Pomegranate dieback caused by Lasiodiplodia gilanensis in California

Úrbez-Torres, J. R.; Hand, Francesca Peduto; Trouillas, Florent P.; Gubler, W. D.

doi:10.1007/s10658-016-1071-y

Cited by 9 publications

(8 citation statements)

References 20 publications

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“…Grapevine samples were frrst surfacesterilized by submerging them in 4.5% sodium hypochlorite for 5 min and washed twice with sterile distilled water After air-drying, sampled vines were inspected for presence of necrotic roots, and if detected, fungi from roots were isolated as described by Petit and Gubler (32). Additionally, samples were sectioned to reveal the vascular symptoms in the trunk, and fungi associated with symptomatic tissue were isolated as described by Úrbez-Torres et al (45). Small pieces of symptomatic tissues from trunk or roots were incubated on potato dextrose agar (PDA) (Difco, Detroit, MI) plates amended with tetracycline hydrochloride (0.01%) (SigmaAldrich, St Louis, MO) (PDA-tet) at 20°C in the dark until fungal colonies were observed.…”

Section: Iviaterials and Iviethodsmentioning

confidence: 99%

“…In the first experiment, dormant canes of Chardonnay were collected frotii an experimental vineyard located at the Pacific Agri-Food Research Centre in Sumtnerland in mid-March of 2012. Six canes per fungal isolate were inoculated using 10-day-old myceliurn plugs as described by LJrbez-Torres et al (45). Twelve additional canes were inoculated with noncolonized PDA plugs for negative controls.…”

Section: Pathogenicity Studiesmentioning

confidence: 99%

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Grapevine Trunk Diseases in British Columbia: Incidence and Characterization of the Fungal Pathogens Associated with Black Foot Disease of Grapevine

et al. 2014

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Urbez-Torres, J. R., Haag, P., Bowen, P., and O'Gorman, D. T. 2014. Grapevine trunk diseases in Britisb Columbia: Incidence and cbaracterization of tbe fungal pathogens associated witb black foot disease of grapevine. Plant Dis. 98:456-468.Black foot disease of grapevines, caused by several fungal species in the genera Campyloearpon, Cylindrocarpon, CylindrocladieUa, and Ilyonectria, causes significant economic losses to the grapevine industry worldwide. This study represents the first attempt to identify and characterize the fungal pathogens associated with black foot disease of grapevines in British Columbia (BC). Field surveys conducted tbroughout all grape-growing regions in BC tbat included assessment of foliar symptomatology and isolations from symptomatic vines sbowed CyUndrocarponlllyonectria spp. occurred in 32 of 90 (35.5%) young vineyai-ds surveyed (<8 year old) and in 41 of 215 (19%) samples collected. In 20 of the 41 (48.8%) samples, Cylindrocarpon/ Ilyonectria spp. were the sole fungi isolated from symptomatic tissue.In the rest of the samples, black foot fungi were found to primarily coexist with fungal taxa associated with Petri disease of grapevines. Colony and conidia phenotypical characterization, along with DNA analyses of the internal transcribed spacer region (ITS1-5.8S-ITS2) of the rDNA, and part of the ß-tubulin and translation elongation factor 1-a genes, revealed five different black foot fungi occurring in declining young vines in BC, namely Cylindrocarpon pauciseptatum, Ilyonectria Uriodendri, Ilyonectria macrodidyma, Ilyonectria robusta, and Ilyonectria torresensis. Pathogenicity studies showed all five species to be highly virulent in the grapevine rootstock cultivar 3309C. Overall, /. uriodendri and /. macrodidyma were the most virulent species when inoculated in Vitis vinifera 'Chardonnay' and rootstock 3309C.

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Section: Iviaterials and Iviethodsmentioning

confidence: 99%

Section: Pathogenicity Studiesmentioning

confidence: 99%

Grapevine Trunk Diseases in British Columbia: Incidence and Characterization of the Fungal Pathogens Associated with Black Foot Disease of Grapevine

et al. 2014

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“…The main Tunisian producing regions are Gabes and Gafsa oases, Cap Bon, Bizerte and Sousse where pomegranate plays an important ecological and socio-economic role [4,5]. In Tunisia, as well as in many other countries [6], pomegranate has traditionally been considered a hardy tree, suffering from few diseases as compared to most fruit trees. Nevertheless, in the last decade, many fungal diseases were reported in many regions of the globe and were currently classified as one of the most limiting factors for pomegranate cultivation.…”

Section: Introductionmentioning

confidence: 99%

“…For instance, wilt due to Ceratocystis fimbriata [7], wood canker and branch dieback caused by Cytospora punicae [8], and root and crown rot incited by Phytophthora palmivora [9] were detected in many countries. Also, Lasiodiplodia gilanensis, associated to severe dieback of one to multiple branches in California [6], and Neofusicoccum parvum, involved in shoot blight and canker symptoms, are reported to occur on pomegranate trees in Greece [10]. Several fungi are also common pomegranate fruit rot pathogens such as Alternaria spp.…”

