Pomegranate Juice and Peel Extracts are Able to Inhibit Proliferation, Migration and Colony Formation of Prostate Cancer Cell Lines and Modulate the Akt/mTOR/S6K Signaling Pathway

Chaves, Fernanda Machado; Pavan, Isadora Carolina Betim; Silva, Luiz Guilherme Salvino da; Freitas, Lidia; Rostagno, Maurı́cio A.; Antunes, Adriane Elisabete Costa; Bezerra, Rosângela Maria Neves; Simabuco, Fernando Moreira

doi:10.1007/s11130-019-00776-0

Cited by 47 publications

(26 citation statements)

References 33 publications

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“…Interestingly, the wound healing and colony formation data demonstrated that that EEFS not only inhibited lung cancer cell metastasis, but also reduced tumorigenesis. These findings are in agreement with a previous report that pomegranate juice and peel extracts inhibited migration and colony formation in prostate cancer cells [36].…”

Section: Discussionsupporting

confidence: 94%

Bcl-2 is essential for Foeniculum vulgare seed extract-induced apoptosis in lung cancer

Zhao

2020

Preprint

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Background The factors behind the pathogenesis of lung cancer are not clear, and treatment failure is generally caused by drug resistance, recurrence, and metastasis. Development of new therapeutic agents to overcome drug-resistance remains a challenge clinically. Various extracts of Foeniculum vulgare have shown promising anticancer activity; however, effects on lung cancer and the underlying molecular mechanisms of action are not clear. Methods The cytotoxicity effects of EEFS were assessed by morphological changes or MTT assay. The BALB/c nude mice xenograft model was used for the in vivo study. Apoptotic ratio assay based on Annexin V-PI staining were measured by flow cytometry. Effect of EEFS on expression of apoptotic proteins was measured by Western blot. Mitochondria toxicity was evaluated by fluorescence to show membrane potential under a fluorescent microscope and Cytochrome C release. Results We found that the ethanol extract of Foeniculum vulgare seeds (EEFS) significantly reduced lung cancer cell growth in vitro and in vivo. EEFS decreased the viability of and triggered apoptosis in the lung cancer cell lines NCI-H446 and NCI-H661. EEFS induced apoptosis mainly through inhibition of Bcl-2 protein expression, reduction of mitochondrial membrane potential, and release of Cytochrome C. Moreover, EEFS significantly inhibited colony formation and cell migration in lung cancer cells. EEFS also effectively inhibited the growth of xenograft tumors derived from NCI-446 cells by reducing Bcl-2 protein expression and inducing apoptosis. Conclusions Taken together, these findings suggest that EEFS exerts anti-lung cancer activity by targeting the Bcl-2 protein and may have potential as a therapeutic drug for lung cancer.

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Section: Discussionsupporting

confidence: 94%

Bcl-2 is essential for Foeniculum vulgare seed extract-induced apoptosis in lung cancer

Zhao

2020

Preprint

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“…Differences in the molecular responses of PC‐3 and DU‐145 to the same treatment have already been pointed out in other studies. Chaves et al (2020) treated DU‐145 and PC‐3 cells with pomegranate juice and peel extract and observed a reduced modulation of proteins in the Akt/mTOR/S6K pathway in PC‐3 cells compared to DU‐145 cells, which may be explained by the fact that PC‐3 cells are knockout for PTEN and, therefore, present a more robust activation of the PI3K/Akt pathway (Chaves et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Beetroot and leaf extracts present protective effects against prostate cancer cells, inhibiting cell proliferation, migration, and growth signaling pathways

