Pool–dam structure based microfluidic devices for filtering tumor cells from blood mixtures

Chen, Zaozao; Zhang, Shengyou; Tang, Zuming; Xiao, Pengfeng; Guo, Xiaoying; Zhang, Lu

doi:10.1002/sia.2344

Cited by 22 publications

(14 citation statements)

References 16 publications

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“…Previous studies have proved that the sizes of RBCs and WBCs are smaller than those of tumor cells [32,33]. Moreover, tumor cells not only have a larger volume and cellular karyon but also low deformability [28]. Therefore, the separation of tumor cells from a cell mixture is realizable.…”

Section: Introductionmentioning

confidence: 98%

“…Additionally, the introduction of integrated microfluidics facilitates more complex biological manipulations in one single device [22]. All these advancements have allowed the miniaturization of filtration devices and the realization of precise channel geometries, leading to the more sophisticated separation of cells in microfluidics, such as the isolation of red blood cells (RBCs) and leukocytes [24][25][26], embryos [27], and cancer cells [28,29].…”

Section: Introductionmentioning

confidence: 99%

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Lectin‐aided separation of circulating tumor cells and assay of their response to an anticancer drug in an integrated microfluidic device

Liu

Wang

et al. 2010

Electrophoresis

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Metastasis caused by the entry of circulating tumor cells (CTCs) into the bloodstream or lymphatic vessels is a major factor contributing to death in cancer patients. Separation of CTCs and studies on CTC-drug interactions are very important for prognostic and therapeutic implications of metastatic cancer. In this study, an integrated microfluidic device for CTC separation through the combination of lectin and microstructure is presented. This microfluidic device and lectin concanavalin A were utilized for the separation of K562 cells in whole blood samples. The results showed that the separation efficiency can reach 84%, which is much higher than that of an experiment without concanavalin A treatment. To further demonstrate the feasibility of this microfluidic device application in sequential studies after target cells were separated, the interactions of K562 cells and an anticancer drug, cytarabine, were also examined. After 6 h on-chip treatment with cytarabine, the viabilities of K562 cells were 85.29, 77.05, and 40% for drug concentration levels of 0.25, 0.5, and 1.0 g/L, respectively. This system can facilitate the rapid and efficient in vitro investigation of CTC separation and CTC-related studies.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Lectin‐aided separation of circulating tumor cells and assay of their response to an anticancer drug in an integrated microfluidic device

Liu

Wang

et al. 2010

Electrophoresis

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“…The testing was performed using whole human blood diluted in EMEM medium and spiked with cultured neuroblastoma cells, but the author did not report on trapping efficiency. In 2006, Chen et al, 28 proposed a microfluidic "pool-dam" structure. A series of pools were connected in series with a dam structure, and larger cells were captured in the pools as soon as the cell suspension flowed through the filter.…”

Section: A Separation Based On Mechanical Properties Of Ctcsmentioning

confidence: 99%

Label-free isolation of circulating tumor cells in microfluidic devices: Current research and perspectives

et al. 2013

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This review will cover the recent advances in label-free approaches to isolate and manipulate circulating tumor cells (CTCs). In essence, label-free approaches do not rely on antibodies or biological markers for labeling the cells of interest, but enrich them using the differential physical properties intrinsic to cancer and blood cells. We will discuss technologies that isolate cells based on their biomechanical and electrical properties. Label-free approaches to analyze CTCs have been recently invoked as a valid alternative to "marker-based" techniques, because classical epithelial and tumor markers are lost on some CTC populations and there is no comprehensive phenotypic definition for CTCs. We will highlight the advantages and drawbacks of these technologies and the status on their implementation in the clinics.

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“…In addition, the requirement of external forces, on-chip integration of interfacing components, such as mechanical moving parts, electrodes, or heaters often complicates fabrication procedures and increases the complexity of resulting devices. Passive continuous-flow techniques based on filtering through sieving structures [5][6][7] or by differential interaction of particles with local flow profiles [8][9][10][11] (such as pinch flow fractionation, deterministic lateral displacement) have proven to be relatively simple to operate. However, the need for narrow channel geometries makes these systems less versatile and may lead to channel clogging and particle-particle interactions.…”

Section: Introductionmentioning

confidence: 99%

Dean flow-coupled inertial focusing in curved channels

et al. 2014

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Passive particle focusing based on inertial microfluidics was recently introduced as a high-throughput alternative to active focusing methods that require an external force field to manipulate particles. In inertial microfluidics, dominant inertial forces cause particles to move across streamlines and occupy equilibrium positions along the faces of walls in flows through straight micro channels. In this study, we systematically analyzed the addition of secondary Dean forces by introducing curvature and show how randomly distributed particles entering a simple u-shaped curved channel are focused to a fixed lateral position exiting the curvature. We found the lateral particle focusing position to be fixed and largely independent of radius of curvature and whether particles entering the curvature are pre-focused (at equilibrium) or randomly distributed. Unlike focusing in straight channels, where focusing typically is limited to channel cross-sections in the range of particle size to create single focusing point, we report here particle focusing in a large cross-section area (channel aspect ratio 1:10). Furthermore, we describe a simple u-shaped curved channel, with single inlet and four outlets, for filtration applications. We demonstrate continuous focusing and filtration of 10 lm particles (with >90% filtration efficiency) from a suspension mixture at throughputs several orders of magnitude higher than flow through straight channels (volume flow rate of 4.25 ml/min). Finally, as an example of high throughput cell processing application, white blood cells were continuously processed with a filtration efficiency of 78% with maintained high viability. We expect the study will aid in the fundamental understanding of flow through curved channels and open the door for the development of a whole set of bio-analytical applications. V C 2014 AIP Publishing LLC.

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Pool–dam structure based microfluidic devices for filtering tumor cells from blood mixtures

Cited by 22 publications

References 16 publications

Lectin‐aided separation of circulating tumor cells and assay of their response to an anticancer drug in an integrated microfluidic device

Lectin‐aided separation of circulating tumor cells and assay of their response to an anticancer drug in an integrated microfluidic device

Label-free isolation of circulating tumor cells in microfluidic devices: Current research and perspectives

Dean flow-coupled inertial focusing in curved channels

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