Pontibacter beigongshangensis sp. nov., Isolated from the Mash of Wine

Xu, Jialiang; Sun, Leping; Sun, Zhan‐Bin; Xing, Xuan; Li, Jia; Lü, Xin; Huang, Ying; Ren, Qian

doi:10.1007/s00284-019-01782-w

“…Universal primers 27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-TACGGCTACCTTGTTACGACTT-3′) were used to amplify the bacterial 16S rRNA gene sequence. The 25 μl PCR amplification reaction system contained 12.5 μl of 2 × Pfu PCR Master Mix (TIANGEN Biotech Beijing Co., Ltd., Beijing, China), 7.5 μl of ddH 2 O, 3 μl of DNA template, 1 μl of primer 27F, and 1 μl of primer 1492R as described in a previous study ( Xu J. et al, 2019 ). The bacterial 16S rRNA gene was amplified according to the following procedure: 95°C for 5 min and 35 cycles at 95°C for 20 s, 55°C for 20 s, 72°C for 60 s, and 72°C for 5 min).…”

Section: Methodsmentioning

confidence: 99%

Culturing Bacteria From Fermentation Pit Muds of Baijiu With Culturomics and Amplicon-Based Metagenomic Approaches

Xu

¹

,

Sun

²

,

Xing

³

et al. 2020

Self Cite

View full text Add to dashboard Cite

The Baijiu-making microbiota has an important role in the alcohol production, flavor, and character of Baijiu. 16S rRNA gene sequencing revolutionized the understanding of Baijiu-making microbiota. In this study, nine phyla, 23 classes, 49 orders, 99 families, and 201 genera were detected in pit muds (PMs) by 16S rRNA gene sequencing. Firmicutes and Bacteroidetes predominated (>99%). At the order level, Clostridiales, Bacteroidales, and Bacillales predominated (>92%). At the genus level, Hydrogenispora , Petrimonas , Proteiniphilum , and Sedimentibacter predominated. The pure culture of Baijiu-making prokaryotes was essential to elucidating the role of these microbes in the fermentation of Baijiu. According to the theory of microbial culturomics, a culturing approach with multiple culture conditions was adopted, combining 16S rRNA gene sequencing. We identified 215 prokaryotic strains, which were assigned to 66 species, 41 genera, four phyla, and 19 potential new species. Gas conditions were key factors in culturomics. In addition, culturomics significantly increased the number of species isolated from the fermentation PM compared with previous reports. With culturomics, the diversity spectrum of culturable bacteria in the PM was increased 273.33% at the genus level. This study confirms the complementary role of culturomics in the exploration of complex microbiota.

show abstract

“…Sensitivity to antibiotics was detected by the disc-diffusion method after spreading cell suspensions on R2A agar plates and considered as susceptible when the diameter of the inhibition zone was over 1.2 cm. The tested antibiotics were (μg per disc, unless indicated): amikacin (30), amoxicillin (20), ampicillin (10), bacitracin (0.04 IU), carbenicillin (100), cefamezin (30), cefoxitin (30), cefradine (30), cephalexin (30), chloramphenicol (30), ciprofloxacin (5), clindamycin (2), doxycycline (30), erythromycin (15), gentamicin (10), kanamycin (30), lincomycin (2), minocycline (30), nalidixic acid (30), neomycin (30), norfloxacin (10), novobiocin (30), nystatin (100), ofloxacin (5), oxacillin (1), penicillin G (10 IU), polymyxin B (300 IU), rifampicin (5), streptomycin (10), tetracycline (30) and vancomycin [30,43].…”

Section: Physiological and Biochemical Characteristics Analysismentioning

confidence: 99%

“…As of May 2023, the genus Pontibacter comprised 42 validly published species (www.bacterio.net/genus/Pontibacter) [2]. Members of the genus Pontibacter are characterized as Gram-negative, rod-shaped, catalase-positive (except for Pontibacter qinzhouensis [3]), MK-7 as the predominant menaquinone, with very high amounts of iso-C 15 : 0 (except for Pontibacter aydingkolensis , Pontibacter beigongshangensis , Pontibacter deserti , Pontibacter niistensis and Pontibacter ummariensis [4–8]) and summed feature 4 (iso-C 17 : 1 I and/or anteiso-C 17 : 1 B), and a DNA G+C content range of 43.9–58.6 mol% [1, 3–39]. During investigation of the microbial diversity of the pit mud of the Chinese liquor, two novel strains (NBU2971 T and NBU2972 T ) were isolated.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pontibacter liquoris sp. nov. and Pontibacter vulgaris sp. nov., two novel bacteria isolated from the pit mud of Chinese liquor

