Polyyne-Enriched Extract from Oplopanax elatus Significantly Ameliorates the Progression of Colon Carcinogenesis in ApcMin/+ Mice

Abstract

Colorectal cancer (CRC) is the third most common cancer in the world. Oplopanax elatus is widely used in traditional medicine. However, little is known about its pharmacological effects and bioactive compounds. We evaluated the effects of the polyyne-enriched extract from O. elatus (PEO) on the progression of colon carcinogenesis in ApcMin/+ mice. In addition, these effects were also investigated in HCT116 and SW480 cells. After PEO oral administration (0.2% diet) for 12 weeks, PEO significantly improved body weight changes and reduced the tumor burden and tumor multiplicity compared with the untreated mice. Meanwhile, western blot and immunohistochemistry results showed PEO significantly reduced the expression of β-catenin and cyclinD1 in both small intestine and the colon tissues compared with the untreated mice. In addition, PEO treatment significant decreased the cell viability in both HCT116 and SW480 cell lines. It also decreased the levels of β-catenin, cyclinD1, c-myc and p-GSK-3β in HCT116 and SW480 cells at 25 μM. These results indicate that PEO may have potential value in prevention of colon cancer by down-regulating Wnt-related protein.

The active fraction of Garcinia yunnanensis suppresses the progression of colorectal carcinoma by interfering with tumorassociated macrophage‐associated M2 macrophage polarization in vivo and in vitro

“…The mice were sacrificed by cervical dislocation. The whole intestine was removed immediately after sacrifice and opened longitudinally after washing with ice‐cold phosphate‐buffered saline (PBS) as previously described 26 . The number, location, and size of the visible tumors throughout the intestine were measured to calculate the incidence of adenoma.…”

“…The whole intestine was removed immediately after sacrifice and opened longitudinally after washing with ice-cold phosphate-buffered saline (PBS) as previously described. 26 The number, location, and size of the visible tumors throughout the intestine were measured to calculate the incidence of adenoma. The tumor numbers were counted and grouped based on sizes as follows: <2 mm, 2-4 mm and >4 mm.…”

YYFZBJS ameliorates colorectal cancer progression in ApcMin/+ mice by remodeling gut microbiota and inhibiting regulatory T-cell generation

“…Mice were sacrificed by cervical dislocation. The whole intestine was removed immediately after sacrifice and opened longitudinally after washed with ice-cold PBS as previously described [37]. The number, location, and size of visible tumors throughout the intestine were measured to calculate the incidence of adenoma.…”

Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota

“…To date, polyynes have been chiefly reported with high contents in the root of O. elatus ( Huang et al, 2014a ). Increasing attention has been paid to two main polyynes facarindiol (FAD) and oplopandiol (OPD), because of their significant anti-tumor activities ( Purup, Larsen & Christensen, 2009 ; Qiao et al, 2017 ; Sun et al, 2016 ). However, most studies remain focused on the pharmacological and chemical constituents of O. elatus , while its metabolic profiles are rather obscured.…”