Pomelo peels as alternative substrate for extracellular pectinase production by Aspergillus niger HFM-8

Abstract

The aim of this work was to develop an effective bioprocess to enhance the pectinase production by solid-state cultures of Aspergillus niger HFM-8. Methodology and results: The pectinase production produced by A. niger HFM-8 was studied under solid state fermentation using Malaysian pomelo (Citrus grandis) peel as the substrate. This local agricultural waste is rich with lignocellulolytic material including pectin acts as the inducer of pectinase production. Under optimized conditions, 5 g of 0.75 mm pomelo peel size, moisture content of 60% (v/w) sterile distilled water pH 5.0, inoculums size of 1x10 4 spores/mL, cultivation temperature of room temperature (30 ± 2 °C), no mixing incurred and with the addition of 1% (w/w) citrus pectin and 0.1% (w/w) urea has produced pectinase production of 306.89 U/g substrate and 0.78 mg glucosamine/g substrate of fungal growth on the 8 th day of cultivation. Conclusion, significance and impact of study: There was 48.82% increment in enzyme production after the improvement of parameters. It was found that pomelo peel is a suitable feedstock for pectinase production.

Storage of Beauveria bassiana Conidia Suspension: A Study Exploring the Potential Effects on Conidial iability and Virulence against Dermanyssus gallinae De Geer, 1778 Acari: Dermanyssidae

“…Additionally, although the agitation speed might enhance enzyme production by filamentous fungi, it might also damage the cells, interfering with fungal virulence, thus confirming our results [28].…”

Bio-catalysis of mango industrial waste by newly isolated Fusarium sp. (PSTF1) for pectinase production

“…(PSTF1) yielded the pectinase activity better than the reported strain. Ibrahim et al (2013) studied the effect of different inoculum sizes on pectinase production by Aspergillus niger HFD5A-1. Their results showed that the maximal production 1.64 U/ml at the inoculums size of 2 % (2/100 ml).…”

“…But when compared with other nitrogen source in presence of urea highest pectinase activity was observed. Similarly, Aspergillus niger HFM-8 has given highest pectinase production in presence of external nitrogen source urea with citrus pectin (Ibrahim et al 2013). Additional nutrients (other than substrate) in the growing media enhance the growth of fungi and growth related enzymes secretion.…”

Production of sorbitol by repeated batch fermentation using immobilized of Lactobacillus plantarum Strain (BAA-793) via Solid State Fermentation

“…Published by Rynnye Lyan Resources loss of desired biological activity is called immobilization of whole cells (Pilkingtonn et al, 1998;Ibrahim et al, 2013;Amenaghawon and Oiwoh, 2015). The immobilization of whole cells plays an important role in microbial ecology and is widespread in nature.…”

“…The immobilization of whole cells plays an important role in microbial ecology and is widespread in nature. Whole cell immobilization occurs to some extent in all microbe-based industrial processes as well, including water and wastewater treatment (Pilkingtonn et al, 1998;Ibrahim et al, 2013;Amenaghawon and Oiwoh, 2015). Before this, the immobilization of whole cells process and its application in the bioprocessing has been interesting for 30 years and it also has introduced in industrials (Behera et al, 2011).…”