Temporal variation of insect communities in urban environments is poorly known and mechanisms driving these changes are unclear, as are the implications for insectivorous predators. We examined the relationships between season and nocturnal aerial insect biomass and biodiversity, and between temperature and insect biomass in the Adelaide zoological gardens from December 2005 to September 2006. We also compared the effectiveness of two insect trap types and used a bat detector to assess bat activity in relation to insect biomass. During the study, 9,939 insects from 13 orders were collected at the Adelaide zoo with a Malaise trap and a light trap. Mass and diversity of insects were highest during warm months, as was bat activity, and bat activity was positively correlated with insect biomass. Winter-active insects consisted predominantly of Diptera and Lepidoptera, which may provide an important winter food resource for insectivorous bats. The Malaise trap attracted fewer insect orders and biomass than did the light trap, and insects congregated within 6 m of artificial lights, so bats that forage at lights may have an advantage in urban areas. A strong need for the inclusion of urban insects to biodiversity inventories exists in the context of bat conservation.