Polyvinylchloride (PVC) particles implantation in mouse liver. A technique for experimental study of schistosome eggs-induced liver pathology

Joky, Alexandre; Cornu, Marjorie; Louis, Dominique; Ja, Grimaud

doi:10.1007/bf01935985

Cited by 7 publications

(2 citation statements)

References 6 publications

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“…Unloaded beads served as negative controls. Beads were injected into the caecal vein of each mouse according to the technique described by Joky et al (1978) and Van Marck et al (1980). The animals were killed at days 3, 8, 16, and 32 after injection.…”

Section: Animalsmentioning

confidence: 99%

Expression of intercellular adhesion molecule-1 and lymphocytefunction-associated antigen-1 in experimental Schistosoma mansoni infection and in synchronous periparticular hepatic granulomas in mice: immunohistochemistry, confocal laser scanning microscopy, and immunoelectron microscopy

Jacobs¹,

Bogers²,

Deelder

et al. 1997

Parasitology Research

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Adhesion molecules play an important role in inflammatory and immunological responses. We assessed the expression pattern of intercellular adhesion molecule-1 (ICAM-1) and lymphocytefunction-associated antigen-1 (LFA-1) in the livers of mice experimentally infected with Schistosoma mansoni and in synchronous hepatic granulomas induced by injection of soluble egg antigen (SEA)-coupled Sepharose beads in a mesenteric vein of mice. By immunohistochemistry, confocal laser scanning microscopy, and immunoelectron microscopy, ICAM-1 was localized on endothelial cells, sinusoidal-lining cells (Kupffer cells and sinusoidal endothelium), the hepatocyte cell membrane facing Disse's space, and inflammatory cells in the granuloma. LFA-1 was visualized on the inflammatory cells of the granuloma and on phagocytic sinusoidal-lining cells, most likely Kupffer cells. ICAM-1- and LFA-1-immunoreactive cells were present in the granuloma as early as at 3 days after injection of SEA-coupled beads and persisted with time. As granulomas became older, nonimmunoreactive granuloma cells appeared. We conclude that adhesion molecules play an important role in the genesis of the schistosomal granuloma.

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Section: Animalsmentioning

confidence: 99%

Expression of intercellular adhesion molecule-1 and lymphocytefunction-associated antigen-1 in experimental Schistosoma mansoni infection and in synchronous periparticular hepatic granulomas in mice: immunohistochemistry, confocal laser scanning microscopy, and immunoelectron microscopy

Jacobs¹,

Bogers²,

Deelder

et al. 1997

Parasitology Research

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“…Significant shunting of 15 /tm microspheres (about the diameter of lung schistosomula) was observed only in rats exposed to F. hepatica. Although the mean percentage shunting observed was only 15 %, compared with the mean percentage resistance of 67 %, this may reflect the fact that schistosomula are not inert, but are capable of migrating along the lumina of blood vessels, Since even slight changes in the dimensions of conformation of hepatic blood vessels may facilitate the escape of schistosomula, alterations in the intrahepatic microvasculature may also contribute to this shunting effect by producing what has been termed a 'leaky liver' (Joky, Cornu, Louis & Grimaud, 1978;Wilson ct al. 1983).…”

Section: Discussionmentioning

confidence: 92%

Studies on heterologous resistance betweenSchistosoma mansoniandFasciola hepaticain inbred rats

et al. 1987

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Fischer rats infected with Fasciola hepatica showed significant resistance to Schistosoma mansoni challenge, and vice versa, whereas immunization with 20 Krad-irradiated S. mansoni cercariae failed to protect against F. hepatica challenge, but did protect against homologous challenge. When groups of rats received intraperitoneal implants of newly excysted juvenile flukes, 20- to 22-day-old juveniles, or 8- to 10-week-old flukes, none was significantly protected against S. mansoni challenge, whereas juvenile implants did protect against homologous F. hepatica challenge. In passive transfer experiments in rats, serum from F. hepatica-infected rats or rabbits protected recipients against homologous, but not heterologous challenge, and serum from rats vaccinated with 20 Krad-irradiated S. mansoni cercariae protected recipients against homologous, but not heterologous challenge. These experiments provide evidence that the mechanisms involved in homologous and heterologous resistance are different, the latter lacking immunological specificity. Microsphere injections in F. hepatica-infected rats demonstrated 'shunting' from the portal system to the systemic circulation. If migrating schistosomula are also 'shunted' in Fasciola-infected rats, this, rather than immunologically specific effector mechanisms, might explain their failure to establish in the portal system.

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Induction of experimental murine granuloma formation againstSchistosoma japonicum eggs produced by in vitro ova deposition, in vitro tissue extraction, or lyophilization

et al. 1991

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Fresh eggs, laid by Schistosoma japonicum adult worms in vitro or recovered from tissue after trypsin digestion, as well as non-viable lyophilized eggs were injected into mice via the cecal vein. The freshly laid eggs induced a marked, maximal reaction at 2 weeks after implantation. This reaction was indistinguishable from that seen early in naturally infected mice. Lyophilized eggs induced granulomas only one-tenth the size of those formed around freshly laid eggs. Maximal granulomatous reactivity was not seen until the 4th week after implantation and the intensity of cellular reactivity and associated histopathological change was much lower than that observed with freshly laid eggs. Reactions against live, tissue-extracted eggs were quantitatively and temporally intermediate between those observed against laid and those surrounding lyophilized eggs. The results suggests that these differences in granuloma formation are due to variable quantities of locally produced cytotoxic materials and/or antigen that stimulate immune reactions of different intensities.

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Polyvinylchloride (PVC) particles implantation in mouse liver. A technique for experimental study of schistosome eggs-induced liver pathology

Cited by 7 publications

References 6 publications

Expression of intercellular adhesion molecule-1 and lymphocytefunction-associated antigen-1 in experimental Schistosoma mansoni infection and in synchronous periparticular hepatic granulomas in mice: immunohistochemistry, confocal laser scanning microscopy, and immunoelectron microscopy

Expression of intercellular adhesion molecule-1 and lymphocytefunction-associated antigen-1 in experimental Schistosoma mansoni infection and in synchronous periparticular hepatic granulomas in mice: immunohistochemistry, confocal laser scanning microscopy, and immunoelectron microscopy

Studies on heterologous resistance betweenSchistosoma mansoniandFasciola hepaticain inbred rats

Induction of experimental murine granuloma formation againstSchistosoma japonicum eggs produced by in vitro ova deposition, in vitro tissue extraction, or lyophilization

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