Population Dynamics and Nitrite Build-Up in Activated Sludge and Biofilm Processes for Nitrogen Removal

Rols, Jean‐Luc; Mauret, M.; Rahmani, Hossein A.; Nguyen, Kien T.; Capdeville, B.; Cornier, J. C.; Déguin, A.

doi:10.2166/wst.1994.0301

Cited by 15 publications

(21 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Whatever the temperature, Mauret et al (1996) observed a transient nitrite build-up of roughly 50% of the initial ammonia concentration for threshold concentrations of FA of 8.91 mg N L −1 . Villaverde et al (2000) and Rols et al (1994) remark that the inhibition phenomenon is specific and depends both upon the concentration of nitrifying organisms Villaverde et al (1997) as upon the threshold concentration (on volume basis) of FA causing inhibition. In doing so, Rols et al (1994) established the threshold of inhibition of NOB between 0.5 and 3 mg NH 3 -N mg −1 viable NOB biomass.…”

Section: Ammonium (Nh + 4 ) or Free Ammonia (Nh 3 )mentioning

confidence: 99%

“…Villaverde et al (2000) and Rols et al (1994) remark that the inhibition phenomenon is specific and depends both upon the concentration of nitrifying organisms Villaverde et al (1997) as upon the threshold concentration (on volume basis) of FA causing inhibition. In doing so, Rols et al (1994) established the threshold of inhibition of NOB between 0.5 and 3 mg NH 3 -N mg −1 viable NOB biomass. For the same reason, Suthersan and Ganczarczyk (1986) introduce the FA over biomass ratio as a specific inhibition effect measure.…”

Section: Ammonium (Nh + 4 ) or Free Ammonia (Nh 3 )mentioning

confidence: 99%

“…Both AOB and NOB were capable of acclimating to FA levels as high as 40 mg NH 3 -N L −1 (Turk & Mavinic 1989). Also Rols et al (1994) believe that the history of the sludge (in their case enrichment in the presence of high FA concentrations) or the operation of the reactor are factors altering the growth dynamics of NOB towards acclimatisation to NH 3 . Results of experiments with submerged biofilters (Villaverde et al 2000) also suggest that NOB develop the capability to resist higher concentrations of FA after exposure for long periods of time to constant FA concentrations: the threshold value of 0.2 mg NH 3 -N g −1 VAS (Volatile Attached Solids) after 2 months of operation increased to 0.5-0.7 mg NH 3 -N g −1 VAS after 6 months, for constant VAS concentration and NH 3 loads (66.4 mg NH 3 -N d −1 = 2 g NH + 4 -N d −1 ).…”

Section: Ammonium (Nh + 4 ) or Free Ammonia (Nh 3 )mentioning

confidence: 99%

See 2 more Smart Citations

Origin, causes and effects of increased nitrite concentrations in aquatic environments

Philips

Laanbroek

Verstraete

2002

Rev Environ Sci Biotechnol

314

198

View full text Add to dashboard Cite

Literature frequently mentions increased nitrite concentrations along with its inhibitory effect towards bacteria and aquatic life. Nitrite accumulation has been studied for decades, and although numerous causal factors have already been commented on in literature, the mechanism of nitrite accumulation is not always clear. From the broad range of parameters and environmental factors reviewed in this paper, it is obvious that the causes and consequences of nitrite accumulation are not yet completely understood. Among others, pH, dissolved oxygen, volatile fatty acids, phosphate and reactor operation have been found to play a role in nitrite accumulation, which results from differential inhibition or disruption of the linkage of the different steps in both nitrification and denitrification. In the case of nitrification, this differential inhibition could lead to the displacement or unlinking of the ammonia oxidisers and nitrite oxidisers. In this paper, the idea is formulated that the nitrifier population forms a role model for the total microbial community. Increased nitrite concentrations would in this aspect not only signal a disruption of nitrifiers, but possibly also of the total configuration of the microbial community.Abbreviations: AMO -Ammonia mono-oxygenase; Anammox -Anaerobic ammonium oxidation; AOBAmmonia-oxidising bacteria; ATP -Adenosine triphosphate; COD -Chemical oxygen demand; DNRA -Dissimilatory nitrate reduction to ammonium; DO -Dissolved oxygen; EU -European union; FA -Free ammonia; F/M -Food to micro-organism ratio; FNA -Free nitrous acid; HAO -Hydroxylamine oxidoreductase; HRTHydraulic residence time; NDBEPR -Enhanced biological phosphate removal with nitrification and denitrification; NO -Nitric oxide; NOB -Nitrite-oxidising bacteria; NOR -Nitrite oxidoreductase; OLAND -Oxygen limited autotrophic nitrification denitrification; RBC -Rotating biological contactor; Sharon -Single reactor high activity ammonia removal over nitrite; SRT -Sludge residence time; TAN -Total ammoniacal nitrogen; TOC -Total organic carbon; VAS -Volatile attached solids

