Pontibacterium granulatum gen. nov., sp. nov., isolated from a tidal flat

Abstract

A Gram-stain-negative, strictly aerobic, moderately halophilic bacterium, designated A-1, was isolated from a tidal flat of the Taean coast in South Korea. Cells were motile rods with a single flagellum showing oxidase-negative and catalase-positive activities and contained poly-β-hydroxyalkanoic acid granules. Growth of strain A-1 was observed at 20-40 °C (optimum, 30 °C), pH 6.0-10.5 (optimum, pH 7.0) and in the presence of 1.0-6.0 % (w/v) NaCl (optimum, 2.0 %). Strain A-1 contained C16 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c) as the major fatty acids. The major polar lipids of strain A-1 were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The isoprenoid quinones detected were ubiquinone-7 and ubiquinone-8. The G+C content of the genomic DNA was 51.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A-1 formed a distinct phylogenetic lineage from other genera within the family Oceanospirillaceae. Strain A-1 shared low 16S rRNA gene sequence similarities with other taxa (≤94.9 %). On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that strain A-1 represents a novel genus and species of the family Oceanospirillaceae, for which the name Pontibacterium granulatum gen. nov., sp. nov. is proposed. The type strain is A-1 (=KACC 18119=JCM 30136).

Taxonomic revision of the genus Amphritea supported by genomic and in silico chemotaxonomic analyses, and the proposal of Aliamphritea gen. nov.

“…nov., a novel species in the genus Aliamphritea . Phenotypic characteristics of the genus Aliamphritea was compared to other Oceanospirillaceae phenotype results [ 33 , 34 ], diagnostic feature’s (morphology, flagellar arrangement and growth at 4°C) differences from other genera were found ( Table 2 ). To elucidate uniqueness of genome backbone of the genus Aliamphritea , we further performed genome comparisons, which could also support the proposal of Al .…”

“… Original data from [ 33 , 34 ]. Phenotypic data for Aliamphritea and Amphritea are from this study and [ 3 – 6 ].…”

“…nov., a novel species in the genus Aliamphritea. Phenotypic characteristics of the genus Aliamphritea was compared to other Oceanospirillaceae phenotype results [33,34], diagnostic feature's (morphology, flagellar arrangement and growth at 4˚C) differences from other genera were found (Table 2). To elucidate…”

Parathalassolituus penaei gen. nov., sp. nov., a novel member of the family Oceanospirillaceae isolated from a coastal shrimp pond in Guangxi, PR China

“…The family Oceanospirillaceae is a member of the order Oceanospirillales , class Gammaproteobacteria , phylum Pseudomonaeota. This family consists of 26 genera, namely Amphritea [2], Aliamphritea [3], Bacterioplanes [4], Bacterioplanoides [5], Balneatrix [6] , Bermanella [7], Corallomonas [8], Litoribacillus [9], Litoribrevibacter [10], Marinomonas [11], Marinospirillum [12], Motiliproteus [13], Neptuniibacter [14], Neptunomonas [15], Nitrincola [16], Oceaniserpentilla [17], Oceanobacter [18], Oceanospirillum [18], Oleibacter [19], Oleispira [20], Pontibacterium [21], Pseudospirillum [18], Spongiispira [22], Reinekea [23], Thalassolituus [24] and Venatorbacter [25]. Except for …”

Pontibacterium sinense sp. nov., a nitrate-reducing and thiosulphate-oxidizing bacterium, isolated from coastal sediment

“…Most genera of the family Oceanospirillaceae were isolated from marine environments, and the strains were salt-tolerant or halophilic. The genus Pontibacterium was proposed by Hyeon et al [1]. At present, this genus contains one species, Pontibacterium granulatum JCM 30316 T , isolated from a tidal flat.…”