Section: Introductionmentioning

confidence: 99%

Coniella granati (Saccardo) a New Potential Threat to Pomegranate (Punica granatum L.) in Tunisia Causing Twig Dieback and Fruit Rot

Khiareddine¹,

Ibrahim²,

Abdallah³

et al. 2018

J Plant Pathol Microbiol

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The disease caused by Coniella granati (Saccardo) (syn. Pilidiella granati, Saccardo) is an expanding threat to pomegranate (Punica granatum L.) cultivation and industry and is rapidly emerging in almost all pomegranateproducing regions of the world. The current study presents the first attempt to characterize C. granati associated with pomegranate dieback, shoot blight and fruit rot in Tunisia. Pathogenic isolates were identified based on their cultural and morphological characteristics and molecular data. Collected C. granati isolates were shown able to grow between 10°C and 30°C with an optimal mycelial growth at 20-25°C but they did not grow at 35°C. The fungus was able to grow 4-10 pH with an optimum growth at pH 4-5 for Cg1 and Cg2 isolates. Potato Dextrose Agar (PDA), Carrot Agar (CA) and Oatmeal Agar (OA) followed by pomegranate juice agar (PJA) and Malt Extract Agar (MEA) favored its mycelial growth. Pathogen growth was reduced under continuous light in comparison with the 12 h light/12 h dark regime and was significantly slowest under 24 h dark regime. Inoculated to pomegranate cv. Gabsi fruits, C. granati isolates induced soft rot within 9 days following incubation at 25°C and a complete fruit rot after 15 days. Leaves were highly susceptible to C. granati infection and completely degenerated 5 days post-inoculation. C. granati isolates were found to be pathogenic on pomegranate cv. Gabsi attached shoots and detached branches, giving rise to brown necrotic lesions. Keeping in view the importance of the pomegranate crop and the destructive nature of Coniella induced disease, further studies are needed to verify the pathogen host range, its aggressiveness towards the mostly grown Tunisian pomegranate varieties and to search for suitable control methods.

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“…In particular, L. crassispora T. I. Burgess & Barber , L. exigua and L. mediterranea Linaldeddu , Deidda & Berraf ‐ Tebbal , L. missouriana Ú rbez ‐ Torres , Peduto & Gubler , L. parva A. J. L. Phillips , A. Alves & Crous , L. pseudotheobromae A. J. L. Phillips , A. Alves & Crous , L. theobromae and L. viticola Ú rbez ‐ Torres , Peduto & Gubler have been associated with declining grapevines.…”

Section: Introductionmentioning

confidence: 99%

Lasiolactols A and B Produced by the Grapevine Fungal Pathogen Lasiodiplodia mediterranea

Andolfi

Basso

Giambra

et al. 2016

Chemistry & Biodiversity

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A strain of Lasiodiplodia mediterranea, a fungus associated with grapevine decline in Sicily, produced several metabolites in liquid medium. Two new dimeric γ‐lactols, lasiolactols A and B (1 and 2), were characterized as (2S*,3S*,4R*,5R*,2′S*,3′S*,4′R*,5′R*)‐ and (2R*,3S*,4R*,5R*,2′R*,3′S*,4′R*,5′R*)‐(5‐(4‐hydroxymethyl‐3,5‐dimethyl‐tetrahydro‐furan‐2‐yloxy)‐2,4‐dimethyl‐tetrahydro‐furan‐3‐yl]‐methanols by IR, 1D‐ and 2D‐NMR, and HR‐ESI‐MS. Other four metabolites were identified as botryosphaeriodiplodin, (5R)‐5‐hydroxylasiodiplodin, (–)‐(1R,2R)‐jasmonic acid, and (–)‐(3S,4R,5R)‐4‐hydroxymethyl‐3,5‐dimethyldihydro‐2‐furanone (3 – 6, resp.). The absolute configuration (R) at hydroxylated secondary C‐atom C(7) was also established for compound 3. The compounds 1 – 3, 5, and 6, tested for their phytotoxic activities to grapevine cv. Inzolia leaves at different concentrations (0.125, 0.25, 0.5, and 1 mg/ml) were phytotoxic and compound 5 showed the highest toxicity. All metabolites did not show in vitro antifungal activity against four plant pathogens.

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Pomegranate dieback caused by Lasiodiplodia gilanensis in California

Cited by 9 publications

References 20 publications

Grapevine Trunk Diseases in British Columbia: Incidence and Characterization of the Fungal Pathogens Associated with Black Foot Disease of Grapevine

Grapevine Trunk Diseases in British Columbia: Incidence and Characterization of the Fungal Pathogens Associated with Black Foot Disease of Grapevine

Coniella granati (Saccardo) a New Potential Threat to Pomegranate (Punica granatum L.) in Tunisia Causing Twig Dieback and Fruit Rot

Lasiolactols A and B Produced by the Grapevine Fungal Pathogen Lasiodiplodia mediterranea

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