Pomegranate inhibits neuroinflammation and amyloidogenesis in IL-1β-stimulated SK-N-SH cells

Abstract

Conclusions:Taken together, our study demonstrates that pomegranate inhibits inflammation, as well as amyloidogenesis in IL-1β-stimulated SK-N-SH cells. We propose that pomegranate is a potential nutritional strategy in slowing the progression of neurodegenerative disorders like Alzheimer's disease.

Antiinflammatory and anti‐cancer activities of pomegranate and its constituent, ellagic acid: Evidence from cellular, animal, and clinical studies

“…One hour after oral consumption of 180 mg of pomegranate juice, containing 25 mg EA and 318 mg Ellagitannin, the blood level of EA is achieved 31.9 mg/L in human (Velagapudi, Baco, Khela, Okorji, & Olajide, ). Shirode et al evaluated the effects of nano‐encapsulation of pomegranate active ingredients.…”

“…Velagapudi et al evaluated the effects of freeze‐dried pomegranate juice (PWE, 50–200 μg/mL) on IL‐1β‐stimulated human neuroblastoma SK‐N‐SH cells. They demonstrated that PWE markedly alleviates the PGE2 production, IκB and IKK phosphorylation, Beta‐secretase 1 (BACE‐1), COX‐2 and Aβ expression following IL‐1β‐induced inflammation in SK‐N‐SH cells (Velagapudi et al, ).…”

“…The amount of polyphenols in the peel (1.89 ± 0.1070 mg/g) is much higher than each of juice (0.063 ± 0.0003 mg/g) and seeds (0.051 ± 0.0075 mg/g) (Dikmen, Ozturk, & Ozturk, 2011). One hour after oral consumption of 180 mg of pomegranate juice, containing 25 mg EA and 318 mg Ellagitannin, the blood level of EA is achieved 31.9 mg/L in human (Velagapudi, Baco, Khela, Okorji, & Olajide, 2016). Shirode et al evaluated the effects of nanoencapsulation of pomegranate active ingredients.…”

“…Velagapudi et al evaluated the effects of freeze-dried pomegranate juice (PWE, 50-200 μg/mL) on IL-1β-stimulated human neuroblastoma SK-N-SH cells. They demonstrated that PWE markedlyalleviates the PGE2 production, IκB and IKK phosphorylation, Betasecretase 1 (BACE-1), COX-2 and Aβ expression following IL-1βinduced inflammation in SK-N-SH cells(Velagapudi et al, 2016).Rheumatoid fibroblast-like synoviocyte line. Seong and coworkers evaluated the antiinflammatory effects of delphinidin (DP) isolated from pomegranate, a novel histone acetyl-transferase inhibitor (HATi), on TNF-α-induced inflammation in human rheumatoid arthritis synovial MH7A cell line.…”

Clinical Applications of Pomegranate

“…In a dose of 1.5 mg/kg punicalagin attenuated LPS (250 μg/kg daily 7 times) induced memory impairment and blocked the LPS-induced expression of inflammatory proteins via suppression of NF-κB activation [80]. In addition, freeze-dried pomegranate (25-200 μg/ml) in a dose-dependent manner reduced COX-2-dependent prostaglandin E2 (PGE2) production in SK-N-SH cells stimulated with IL-1β [81]. The neuroprotective action of pomegranate was obscured in an animal study in which dietary supplementation of 4% pomegranate extract to APPsw/Tg2576 mice for 15 months ameliorated the loss of synaptic structure proteins, inhibited neuroinflammatory activity, and enhanced autophagy (degradation and recycling of cellular components).…”