Polyurethane/poly(lactic-co-glycolic) acid composite scaffolds fabricated by thermally induced phase separation

Rowlands, Andrew; Lim, Sooyoung; Martin, Darren J.; Cooper-White, Justin J.

doi:10.1016/j.biomaterials.2006.12.032

Cited by 126 publications

(72 citation statements)

References 40 publications

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“…The solid formed in the polymer-rich phase is imbued with crystals formed in the polymer-poor phase. These crystals are removed, leaving a highly porous structure (more than 90 %) [149][150][151]. A composite scaffold is fabricated using the TIPS process from chemically very different poly(lactic-co-glycolic) (PLGA) and BioPU copolymers.…”

Section: Fabrication Of Porous Biopusmentioning

confidence: 99%

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Synthesis and structure-property relationships of biodegradable polyurethanes

Pergal¹,

Blaban²

2017

Biodegradable Polymers: Recent Developments and New Perspectives

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Section: Fabrication Of Porous Biopusmentioning

confidence: 99%

“…Pure PU scaffolds have an isotropic emulsion-like structure, a porous Young's modulus of 15.7 kPa and are much more elastic than PLGA scaffolds. On the other hand, the composite PLGA / PU scaffold exhibits advantageous morphological, mechanical, cell adhesion and growthsupporting properties [149].…”

Section: Fabrication Of Porous Biopusmentioning

confidence: 99%

Synthesis and structure-property relationships of biodegradable polyurethanes

Pergal¹,

Blaban²

2017

Biodegradable Polymers: Recent Developments and New Perspectives

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“…The polymer-rich phase solidifies, whilst the polymer-poor phase crystallizes. The formed crystals are removed, leaving a highly porous structure (more than 90 %) [13,14].The structure of the scaffold obtained in this way depends on the polymer solution concentration, the quenching temperature and the quenching rate [15]. Solvents application was recognized as the main disadvantage of this technique.…”

Section: Introductionmentioning

confidence: 99%

Ascorbic Acid in Polyurethane Systems for Tissue Engineering

Kucińska-Lipka¹,

St.²,

Janik³

et al. 2016

ChChT

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Abstract. The introduction of the paper was devoted to the main items of Tissue Engineering (TE) and the way of porous structure obtaining as scaffolds. Furthermore, the significant role of the scaffold design in TE was described. It was shown, that properly designed polyurethanes (PURs) find application in TE due to the proper physicochemical, mechanical and biological properties. Then the use of L-ascorbic acid (L-AA) in PUR systems for TE was described. L-AA has been applied in this area due to its suitable biological characteristics and antioxidative properties. Moreover, L-AA influences tissue regeneration due to improving collagen synthesis, which is a primary component of the extracellular matrix (ECM). Modification of PUR with L-AA leads to the materials with higher biocompatibility and such system is promising for TE applications.

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“…Blending PU with other biocompatible scaffolding materials such as PLLA has yielded 3-D matrices having improved mechanical properties and cell binding. [20][21][22] However, their closeness to in vivo nanofibrous nature or its molecular mechanism, leading to HSC survival, and CAMDR mechanisms similar to those seen in vivo is not shown. 22 Unquestionably, the basic nature of ECM is nanofibrous and that creation of a nanofibrous matrix has potential significance in artificially recreating any aspect of the ECM.…”

Section: Introductionmentioning

confidence: 99%

“…[20][21][22] However, their closeness to in vivo nanofibrous nature or its molecular mechanism, leading to HSC survival, and CAMDR mechanisms similar to those seen in vivo is not shown. 22 Unquestionably, the basic nature of ECM is nanofibrous and that creation of a nanofibrous matrix has potential significance in artificially recreating any aspect of the ECM. 23 Thus, we revisited PLLA's ability to yield nanofibrous structures and PU's ability to generate micro structures using thermally induced phase separation (TIPS) technology.…”

Section: Introductionmentioning

confidence: 99%

Development and molecular characterization of polymeric micro-nanofibrous scaffold of a defined 3-D niche for in vitro chemosensitivity analysis against acute myeloid leukemia cells

Nair¹,

Mony²,

Menon³

et al. 2015

IJN

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Standard in vitro drug testing employs 2-D tissue culture plate systems to test anti-leukemic drugs against cell adhesion-mediated drug-resistant leukemic cells that harbor in 3-D bone marrow microenvironments. This drawback necessitates the fabrication of 3-D scaffolds that have cell adhesion-mediated drug-resistant properties similar to in vivo niches. We therefore aimed at exploiting the known property of polyurethane (PU)/poly- l -lactic acid (PLLA) in forming a micro-nanofibrous structure to fabricate unique, not presented before, as far as we are aware, 3-D micro-nanofibrous scaffold composites using a thermally induced phase separation technique. Among the different combinations of PU/PLLA composites generated, the unique PU/PLLA 60:40 composite displayed micro-nanofibrous morphology similar to decellularized bone marrow with increased protein and fibronectin adsorption. Culturing of acute myeloid leukemia (AML) KG1a cells in FN-coated PU/PLLA 60:40 shows increased cell adhesion and cell adhesion-mediated drug resistance to the drugs cytarabine and daunorubicin without changing the original CD34 + /CD38 − /CD33 − phenotype for 168 hours compared to fibronectin tissue culture plate systems. Molecularly, as seen in vivo, increased chemoresistance is associated with the upregulation of anti-apoptotic Bcl2 and the cell cycle regulatory protein p27 Kip1 leading to cell growth arrest. Abrogation of Bcl2 activity by the Bcl2-specific inhibitor ABT 737 led to cell death in the presence of both cytarabine and daunorubicin, demonstrating that the cell adhesion-mediated drug resistance induced by Bcl2 and p27 Kip1 in the scaffold was similar to that seen in vivo. These results thus show the utility of a platform technology, wherein drug testing can be performed before administering to patients without the necessity for stromal cells.

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Polyurethane/poly(lactic-co-glycolic) acid composite scaffolds fabricated by thermally induced phase separation

Cited by 126 publications

References 40 publications

Synthesis and structure-property relationships of biodegradable polyurethanes

Synthesis and structure-property relationships of biodegradable polyurethanes

Ascorbic Acid in Polyurethane Systems for Tissue Engineering

Development and molecular characterization of polymeric micro-nanofibrous scaffold of a defined 3-D niche for in vitro chemosensitivity analysis against acute myeloid leukemia cells

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Polyurethane/poly(lactic-co-glycolic) acid composite scaffolds fabricated by thermally induced phase separation

Cited by 126 publications

References 40 publications

Synthesis and structure-property relationships of biodegradable polyurethanes

Synthesis and structure-property relationships of biodegradable polyurethanes

Ascorbic Acid in Polyurethane Systems for Tissue Engineering

Development and molecular characterization of&nbsp;polymeric micro-nanofibrous scaffold of a defined 3-D niche for in vitro chemosensitivity analysis against acute myeloid leukemia cells

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Product

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About

Development and molecular characterization of polymeric micro-nanofibrous scaffold of a defined 3-D niche for in vitro chemosensitivity analysis against acute myeloid leukemia cells