Poor prognosis osteosarcoma: new therapeutic approach

Biasin, Eleonora; Mereuta, Oana Madalina; Muraro, Michela; Luksch, Roberto; Ferrari, Stefano; Aglietta, Massimo; Madon, E

doi:10.1038/bmt.2008.71

Cited by 29 publications

(27 citation statements)

References 28 publications

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“…In localized osteosarcoma patients, the five-year survival rates are approximately 65%-75% (Mirabello et al, 2009). However, the prognosis for patients with recurrence and metastases is quite poor (Fagioli et al, 2008). Additionally, most of the neoadjuvant chemotherapy drugs have extensive side effects, and multidrug resistant cases are common, particularly with cisplatin and doxorubicin (Longhi et al, 2000;Chou and Gorlick, 2006;Schwartz et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Artesunate inhibits growth and induces apoptosis in human osteosarcoma HOS cell line in vitro and in vivo

Peng

et al. 2011

J. Zhejiang Univ. Sci. B

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This paper aims to investigate the effects of artesunate (ART) on growth and apoptosis in human osteosarcoma HOS cell line in vitro and in vivo and to explore the possible underlying mechanisms. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The induction of apoptosis was detected by light and transmission electron microscopy and flow cytometry. Western blot analysis was used to investigate the related mechanisms. Nude mice were further employed to investigate the antitumour activity of ART in vivo. MTT assay results demonstrated that ART selectively inhibits the growth of HOS cells in a dose-and time-dependent manner. Based on the findings of light and transmission electron microscopy, Hoechst 33258 staining, and fluorescein isothiocyanate (FITC)-annexin V staining, the cytotoxicity of ART in HOS cells occurs through apoptosis. With ART treatment, cytosolic cytochrome c was increased, Bax expression was gradually upregulated, Bcl-2 expression was downregulated, and caspase-9 and caspase-3 were activated. Thus, the intrinsic apoptotic pathway may be involved in ART-induced apoptosis. Cell cycle analysis by flow cytometry indicated that ART may induce cell cycle arrest at G 2 /M phase. In nude mice bearing HOS xenograft tumours, ART inhibited tumour growth and regulated the expressions of cleaved caspase-3 and survivin, in agreement with in vitro observations. ART has a selective antitumour activity against human osteosarcoma HOS cells, which may be related to its effects on induction of apoptosis via the intrinsic pathway. The results suggest that ART is a promising candidate for the treatment of osteosarcoma.

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Section: Introductionmentioning

confidence: 99%

Artesunate inhibits growth and induces apoptosis in human osteosarcoma HOS cell line in vitro and in vivo

Peng

et al. 2011

J. Zhejiang Univ. Sci. B

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“…Since osteosarcoma is highly aggressive and commonly metastasizes to the lung, patients with metastatic or recurrent osteosarcoma usually have an extremely poor prognosis (2)(3)(4)(5). Although the diagnosis and treatment of osteosarcoma have been improved significantly during the past 30 years (3,6), approximately 30-40% of patients experience osteosarcoma relapse, mostly within the first three years after diagnosis. Common factors, such as demographics (age and gender), tumor size, site and stage have been used for the prognosis of advanced osteosarcoma, but the results are difficult to reproduce due to the usage of various techniques and units for measurement.…”

Section: Introductionmentioning

confidence: 99%

“…Common factors, such as demographics (age and gender), tumor size, site and stage have been used for the prognosis of advanced osteosarcoma, but the results are difficult to reproduce due to the usage of various techniques and units for measurement. Furthermore, tumor-related metastasis and chemotherapeutic response are significant prognostic factors, but they usually occur at the late stage of osteosarcoma (1)(2)(3)(4). Therefore, there is an urgent need for the discovery of new reliable and efficient biomarkers for the prognosis of osteosarcoma.…”

Section: Introductionmentioning

confidence: 99%

Expression and clinical significance of extracellular matrix metalloproteinase inducer, EMMPRIN/CD147, in human osteosarcoma

Lü

Kim

et al. 2012

Oncology Letters

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Abstract. Osteosarcoma is the most common primary malignant bone tumor in children and adolescents. Recent studies have shown that extracellular matrix metalloproteinase inducer (EMMPRIN/CD147) promotes adhesion, invasion and metastasis of malignant tumor cells. The aim of this study was to investigate the impact of EMMPRIN/CD147 expression on prognosis and its correlation with clinicopathological characteristics in patients with osteosarcoma. The expression of EMMPRIN/CD147 in 55 surgical specimens from patients with osteosarcoma at stage IIA or above, 15 non-tumor rib bone tissues, three human osteosarcoma cell lines (Saos-2, U-2OS and MG-63), the human osteoblast cell line HOB and the malignant melanoma cell line A375 were examined by immunohistochemistry, western blot analysis and ELISA, respectively. The potential association of the levels of EMMPRIN/CD147 expression in osteosarcoma specimens with the overall survival of patients was statistically analyzed. We found that the EMMPRIN/CD147 was expressed in 45 out of 55 osteosarcomas, with immunoreactivity primarily within the membrane and cytoplasm of tumor cells, but not in the non-tumor bone tissues. We also observed that EMMPRIN/CD147 was expressed in Saos-2, U-2OS, MG-63 and A375, but not in HOB cells. The levels of EMMPRIN/CD147 expression correlated positively with the pathological degree of osteosarcoma and negatively with the survival period of patients with osteosarcoma. The expression of EMMPRIN/CD147 is a potential factor in the development and prognosis of osteosarcoma and may be a novel therapeutic target of human osteosarcoma.

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“…Osteosarcoma, a malignant tumor arising from primitive transformed cells of mesenchymal origin that exhibit osteoblastic differentiation and produce malignant osteoid, is difficult to completely remove by radical (Fagioli et al, 2008). The residual local tumor cells leave patients vulnerable to local recurrence and poor clinical outcomes.…”

Section: Introductionmentioning

confidence: 99%

Benzochloroporphyrin Derivative Induced Cytotoxicity and Inhibition of Tumor Recurrence During Photodynamic Therapy for Osteosarcoma

Gong

Sun²,

Hu³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Photodynamic therapy (PDT) is a promising cancer treatment modality that uses dye-sensitized photooxidation of biologic matter in target tissue. This study explored effects of the photosensitizer BCPD-17 during PDT for osteosarcoma. LM-8 osteosarcoma cells were treated with BCPD-17 and cell viability after laser irradiation was assessed in vitro with the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. The effects of BCPD-17 during PDT recurrence were then examined on tumor-bearing mice in vivo. BCPD-17 had dosedependent cytotoxic effects on LM-8 osteosarcoma cells after laser irradiation which also had energy-dependent effects on the cells. The rate of local recurrence was reduced when marginal resection of mice tumors was followed by BCPD-17-mediated PDT. Our results indicated BCPD-17-mediated PDT in combination with marginal resection of tumors is a potentially new effective treatment for osteosarcoma.

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Poor prognosis osteosarcoma: new therapeutic approach

Cited by 29 publications

References 28 publications

Artesunate inhibits growth and induces apoptosis in human osteosarcoma HOS cell line in vitro and in vivo

Artesunate inhibits growth and induces apoptosis in human osteosarcoma HOS cell line in vitro and in vivo

Expression and clinical significance of extracellular matrix metalloproteinase inducer, EMMPRIN/CD147, in human osteosarcoma

Benzochloroporphyrin Derivative Induced Cytotoxicity and Inhibition of Tumor Recurrence During Photodynamic Therapy for Osteosarcoma

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