Silva

Fagundes

et al. 2021

Phytotherapy Research

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Beet (Beta vulgaris L.) has high nutritional value, containing bioactive compounds such as betalains and flavonoids. Scientific evidence points to the use of these natural compounds in the treatment of several types of cancer, such as prostate cancer, one of the main causes of morbidity and mortality in men. Here, we compared beet roots and leaves extracts, and their main compounds, apigenin, and betanin, respectively, in DU‐145 and PC‐3 prostate cancer cell lines. Both cells presented the proliferation decreased for beetroot and beet leaves extracts. The apigenin treatment also reduced the proliferation of both cell lines. Regarding cell migration, beet leaves extract was able to decrease the scratch area in both cell lines, whereas apigenin affected only PC‐3 cells' migration. In colony formation assay, both extracts were effective in reducing the number of colonies formed. Besides, the beet leaves extracts and apigenin presented strong inhibition of growth‐related signaling pathways in both cell lines, and the beetroot extract and betanin presented effects only in DU‐145 cells. Furthermore, the extracts and isolated compounds were able to reduce the levels of apoptotic and cell cycle proteins. This study reveals that beet extracts have important anti‐cancer effects against prostate cancer cells.

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“…Pomegranate peels represent 50% of total fruit weight and are a potential source of bioactive compounds, mainly phenolic compounds with a very broad spectrum of activity [ 13 ]. Indeed, several studies have reported the effects (antioxidant, anti-cancer, anti-inflammatory, lipid-lowering, anti-hypertensive) of phenolic extracts from the peel of the pomegranate, which makes them suitable as natural ingredients [ 13 , 14 , 15 , 16 , 17 ]. In addition, the natural product supplementation industry is increasingly interested in peel waste, manufacturing capsules containing concentrated phenolic compounds based on peels or whole fruit.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Effect of Moroccan Pomegranate (Punica granatum L. Sefri Variety) Extracts Rich in Punicalagin against the Oxidative Stress Process

Benchagra

Berrougui

Islam

et al. 2021

Foods

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Natural antioxidants products are widely distributed in food and medicinal plants. These natural antioxidants, especially polyphenols, exhibit a wide range of biological activities including anti-cancer, anti-inflammatory, and anti-atherosclerosis activities. Pomegranate (Punica granatum L.) is a rich source of polyphenolic components. The purpose of this study was to characterize the phenolic composition and flavonoids and anthocyanin content of different parts (peel and aril) of the Sefri variety of pomegranate. Our results showed that Peel extract was richer in these compounds than that of the Arils, especially in Punicalagin (A and B). DPPH free radical scavenging, reducing power (FRAP), β-carotene bleaching, and hydrogen peroxide scavenging assays revealed a greater dose-dependent activity of pomegranate peel phenolic extract (PPPE) compared to pomegranate aril phenolic extract (PAPE). PPPE was also more potent than PAPE concerning its ability to inhibit conjugated diene formation and to reduce α-tocopherol disappearance induced by CuSO4-mediated LDL peroxidation. Interestingly, both extracts (PPPE and PAPE) significantly inhibited lipid peroxidation and the formation of reactive oxygen species (ROS) in stressed J82 human bladder cancer cells. These results reflect the protective effects that this Moroccan variety of pomegranate can provide against the development of metabolic disorder, cancer, atherosclerosis, and cardiovascular disease. Given these properties, further studies should be undertaken to investigate possible applications of Sefri pomegranate extracts in the fields of food preservation and health supplements.

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Pomegranate Juice and Peel Extracts are Able to Inhibit Proliferation, Migration and Colony Formation of Prostate Cancer Cell Lines and Modulate the Akt/mTOR/S6K Signaling Pathway

Cited by 47 publications

References 33 publications

Bcl-2 is essential for Foeniculum vulgare seed extract-induced apoptosis in lung cancer

Bcl-2 is essential for Foeniculum vulgare seed extract-induced apoptosis in lung cancer

Beetroot and leaf extracts present protective effects against prostate cancer cells, inhibiting cell proliferation, migration, and growth signaling pathways

Antioxidant Effect of Moroccan Pomegranate (Punica granatum L. Sefri Variety) Extracts Rich in Punicalagin against the Oxidative Stress Process

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