Pool deconvolution approach for high-throughput gene mining from Bacillus thuringiensis

Panwar, Bhupendra Singh; Ram, Chet; R, Narula; Kaur, Sarvjeet

doi:10.1007/s00253-017-8633-6

Cited by 11 publications

(4 citation statements)

References 82 publications

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“…Bacillus thuringiensis ( Bt ) is a Gram-positive soil bacterium expressing insecticidal crystalline proteins (ICPs) that are exceptionally toxic to specific classes of pests ( Panwar et al, 2018 ). Insecticidal activity in insect-resistant Bt crops is expressed by the genes coding for parasporal crystal protoxins ( Palma et al, 2014 ).…”

Section: Biotechnological Approaches In Insect Pest Managementmentioning

confidence: 99%

Biotechnological Approaches for Host Plant Resistance to Insect Pests

et al. 2022

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Annually, the cost of insect pest control in agriculture crosses billions of dollars around the world. Until recently, broad-spectrum synthetic pesticides were considered as the most effective means of pest control in agriculture. However, over the years, the overreliance on pesticides has caused adverse effects on beneficial insects, human health and the environment, and has led to the development of pesticide resistant insects. There is a critical need for the development of alternative pest management strategies aiming for minimum use of pesticides and conservation of natural enemies for maintaining the ecological balance of the environment. Host plant resistance plays a vital role in integrated pest management but the development of insect-resistant varieties through conventional ways of host plant resistance takes time, and is challenging as it involves many quantitative traits positioned at various loci. Biotechnological approaches such as gene editing, gene transformation, marker-assisted selection etc. in this direction have recently opened up a new era of insect control options. These could contribute towards about exploring a much wider array of novel insecticidal genes that would otherwise be beyond the scope of conventional breeding. Biotechnological interventions can alter the gene expression level and pattern as well as the development of transgenic varieties with insecticidal genes and can improve pest management by providing access to novel molecules. This review will discuss the emerging biotechnological tools available to develop insect-resistant engineered crop genotypes with a better ability to resist the attack of insect pests.

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Section: Biotechnological Approaches In Insect Pest Managementmentioning

confidence: 99%

Biotechnological Approaches for Host Plant Resistance to Insect Pests

et al. 2022

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“…48 There exists a large variety of insects which are not considered IPs neither VHDs, 18,22,23,30,41,49 Biopesticides and anti-pest crops have been developed by genetically modifying the crops with Bt genes, resulting in thousands of Bt strains isolated and studied. 4,20,21,[50][51][52][53][54] However, the evolution of resistance of pests to the insecticidal proteins from Bt produced by transgenic crops is drastically reducing the effectiveness of this method. 50,[55][56][57][58][59][60][61] Indeed, in 2005 was reported that 1 out of 13 insect species were vulnerable to transgenic crops, whereas eight years later, 5 out of 13 had developed resistance to Bt modified crops.…”

Section: Mode Of Action Of Bt Crystals On Insect Pestsmentioning

confidence: 99%

Bacillus thuringiensis (Bt) is more than a special agent for biological control of pests

Sanchez–Yáñez¹,

Rico²,

Ulíbrri³

2022

JABB

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Bacillus thuringiensis (Bt) is a bacterial genus known mainly for its capability to synthesize, in addition to spores, a parasporal body or crystal (δ––endotoxin), comprised of proteins which are toxic to pest insects, i.e., Coleoptera, Diptera and Lepidoptera, and other orders. As well as plant pathogenic nematode and many other applications as endophytic plant growth promoting bacteria, or cleaning enviornmental form some chemicals polluting agents, and even in human medicine for cancer prevention. The Bt coexists in a close relationship with insects to which eventually infects and poisons. It is likely that these unfavorable changes occurred during the evolutionary pathway in the interactions Bacillus–insect, which resulted in the present biochemical diversity between these species. The goal of this minireview is to show role of Bt in comparation with other genus Bacillus and explain why Bt is of interest for biological control of pests in conventional, protected and organic agriculture regarding other biotechnological applications to fully exploit the beneficial potential of Bt

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“…Despite the success of the application of Bt crystal proteins for the biological control of pests, at present it is still necessary to identify new Cry toxins with greater toxicity; this approach is considered one of the best ways to counter the potential resistance evolved by insects as well as in developing products against a wider spectrum of insect pests. Traditionally, Bt isolates were screened for their insecticidal spectrum by the time-consuming and laborious insect bioassays [22,46]. Since only a limited number of cry genes have been used for insect control either in sprays or transgenic crops so far, novel insecticidal genes are required [31].…”

Section: New Cry Toxinsmentioning

confidence: 99%

“…The emergence of resistant insects is a problem that both Bt sprays and plant products are likely to face in the future [32]. Several strategies, such as the use of spatial or temporal refugia, high or ultrahigh doses, and gene pyramiding to express two toxins, or two insect control approaches, such that the possibility of evolution of resistance to two toxins/approaches, independent of each other, is greatly diminished, can be a promising approach to prolong the efficacy of products based on Bt [36,46].…”

Section: Resistance To Bacillus Thuringiensismentioning

confidence: 99%

Toxic Potential ofBacillus thuringiensis: An Overview

Fernández-Chapa¹,

Ramírez-Villalobos²,

Galán-Wong³

2019

Protecting Rice Grains in the Post-Genomic Era

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The toxins of Bacillus thuringiensis (Bt) have shown great potential in the control of harmful insects affecting human health and agriculture, used as the main biological agent for the formulation of bioinsecticides due to its specificity to target different insects' orders. This has led Bt-based products to become the best-selling biological insecticides in the world since the genes encoding insecticidal proteins have been successfully used in novel insecticidal formulation, genetically engineered (GE) crops, and development of transgenic rice that produce insecticidal toxins derived from Bacillus thuringiensis. It has been proven that insecticidal activity of Bt protein crystals can prolong their toxicity in shelf life or field under specific conditions, and this can improve the use of special strains and formulations to control insect vectors of diseases. Bt toxins have shown well-documented toxicity against lepidopterans, coleopterans, hemipterans, dipterans, nematodes, Rhabditida and human cancer cells of various origins. These crystal toxins may be responsible for other novel biological properties suggesting a pluripotential nature with different specificities.

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Pool deconvolution approach for high-throughput gene mining from Bacillus thuringiensis

Cited by 11 publications

References 82 publications

Biotechnological Approaches for Host Plant Resistance to Insect Pests

Biotechnological Approaches for Host Plant Resistance to Insect Pests

Bacillus thuringiensis (Bt) is more than a special agent for biological control of pests

Toxic Potential ofBacillus thuringiensis: An Overview

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