Population diversity of Listeria monocytogenes in quargel (acid curd cheese) lots recalled during the multinational listeriosis outbreak 2009/2010

Schoder, Dagmar; Stessl, Beatrix; Szakmary-Brändle, Kati; Rossmanith, Peter; Wagner, Martin

doi:10.1016/j.fm.2013.11.006

Cited by 24 publications

(11 citation statements)

References 29 publications

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“…Recent studies analysing the L. monocytogenes strains isolated from the Quargel lots from the Austrian producer during the last period of the outbreak (December 2009 to January 2010) revealed that all strains isolated belong to either of the two PFGE types and showed the same PFGE patterns as the human outbreak isolates. PFGE typing revealed that PFGE type 2 (the PFGE type of QOC2) was highly dominant among the food isolates from this period [69]. Similarly, QOC2 (PFGE type2) was also predominant among the human isolates in this time period [17].…”

Section: Resultsmentioning

confidence: 99%

“…Two different types of Quargel were produced in this particular plant: either red-smear-ripened, or mold-ripened. Interestingly, only the red-smear Quargel lots were L. monocytogenes positive [14], [69]. Furthermore, the production lines for both Quargel types were identical except for the smearing process, which was performed in different machines in physically separated areas of the production plant [14].…”

Section: Resultsmentioning

confidence: 99%

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Genome Sequencing of Listeria monocytogenes “Quargel” Listeriosis Outbreak Strains Reveals Two Different Strains with Distinct In Vitro Virulence Potential

et al. 2014

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A large listeriosis outbreak occurred in Austria, Germany and the Czech Republic in 2009 and 2010. The outbreak was traced back to a traditional Austrian curd cheese called “Quargel” which was contaminated with two distinct serovar 1/2a Listeria monocytogenes strains (QOC1 and QOC2). In this study we sequenced and analysed the genomes of both outbreak strains in order to investigate the extent of genetic diversity between the two strains belonging to MLST sequence types 398 (QOC2) and 403 (QOC1). Both genomes are highly similar, but also display distinct properties: The QOC1 genome is approximately 74 kbp larger than the QOC2 genome. In addition, the strains harbour 93 (QOC1) and 45 (QOC2) genes encoding strain-specific proteins. A 21 kbp region showing highest similarity to plasmid pLMIV encoding three putative internalins is integrated in the QOC1 genome. In contrast to QOC1, strain QOC2 harbours a vip homologue, which encodes a LPXTG surface protein involved in cell invasion. In accordance, in vitro virulence assays revealed distinct differences in invasion efficiency and intracellular proliferation within different cell types. The higher virulence potential of QOC1 in non-phagocytic cells may be explained by the presence of additional internalins in the pLMIV-like region, whereas the higher invasion capability of QOC2 into phagocytic cells may be due to the presence of a vip homologue. In addition, both strains show differences in stress-related gene content. Strain QOC1 encodes a so-called stress survival islet 1, whereas strain QOC2 harbours a homologue of the uncharacterized LMOf2365_0481 gene. Consistently, QOC1 shows higher resistance to acidic, alkaline and gastric stress. In conclusion, our results show that strain QOC1 and QOC2 are distinct and did not recently evolve from a common ancestor.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Genome Sequencing of Listeria monocytogenes “Quargel” Listeriosis Outbreak Strains Reveals Two Different Strains with Distinct In Vitro Virulence Potential

et al. 2014

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“…In 2009 a listeriosis outbreak occurred in Austria and Germany caused by contaminated acid curd cheese (Fretz et al, 2010;Schoder, Stessl, Szakmary-Brandle, Rossmanith, & Wagner, 2014). Since that time awareness of the danger in dairies has risen and greater importance has been attached to the prevention of cheese and production plant contamination through controls and monitoring, including cleansing and disinfection steps.…”

Section: Introductionmentioning

confidence: 99%

Screening and characterisation of bacteriophage P100 insensitive Listeria monocytogenes isolates in Austrian dairy plants

et al. 2016

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“…PFGE was used for the molecular subtyping of Listeria monocytogenes isolates in a commercial cheese made from pasteurized milk that caused an outbreak in Germany from October 2006 through February 2007 (Koch et al, 2010) and in Quebec, Canada, in 2008(Gaulin et al, 2012. Additionally, in 2010, this technique was useful in determining the genotypic diversity of Listeria monocytogenes in the acid curd cheese that caused a multinational outbreak between 2009 and 2010 (Schoder et al, 2014).…”

Section: Pulsed Field Gel Electrophoresismentioning

confidence: 99%

Foodborne bacteria in dairy products: Detection by molecular techniques

Cancino‐Padilla

Fellenberg

Franco

et al. 2017

Ciencia e investigación agraria

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Staphylococcus aureus, Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 are the most frequent potential pathogens associated with milk or dairy products in industrialized countries and are therefore the main microbiological hazards linked to raw milk and raw cheese. This review summarizes the scientific information about outbreaks related to foodborne pathogens in dairy products and highlights the increasing application of molecular approaches to detect and identify the bacteria responsible for these outbreaks. Molecular techniques have facilitated the rapid detection and identification of foodborne pathogens, which has been crucial for current surveillance and outbreak control.

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Population diversity of Listeria monocytogenes in quargel (acid curd cheese) lots recalled during the multinational listeriosis outbreak 2009/2010

Cited by 24 publications

References 29 publications

Genome Sequencing of Listeria monocytogenes “Quargel” Listeriosis Outbreak Strains Reveals Two Different Strains with Distinct In Vitro Virulence Potential

Genome Sequencing of Listeria monocytogenes “Quargel” Listeriosis Outbreak Strains Reveals Two Different Strains with Distinct In Vitro Virulence Potential

Screening and characterisation of bacteriophage P100 insensitive Listeria monocytogenes isolates in Austrian dairy plants

Foodborne bacteria in dairy products: Detection by molecular techniques

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