membrane and macromolecule damage by lipid peroxidation . The structure of the male germ cell membrane is rich in polyunsaturated fatty acids and thereby is mostly prone to lipid peroxidation . The observed elevation of oxidative and inflammatory biomarkers was reported in previous studies …”

Section: Discussionmentioning

confidence: 99%

Ellagic acid and rosmarinic acid attenuate doxorubicin-induced testicular injury in rats

Georgy

Maher²

2017

J Biochem Mol Toxicol

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The anticancer drug doxorubicin causes testicular toxicity as an undesirable effect. The present study was undertaken to investigate the possible protection of ellagic acid and rosmarinic acid during doxorubicin administration. For this purpose eight groups of male Sprague-Dawley rats were used (n = 10), one group received vehicle served as control, and other groups received 5 mg/kg doxorubicin twice a week for 2 weeks for a cumulative dose of 20 mg/kg, ellagic acid (10 mg/kg/day, 14 consecutive days p.o.), rosmarinic acid (75 mg/kg/day, 14 consecutive days p.o.), ellagic acid and rosmarinic acid. The latter three regimens were given to control and doxorubicin-received rats. Doxorubicin decreased testicular relative weight, sperm count, motility, serum testosterone, testicular glycogen, and sialic acid with increased incidence of histopathological changes, oxidative stress, tumor necrosis factor-alpha, as well as cholinesterase activity. Conversely, ellagic and rosmarinic acid treatment ameliorated such damage, thus showing the possibility to use as an adjuvant during doxorubicin treatment.

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Section: Discussionmentioning

confidence: 99%

Ellagic acid and rosmarinic acid attenuate doxorubicin-induced testicular injury in rats

Georgy

Maher²

2017

J Biochem Mol Toxicol

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“…The released ROS will accumulate and multiply in quantity due to the presence of large amount of unsaturated fatty acid (Lenzi et al, 1996;Lenzi et al, 2002). ROS from other sources including electron leakage from the spermatozoa mitochondria would also further worsen this problem.…”

Section: Discussionmentioning

confidence: 99%

Albumin-V: Role in Bovine Spermatozoa Viability, Motility and Membrane Elasticity

Ibrahim¹,

Johor²,

JOthman³

et al. 2013

JAS

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Semen preservation is an important process to ensure semen quality is sufficient for assisted reproductive technology. Studies have showed that suppression of free radicals, crystal formation and regulation of extracellular osmotic pressure is essential to ensure survivability. Past studies have indicated that utilizing protein already existent in the body could be used as a free radical scavenger and osmotic regulator -a potential preservation agent. Thus, the objective of this study was to determine the effect of different albumin-V concentrations on pooled bovine spermatozoa. Pooled cryopreserved semen straws were thawed and incubated in different concentrations of albumin-V (0, 0.2, 0.4, 0.6, 0.8, 1.0 mg albumin-V/ml Bio-excel ® extender) at 4°C. Sperm motility and viability were analyzed at 0, 2, 24 and 48 hours using Makler Chamber. Results indicated that motility and viability were noticeably reduced against time except for sperm incubated in 0.4 mg/ml. Membrane elasticity study using atomic force microscope (AFM) was then conducted on 0, 0.4, and 1.0 mg/ml 48 hours spermatozoa. We had observed that sperm membrane in the 0.4 mg/ml had a lower elasticity value (1.7 ± 0.6 kPA) compared to fresh semen (5.5 ± 1.0 kPA), 0 mg/ml (5.5 ± 0.1 kPA) and 1.0 mg/ml (5.3 ± 0.9 mg/ml). It is very likely that the motility and viability reduction was partly due to the changes in spermatozoa membrane elasticity. We conclude that albumin-V is a potential molecule for 4°C semen preservation; varying albumin concentration will have an influence on the spermatozoa membrane and affect the overall outcome of semen quality. Further study is required to elucidate the function and mechanism of albumin-V in preservation and its direct effect on ART outcome.

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“…Oxidative stress is a state related to increased cell damage triggered by oxygen and oxygen-derived free radicals known as reactive oxygen species (ROS). Due to the high levels of polyunsaturated fatty acids in plasma membranes, spermatozoa of both mammals (Lenzi et al 2002) and fish (Pustowka et al 2000) are particularly susceptible to oxidative damage. Imbalance between ROS and sperm antioxidant activity has been reported to be a primary cause of spermatozoon damage (Li et al 2010).…”

Section: Doi: 1017221/89/2017-cjasmentioning

confidence: 99%

Oxidative stress and antioxidant enzyme defence system in seminal plasma of common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) during spawning season

Shaliutina‐Kolešová¹,

Rui²,

Ashtiani³

et al. 2018

CZECH J ANIM SCI

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Shaliutina-Kolešová A., Rui N., Ashtiani S., Rodina M., Cosson J., Linhart O. (2018): Oxidative stress and antioxidant enzyme defence system in seminal plasma of common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) during spawning season. Czech J. Anim. Sci., 63, 78-84.Assessment of seminal oxidative stress indicators is emerging as a valuable prognostic tool in assisted reproductive technology. We investigated levels of oxidative stress caused by reactive oxygen species (ROS) and the antioxidant enzyme defence system comprising superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxidase (GPx) in seminal plasma (SP) of common carp Cyprinus carpio and rainbow trout Oncorhynchus mykiss over the course of the spawning season. Oxidation was determined in lipids and proteins by assessing thiobarbituric acid reactive species (TBARS) and the 2,4-dinitrophenylhydrazine carbonyl groups, respectively. Levels of SOD were assessed by the autoxidation of pyrogallol. Determination of GR and GPx was based on the rate of NADPH oxidation. We observed clear alterations in lipids and proteins over the course of the spawning season in both species. The highest levels of TBARS and CP were recorded late in the season. SOD was not significantly altered in either species. The activity of GR was higher in carp SP late in spawning compared to other times (P < 0.05), while in rainbow trout, GR significantly increased (24.13 ± 2.8 mU/mg protein) in mid-season. A significantly lower GPx activity (9.18 ± 1.32 mU/mg protein) was found in rainbow trout SP early in the spawning season, but no significant differences in GPx were observed over the course of the season in carp. These results provide further understanding of the role of fish SP antioxidants and present new data on the oxidant and antioxidant balance in SP during the spawning season that may be of value in the development of methods for artificial reproduction of teleost species.

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Polyunsaturated fatty acids of germ cell membranes, glutathione and blutathione-dependent enzyme-PHGPx: from basic to clinic

Cited by 91 publications

References 10 publications

Ellagic acid and rosmarinic acid attenuate doxorubicin-induced testicular injury in rats

Ellagic acid and rosmarinic acid attenuate doxorubicin-induced testicular injury in rats

Albumin-V: Role in Bovine Spermatozoa Viability, Motility and Membrane Elasticity

Oxidative stress and antioxidant enzyme defence system in seminal plasma of common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) during spawning season

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