Pome fruit viruses at the Canadian Clonal Genebank and molecular characterization of Apple chlorotic leaf spot virus isolates

Wang, L.P.; Hóng, Ní; Matić, S.; Myrta, A.; Song, Yali; Michelutti, R.; Wang, G.P.

doi:10.1016/j.scienta.2011.08.001

Cited by 12 publications

(8 citation statements)

References 36 publications

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“…Plant Pathology (2014) 63, 63-71 evaluate ACLSV prevalence in the Mediterranean region including Greece (Varveri & Bem, 1995) were mainly based on serological methods which have been shown here and elsewhere (Myrta et al, 2003;Spiegel et al, 2006;Wang et al, 2011) to have limited reliability. In Greece, the prevalence of the virus has been recently studied by using molecular methods (RT-PCR) in pome fruits and Prunus cerasifera and almonds (Charou et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…ACLSV occurrence in pome (72·7% in apple) and stone (33·3% in peach) fruit species during the survey highlights the necessity for the production of certified propagated plant material (Rowhani et al ., ). Studies to evaluate ACLSV prevalence in the Mediterranean region including Greece (Varveri & Bem, ) were mainly based on serological methods which have been shown here and elsewhere (Myrta et al ., ; Spiegel et al ., ; Wang et al ., ) to have limited reliability. In Greece, the prevalence of the virus has been recently studied by using molecular methods (RT‐PCR) in pome fruits and Prunus cerasifera (Mathioudakis et al ., ) and almonds (Charou et al ., ).…”

Section: Discussionmentioning

confidence: 99%

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Host‐range studies, genetic diversity and evolutionary relationships of ACLSV isolates from ornamental, wild and cultivated Rosaceous species

et al. 2013

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A large-scale survey was carried out to study the host range and genetic diversity of Apple chlorotic leaf spot virus (ACLSV) in various Rosaceae species, with a special emphasis on ornamentals and wild shrubs. Samples were tested by DAS-ELISA using two different antisera, and RT-PCR amplification of part of the CP gene. There was generally a poor correlation between the results obtained with the two sets of serological reagents and between serological and molecular detection assays. Using a nested RT-PCR assay developed here, ACLSV was found to be widespread among cultivated, ornamental and wild species of the Rosaceae. The virus was detected for the first time in plum, wild cherry, Crataegus monogyna, Prunus spinosa and Prunus cerasifera in Greece. Sequences of a part of the CP encoding gene and the 3′ untranslated region from ACLSV isolates originating from various wild species and ornamentals were compared to those of isolates from cultivated hosts, showing similar divergence levels. Further phylogenetic analysis using the sequenced region indicated that the isolates from wild or ornamental hosts were not more closely related to each other than to isolates from cultivated hosts. The possible role of different factors in the spread of ACLSV on cultivated, ornamental and wild species is discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Host‐range studies, genetic diversity and evolutionary relationships of ACLSV isolates from ornamental, wild and cultivated Rosaceous species

et al. 2013

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“…More recently, a large number of studies were performed for biological characteristics and strain characteristics of ACLSV in different fruit trees. [ 5 , 14 , 15 ] However, little information on the molecular characterization was obtained for apple trees from the Jiaodong area of Shandong Province. Our research provided the ACLSV sequences by means of RT-PCR.…”

Section: Resultsmentioning

confidence: 99%

“…ACLSV consists of numerous strains that produce different symptoms in sensitive fruit tree cultivars, whereas other strains are latent in many apple and pear cultivars. [ 5 ] Therefore, the fast and effective measures of detection are particularly important.…”

Section: Introductionmentioning

confidence: 99%

“…[ 8–10 ] In recent years, RT-PCR techniques have been used to detect the economically important and widespread viruses such as ACLSV, apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), etc. [ 5 , 11–13 ] The present work utilized RT-PCR to assess seven apple accessions at the Jiaodong Peninsula orchard. The results would contribute to the molecular biology information of ACLSV and provide theoretical foundation for virus-free seedling cultivation in the Jiaodong region, China.…”

Section: Introductionmentioning

confidence: 99%

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The RT-PCR identification and sequence analysis of Apple chlorotic leaf spot virus from apple cultivars in Jiaodong Peninsula, China

Hu¹,

Wang²,

Jiang³

et al. 2014

Biotechnology & Biotechnological Equipment

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A set of specific primer pairs was utilized to detect Apple chlorotic leaf spot virus (ACLSV) from seven different apple cultivars in Jiaodong Peninsula via reverse transcription polymerase chain reaction (RT-PCR), and the sequence of ACLSV genome was analysed. The results indicate that: (1) High-purity total RNA could be successfully isolated using plant RNA rapid extraction kit. The ratios of A260/A280 varied between 1.8 and 2.1. The fragmentation in agarose gel was good and the 28S and 16S bands were clear, which suggested that the extracted RNA had better quality and could be used for RT-PCR. (2) The amplified products by RT-PCR were approximately 220 bp, which showed the tested samples were infected by ACLSV in this study. (3) Sequencing analysis showed that the lengths of the target fragments were 217 bp, and the sequence identity rate ranged from 85.7% to 99.1% at the nucleotide level aligned with the corresponding sequences of other ACLSV strains in National Center for Biotechnology Information.

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Tube-Capture (TC) RT-PCR and Multiplex RT-PCR for Diagnosis and Characterization of Viruses in Fruit Trees

Wang

2021

Methods in Molecular Biology

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Pome fruit viruses at the Canadian Clonal Genebank and molecular characterization of Apple chlorotic leaf spot virus isolates

Cited by 12 publications

References 36 publications

Host‐range studies, genetic diversity and evolutionary relationships of ACLSV isolates from ornamental, wild and cultivated Rosaceous species

Host‐range studies, genetic diversity and evolutionary relationships of ACLSV isolates from ornamental, wild and cultivated Rosaceous species

The RT-PCR identification and sequence analysis of Apple chlorotic leaf spot virus from apple cultivars in Jiaodong Peninsula, China

Tube-Capture (TC) RT-PCR and Multiplex RT-PCR for Diagnosis and Characterization of Viruses in Fruit Trees

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