Poor association between allergen‐specific serum immunoglobulin E levels, skin sensitivity and basophil degranulation: a study with recombinant birch pollen allergen Bet v 1 and an immunoglobulin E detection system measuring immunoglobulin E capable of binding to FcɛRI

Purohit, Ashok; Laffer, Sylvia; Metz-Favre, C.; Vérot, A.; Kricek, Franz; Valenta, Rudolf; Pauli, G.

doi:10.1111/j.1365-2222.2005.02156.x

Cited by 42 publications

(38 citation statements)

References 28 publications

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“…Some papers reported that antigen-specific IgE levels were poorly associated with skin sensitivity and basophil degranulation (Conroy et al, 1977;Dembo and Goldstein, 1978;Malveaux et al, 1978;Purohit et al, 2005), and mast cells were activated by IgE-independent pathways (Tatemoto et al, 2006), these results support current results.…”

Section: Discussionsupporting

confidence: 82%

Enhancement of immediate allergic reactions by trichloroethylene ingestion via drinking water in mice

et al. 2010

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-The prevalence of allergic disorders is increasing in industrial areas and countries. Recent reports suggest that some environmental pollutants are related to the increase in allergic diseases, and we reported that trichloroethylene (TCE) is a candidate chemical for causing the increase of allergic diseases, as TCE ingestion is associated with allergic reaction enhancement. TCE is widely used in many industries, and it is commonly detected as an environmental contaminant. This study aimed to clarify the immunotoxicity of TCE in detail. BALB/c mice were treated with TCE dissolved in drinking water for 2 and 4 weeks, and the mice were immunized with ovalbumin (OVA)/aluminum hydroxide (alum) twice. On the final day of the TCE exposure period, we measured the active cutaneous anaphylaxis (ACA) reaction and the antigen-specific IgE level in serum as well as the histamine level at the allergic reaction site and assayed the proliferation rates of splenocytes collected from the animals. The ACA reaction was enhanced by TCE ingestion. The OVA specific IgE level in mice was enhanced by TCE exposure for 4 weeks. The proliferation rate of the splenocytes was enhanced by TCE ingestion for 2 and 4 weeks. The enhancement of the ACA reaction by TCE ingestion via drinking water may be related to the increase in splenocyte proliferation. On the other hand, it may be weakly related to antigen-specific IgE production.

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Section: Discussionsupporting

confidence: 82%

Enhancement of immediate allergic reactions by trichloroethylene ingestion via drinking water in mice

et al. 2010

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“…This result disagrees with the study of Purohit et al [12] who showed that the concentration of allergen-specific IgE antibodies capable of binding to FcεRI and biological sensitivities were not necessarily associated. However, it is in agreement with the study of McDermott et al [46] who stated that the ability of Ara h 2 to effectively cross-link IgE from a specific serum was related to the proportion of anti-Ara h 2 antibodies in that serum.…”

Section: Discussioncontrasting

confidence: 57%

“…By ELISA, one epitope is sufficient to obtain reactivity, and thus this could lead to false-positive results in the identification of relevant allergens that are biologically active. Additionally, parameters such as the ability of the IgE to bind its receptor or the binding affinity are not taken into account by ELISA [11, 12]. …”

Section: Introductionmentioning

confidence: 99%

Evaluation of an in vitro Mast Cell Degranulation Test in the Context of Food Allergy to Wheat

Bodinier

Brossard

Triballeau

et al. 2008

Int Arch Allergy Immunol

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Background: Antigenic profiles obtained by ELISA with IgE from patients with wheat food allergy (WFA) established that major allergens are albumins/globulins (AG) for children suffering from atopic eczema/dermatitis syndrome (AEDS), ω5-gliadins for adults suffering from wheat-dependent exercise-induced anaphylaxis (WDEIA), anaphylaxis or urticaria and low-molecular-weight (LMW) glutenin subunits for patients with anaphylaxis. We aimed to characterize a new mast cell transfectant for its ability to degranulate with wheat proteins and patient sera and compare these results to those obtained by ELISA. Methods: Thirty sera from patients with WFA were tested: 14 with AEDS (group 1) and 16 with WDEIA, anaphylaxis or urticaria (group 2). An IgE Fc receptor (FcΕRI) humanized rat RBL-2H3 line was established by transfection with cDNAs encoding α-, β- and γ-subunits for the human IgE receptor. Results: A humanized RBL clone was selected for its capacity to express mRNA α-, β- and γ-subunits of FcΕRI, to bind allergen-specific human IgE and to degranulate. In group 1, sera induced enhanced degranulation with AG extract, but rarely reacted with gliadins and glutenins. In group 2, half of the sera showed degranulation with LMW glutenins whereas the AG fraction and lipid transfer proteins were rarely positive. ω5-Gliadins did not appear as a major allergen in degranulation assays, although functional allergen-specific IgE was measurable in appreciable amounts. Conclusion: Our data demonstrate that in wheat food allergen evaluation, correlation exists between mast cell degranulation and IgE measurements, depending on the type of allergen. Therefore, the biological activity of some allergen types may also be affected by other parameters.

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“…For a few sera, the antibody levels and biological activity were not associated (i.e. A2, A7, A29), but this phenomenon has already been described for several allergens including Der p 1 and Der p 2 [7,35,36]. Several factors such as the number and geometry of IgE epitopes as well as affinity/avidity of IgE binding may influence the mediator release activity.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Der p 10: a diagnostic marker for broad sensitization in house dust mite allergy

Resch¹,

Weghofer²,

Seiberler³

et al. 2011

Clin Experimental Allergy

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Poor association between allergen‐specific serum immunoglobulin E levels, skin sensitivity and basophil degranulation: a study with recombinant birch pollen allergen Bet v 1 and an immunoglobulin E detection system measuring immunoglobulin E capable of binding to FcɛRI

Cited by 42 publications

References 28 publications

Enhancement of immediate allergic reactions by trichloroethylene ingestion via drinking water in mice

Enhancement of immediate allergic reactions by trichloroethylene ingestion via drinking water in mice

Evaluation of an in vitro Mast Cell Degranulation Test in the Context of Food Allergy to Wheat

Molecular characterization of Der p 10: a diagnostic marker for broad sensitization in house dust mite allergy

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