Polyvinyl alcohol scaffolds and supplementation support 3D and sphere culturing of human cancer cell lines by reducing apoptosis and promoting cellular proliferation

Okita, Yukari; Zheng, Ling; Kawanishi, Kunio; Miyoshi, Hirotoshi; Yanagihara, Kazuyoshi; Kato, Mitsuyasu

doi:10.1111/gtc.12843

Cited by 8 publications

(5 citation statements)

References 28 publications

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“…A similar result was also found in the 3D culture of human breast cancer Hs578T cells and human pancreatic cancer cell lines (Sui67 and Sui72) with PVA hydrogels. This result further indicates that PVA hydrogels can reduce cancer cell apoptosis and promote cancer cellular proliferation [169].…”

Section: D Cell Culturesmentioning

confidence: 85%

“…Have the good mechanical strength to provide structural support for various cell types in 3D cell culture PVA mHSCs [166], mSCCs [167], human glioma cell lines LN299, U87MG and Gli36 [168], human breast cancer Hs578T cells, and human pancreatic cancer cell lines Sui67 and Sui72 [169] Enhance the expansion of murine hematopoietic stem cells (mHSCs) [166]; promote the meiotic and post-meiotic differentiation rate of mSCCs [167]; form tumor spheroids [168,169].…”

Section: Syntheticmentioning

confidence: 99%

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Hydrogels: Properties and Applications in Biomedicine

et al. 2022

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Hydrogels are crosslinked polymer chains with three-dimensional (3D) network structures, which can absorb relatively large amounts of fluid. Because of the high water content, soft structure, and porosity of hydrogels, they closely resemble living tissues. Research in recent years shows that hydrogels have been applied in various fields, such as agriculture, biomaterials, the food industry, drug delivery, tissue engineering, and regenerative medicine. Along with the underlying technology improvements of hydrogel development, hydrogels can be expected to be applied in more fields. Although not all hydrogels have good biodegradability and biocompatibility, such as synthetic hydrogels (polyvinyl alcohol, polyacrylamide, polyethylene glycol hydrogels, etc.), their biodegradability and biocompatibility can be adjusted by modification of their functional group or incorporation of natural polymers. Hence, scientists are still interested in the biomedical applications of hydrogels due to their creative adjustability for different uses. In this review, we first introduce the basic information of hydrogels, such as structure, classification, and synthesis. Then, we further describe the recent applications of hydrogels in 3D cell cultures, drug delivery, wound dressing, and tissue engineering.

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Section: D Cell Culturesmentioning

confidence: 85%

Section: Syntheticmentioning

confidence: 99%

Hydrogels: Properties and Applications in Biomedicine

et al. 2022

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“…Mechanically confined fibroblast has shown the ability to reprogram in stem-cell-like cells and rejuvenate [ 71 , 72 ]. In the literature, PVA and bacterial cellulose also demonstrated spheroid formation and long-term stem-cell maintenance properties [ 29 , 73 , 74 ]. The potential of these spheroid-like structures at the surface of our DAC/PVA material is very high, providing an environment recapitulating the in-vivo complexity better than the traditional 2D or some 3D cultures.…”

Section: Discussionmentioning

confidence: 99%

Cellulosic/Polyvinyl Alcohol Composite Hydrogel: Synthesis, Characterization and Applications in Tissue Engineering

et al. 2021

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The biomedical field still requires composite materials for medical devices and tissue engineering model design. As part of the pursuit of non-animal and non-proteic scaffolds, we propose here a cellulose-based material. In this study, 9%, 18% and 36% dialdehyde-functionalized microcrystalline celluloses (DAC) were synthesized by sodium periodate oxidation. The latter was subsequently coupled to PVA at ratios 1:2, 1:1 and 2:1 by dissolving in N-methyl pyrrolidone and lithium chloride. Moulding and successive rehydration in ethanol and water baths formed soft hydrogels. While oxidation effectiveness was confirmed by dialdehyde content determination for all DAC, we observed increasing hydrolysis associated with particle fragmentation. Imaging, FTIR and XDR analysis highlighted an intertwined DAC/PVA network mainly supported by electrostatic interactions, hemiacetal and acetal linkage. To meet tissue engineering requirements, an interconnected porosity was optimized using 0–50 µm salts. While the role of DAC in strengthening the hydrogel was identified, the oxidation ratio of DAC showed no distinct trend. DAC 9% material exhibited the highest indirect and direct cytocompatibility creating spheroid-like structures. DAC/PVA hydrogels showed physical stability and acceptability in vivo that led us to propose our DAC 9%/PVA based material for soft tissue graft application.

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“…The 3D cultivation of human pancreatic cancer cell lines (Sui67 and Sui72) and human breast cancer Hs578T cells with PVA hydrogels produced results that were comparable. This finding demonstrates that PVA hydrogels can inhibit cancer cell apoptosis and stimulate cancer cell growth [ 76 ].…”

Section: Classification Of Hydrogelsmentioning

confidence: 94%

Hydrogels—A Promising Materials for 3D Printing Technology

Kaliaraj

Shanmugam

Dasan

et al. 2023

Gels

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Hydrogels are a promising material for a variety of applications after appropriate functional and structural design, which alters the physicochemical properties and cell signaling pathways of the hydrogels. Over the past few decades, considerable scientific research has made breakthroughs in a variety of applications such as pharmaceuticals, biotechnology, agriculture, biosensors, bioseparation, defense, and cosmetics. In the present review, different classifications of hydrogels and their limitations have been discussed. In addition, techniques involved in improving the physical, mechanical, and biological properties of hydrogels by admixing various organic and inorganic materials are explored. Future 3D printing technology will substantially advance the ability to pattern molecules, cells, and organs. With significant potential for producing living tissue structures or organs, hydrogels can successfully print mammalian cells and retain their functionalities. Furthermore, recent advances in functional hydrogels such as photo- and pH-responsive hydrogels and drug-delivery hydrogels are discussed in detail for biomedical applications.

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Polyvinyl alcohol scaffolds and supplementation support 3D and sphere culturing of human cancer cell lines by reducing apoptosis and promoting cellular proliferation

Cited by 8 publications

References 28 publications

Hydrogels: Properties and Applications in Biomedicine

Hydrogels: Properties and Applications in Biomedicine

Cellulosic/Polyvinyl Alcohol Composite Hydrogel: Synthesis, Characterization and Applications in Tissue Engineering

Hydrogels—A Promising Materials for 3D Printing Technology

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