In this work, the population dynamics and reproductive aspects of two sympatric Hyalella species in a river spring were analyzed in the northwestern state of Rio Grande do Sul, Brazil. The samplings were performed by one person, for ten minutes and using a hand net for one-year period (March 2018 to April 2019) and transported to the laboratory. All individuals sampled were sexed, measured, and separated by cephalothorax length (CC) size classes. Hyalella gauchensis Streck & Castiglioni, 2017 was 22.7 times more frequent and showed significantly greater mean cephalothorax when compared to Hyalella longipropodus Limberger, Graichen & Castiglioni, 2021. Males had a larger body size (CC) than females, although they occur in smaller numbers in both species. The total frequency distribution in CC size classes was bimodal in males and juveniles, and polimodal in females of H. gauchensis. In H. longipropodus the distribution as bimodal in males, females and juveniles. Both H. gauchensis and H. longipropodus had their breeding season and recruitment during the colder seasons of the year (fall and winter, respectively). However, there was a seasonal temporal separation of the intensity peaks between the two species, which could indicate different strategies and/or evolutionary adaptations for their coexistence.