Guo

¹

,

Wang

²

,

Liu

³

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

0

View full text Add to dashboard Cite

Two pink-pigmented bacterial strains, designated NBU2971T and NBU2972T, were isolated from the pit mud of a Chinese liquor. Phylogenetic analyses based on 16S rRNA gene sequences suggested that strains NBU2971T and NBU2972T formed a distinct lineage within the family Hymenobacteraceae and were closely related to members of the genus Pontibacter . 16S rRNA gene sequences revealed that strain NBU2971T showed highest similarity of 97.9 % to Pontibacter arcticus 2b14T, and strain NBU2972T showed the highest similarity of 96.9 % to Pontibacter deserti JC215T. The 16S rRNA gene sequence similarity, average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between the two novel strains were 95.2, 73.8 and 19.6 %, respectively, suggesting that they represent different species. The ANI and dDDH values between two novel strains and related species of genus Pontibacter were well below the threshold limit for prokaryotic species delineation. The genomic DNA G+C contents of strains NBU2971T and NBU2972T were 51.3 and 44.5 mol%, respectively. The major cellular fatty acids of the two novel strains were iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B). The major polar lipid of both novel strains was phosphatidylethanolamine. The only respiratory quinone was MK-7. Combining results of phenotypic, chemotaxonomic and genotypic data, strains NBU2971T and NBU2972T are considered to be two representatives in the genus Pontibacter , which the name Pontibacter liquoris sp. nov. and Pontibacter vulgaris sp. nov. are proposed. The type strains of the new species are NBU2971T (=KCTC 82916T=MCCC 1K06395T) and NBU2972T (=KCTC 82917T=MCCC 1K06396T), respectively.

show abstract

“…The DNA G+C content of members of the genus is in the range of 34.0-65.0 mol% [5]. Pontibacter species have been isolated from a variety of environments, such as soil [6,7], deserts [8], seawater [9,10], blood [11], wine mash [12] and rice paddy fields [13].…”

Section: Introductionmentioning

confidence: 99%

Pontibacter kalidii sp. nov., isolated from rhizosphere soil of Kalidium foliatum

Wang,

Jiang,

Osman

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

1

0

View full text Add to dashboard Cite

A Gram-negative, aerobic, gliding motile, rod-shaped bacterium, designated XAAS-72T, was isolated from the rhizosphere soil of Kalidium foliatum sampled in the Xinjiang Uyghur Autonomous Region, PR China. Cells grew at 4–45 °C, pH 5.0–8.0 and 0–8% NaCl, with optimal growth at 20–30 °C, pH 6.0–7.0 and 1–2 % NaCl. Strain XAAS-72T is closely related to members of the genus Pontibacter , namely Pontibacter korlensis CCTCC AB 206081T (97.6%) and Pontibacter flavimaris ACCC 19859T (97.2 %), and <94.6 % related to other currently described Pontibacter strains. The average nucleotide identity values between XAAS-72T and P. korlensis CCTCC AB 206081T and P. flavimaris ACCC 19859T were 77.9 and 86.9 %, respectively; the corresponding digital DNA–DNA hybridization values were 21.7 and 31.8 %. Menaquinone-7 was the predominant respiratory menaquinone. The polar lipids consisted of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified glycolipids and five unidentified lipids. The major cellular fatty acids were summed feature 4 (containing iso-C17 : 1 I/anteiso-C17 : 1 B), summed feature 3 (containing C16 : 1 ω7c/C16 : 1 ω6c) and iso-C15 : 0. The genome length of strain XAAS-72T was 5 054 860 bp with a genomic DNA G+C content of 54.5 mol%. The phenotypic and genotypic data suggest that strain XAAS-72T represents a novel species of the genus Pontibacter , for which the name Pontibacter kalidii sp. nov. is proposed. The strain is XAAS-72T (CGMCC 16594T=KCTC 72095T).

show abstract

Pontibacter beigongshangensis sp. nov., Isolated from the Mash of Wine

Cited by 12 publications

References 19 publications

Culturing Bacteria From Fermentation Pit Muds of Baijiu With Culturomics and Amplicon-Based Metagenomic Approaches

Culturing Bacteria From Fermentation Pit Muds of Baijiu With Culturomics and Amplicon-Based Metagenomic Approaches

Pontibacter liquoris sp. nov. and Pontibacter vulgaris sp. nov., two novel bacteria isolated from the pit mud of Chinese liquor

Pontibacter kalidii sp. nov., isolated from rhizosphere soil of Kalidium foliatum

Contact Info

Product

Resources

About