Polyvalent spherical aptamer engineered macrophages: X-ray-actuated phenotypic transformation for tumor immunotherapy

Chen, Yuanyuan; Gao, Peng; Pan, Wei; Shi, Mingwan; Liu, Shujie; Li, Na; Tang, Bo

doi:10.1039/d1sc03997k

Cited by 22 publications

(16 citation statements)

References 45 publications

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“…Alternatively, Chen et al proposed polyvalent spherical aptamers (PSAs) as a macrophage engineering strategy [ 94 ]. PSAs were generated through the functionalization of gold nanoparticles with both the thiol-modified AS1411 aptamer and a DNA linker that carries, at the free extremity, a functional group for reacting with azide tags created on M0 macrophages through the abovementioned metabolic labeling and biorthogonal click reactions ( Figure 6 ).…”

Section: Aptamer-based Immune Strategies For Tnbc Treatmentmentioning

confidence: 99%

Aptamer-Based Strategies to Boost Immunotherapy in TNBC

Agnello

d’Argenio

Nilo

et al. 2023

Cancers

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The immune system (IS) may play a crucial role in preventing tumor development and progression, leading, over the last years, to the development of effective cancer immunotherapies. Nevertheless, immune evasion, the capability of tumors to circumvent destructive host immunity, remains one of the main obstacles to overcome for maximizing treatment success. In this context, promising strategies aimed at reshaping the tumor immune microenvironment and promoting antitumor immunity are rapidly emerging. Triple-negative breast cancer (TNBC), an aggressive breast cancer subtype with poor outcomes, is highly immunogenic, suggesting immunotherapy is a viable strategy. As evidence of this, already, two immunotherapies have recently become the standard of care for patients with PD-L1 expressing tumors, which, however, represent a low percentage of patients, making more active immunotherapeutic approaches necessary. Aptamers are short, highly structured, single-stranded oligonucleotides that bind to their protein targets at high affinity and specificity. They are used for therapeutic purposes in the same way as monoclonal antibodies; thus, various aptamer-based strategies are being actively explored to stimulate the IS’s response against cancer cells. The aim of this review is to discuss the potential of the recently reported aptamer-based approaches to boost the IS to fight TNBC.

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Section: Aptamer-based Immune Strategies For Tnbc Treatmentmentioning

confidence: 99%

Aptamer-Based Strategies to Boost Immunotherapy in TNBC

Agnello

d’Argenio

Nilo

et al. 2023

Cancers

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“…For example, the sgc8c aptamer adopts a hairpin structure to bind with the PTK-7 protein, and the AS1411 aptamer uses a Gquadruplex structure to bind with nucleolin (Figure 1D). 26,27 Specially, stimuli-responsive moieties were further incorporated into these aptamers to modulate their binding to the targets. For example, an i-motif modification endowed a sgc8c aptamer with pH responsiveness 28 and photocaged thymidine endowed a thrombin aptamer with light responsiveness.…”

Section: Dynamic Dna Nanostructuresmentioning

confidence: 99%

“…In addition, the mismatch between T-T and C-C base pairs can be induced by silver cations (Ag + ) and mercury cations (Hg 2+ ), generating ion-bridging structures in dsDNA (Figure C). , Aptamers can also recognize targets (e.g., ATP, proteins, exosomes, and cells) through conformational changes. For example, the sgc8c aptamer adopts a hairpin structure to bind with the PTK-7 protein, and the AS1411 aptamer uses a G-quadruplex structure to bind with nucleolin (Figure D). , Specially, stimuli-responsive moieties were further incorporated into these aptamers to modulate their binding to the targets. For example, an i-motif modification endowed a sgc8c aptamer with pH responsiveness and photocaged thymidine endowed a thrombin aptamer with light responsiveness .…”

Section: Dynamic Dna Nanostructuresmentioning

confidence: 99%

Dynamic DNA Nanostructures for Cell Manipulation

2023

ACS Biomater. Sci. Eng.

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Dynamic DNA nanostructures are DNA nanostructures with reconfigurable elements that can undergo structural transformations in response to specific stimuli. Thus, anchoring dynamic DNA nanostructures on cell membranes is an attractive and promising strategy for well-controlled cell manipulation. Here, we review the latest progress in dynamic DNA nanostructures for cell manipulation. Commonly used mechanisms for dynamic DNA nanostructures are first introduced. Subsequently, we summarize the anchoring strategies for dynamic DNA nanostructures on cell membranes and list possible applications (including programming cell membrane receptors, controlling ligand activity and drug delivery, capturing and releasing cells, and assembling cells into clusters). Finally, insights into the remaining challenges are presented.

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“…However, native EVs have difficulty meeting the functional requirements of the complex physiological environment; therefore, necessary engineering design and modification can significantly improve the performance of EVs as a therapeutic system. As biological macromolecules, nucleic acid has unique biological functions and has been widely used in the field of nanomedicine [10][11][12][13] . RNA interference, antisense oligonucleotides, and cluster regularly spaced short palindromic repeats-associated protein 9 (CRISPR/Cas9) system can downregulate, enhance, or correct gene expression and have wide application potential in gene therapy research [14][15][16][17][18][19] .…”

Section: Introductionmentioning

confidence: 99%

Nucleic acid functionalized extracellular vesicles as promising therapeutic systems for nanomedicine

Liu¹,

He²,

Cen³

et al. 2022

EVCNA

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Extracellular vesicles (EVs), as natural carriers, are regarded as a new star in nanomedicine due to their excellent biocompatibility, fascinating physicochemical properties, and unique biological regulatory functions. However, there are still some challenges to using natural EVs, including poor targeting ability and the clearance from circulation, which may limit their further development and clinical use. Nucleic acid has the functions of programmability, targeting, gene therapy, and immune regulation. Owing to the engineering design and modification by integrating functional nucleic acid, EVs offer excellent performances as a therapeutic system in vivo. This review briefly introduces the function and mechanism of nucleic acid in the diagnosis and treatment of diseases. Then, the strategies of nucleic acid-functionalized EVs are summarized and the latest progress of nucleic acid-functionalized EVs in nanomedicine is highlighted. Finally, the challenges and prospects of nucleic acid-functionalized EVs as a promising diagnostic system are proposed.

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Polyvalent spherical aptamer engineered macrophages: X-ray-actuated phenotypic transformation for tumor immunotherapy

Abstract: Spatiotemporally activatable immune cells are promising for tumor immunotherapy owing to their potential high specificity and low side effects. Herein, we developed an X-ray-induced phenotypic transformation (X-PT) strategy through macrophage...

Cited by 22 publications

References 45 publications

Aptamer-Based Strategies to Boost Immunotherapy in TNBC

Aptamer-Based Strategies to Boost Immunotherapy in TNBC

Dynamic DNA Nanostructures for Cell Manipulation

Nucleic acid functionalized extracellular vesicles as promising therapeutic systems for nanomedicine

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