show abstract

Section: Ammonium (Nh + 4 ) or Free Ammonia (Nh 3 )mentioning

confidence: 99%

Section: Ammonium (Nh + 4 ) or Free Ammonia (Nh 3 )mentioning

confidence: 99%

Section: Ammonium (Nh + 4 ) or Free Ammonia (Nh 3 )mentioning

confidence: 99%

See 1 more Smart Citation

Origin, causes and effects of increased nitrite concentrations in aquatic environments

Philips

Laanbroek

Verstraete

2002

Rev Environ Sci Biotechnol

314

198

View full text Add to dashboard Cite

show abstract

“…Il en est de même pour les caractéristiques de la population nitrifiante, d'après les travaux de BALMELLE et al (1992) et ROLS et al (1994. Ces auteurs ont remarqué l'importance de la composition et de l'histoire de la biomasse nitrifiante : en fonction du groupe de bactéries prédominant dans la culture (population mixte ou enrichie en bactéries nitritantes), des réponses différentes aux paramètres environnementaux sont obtenues, les principaux étant les concentrations en NH 3 et en HN0 2 .…”

Section: Synoptic Représentation Of the Nitrogen Biological Treatmentunclassified

“…Cependant, la cause majeure du déplacement est la composition de la biomasse, laquelle est dans notre cas principalement nitratante. ROLS et al (1994) ont montré qu'une culture enrichie en bactéries nitritantes (et donc appauvrie en bactéries nitratantes) présentait plus de sensibilité à NH 3 qu'une culture considérée comme mixte. Le fait qu'une population enrichie en bactéries nitratantes présente dans notre cas une activité respiratoire résiduelle même à 70 mgN-NH 3 /l reste cohérent avec ces observations.…”

Section: Effet De La Concentration En Nhunclassified

Étude du processus de nitratation avec des boues activées : effet inhibiteur de l'ammoniac sur les bactéries nitratantes

Vazquez-Rodriguez¹,

Rolst²

2005

rseau

View full text Add to dashboard Cite

Cet article porte sur l'étude de la réaction de nitratation (oxydation de nitrites en nitrates) par voie biologique aérobie avec des bactéries autotrophes nitratantes. Les phénomènes d'inhibition de l'ammoniac sur l'activité nitratante de populations microbiennes issues de boues activées (populations mixtes ou enrichies en bactéries nitratantes) ont été caractérisés.A l'aide d'une méthodologie faisant appel à la respirométrie, les caractéristiques de la population enrichie ont été définies :- les conditions optimales de mise en oeuvre sont un pH de 7,8 et une température de 29·C- les paramètres cinétiques définissant les performances sont QSmax=61 mgN-NO2-/gMVS×h et KS=3,04 mgN-NO2-/l- cette population présente une bonne tolérance vis-à-vis de NH3, une inhibition de 60% de la respiration des bactéries nitratantes ayant été obtenue pour 11,4 mgN-NH3/l.Les mêmes effets inhibiteurs ont été observés lors de la mise en culture discontinue de populations mixtes dans les conditions optimales de pH et température. Dans ces conditions de mise en oeuvre, différentes cultures dont les concentrations en biomasse totale ont varié de 0,1 à 2 gMVS/l, en présence de 3 mgN-NH3/l, ont présenté la même vitesse spécifique de nitratation. Ces phénomènes d'inhibition de la nitratation par NH3 paraissent complexes et fortement dépendant de facteurs environnementaux qui agissent sur la dynamique de croissance de ces bactéries.This paper focuses both on nitrification and on inhibition by ammonia of the bacteria responsible for this process in activated sludge (nitrifying bacteria). Nitrification (autotrophic nitrite oxidation to nitrate) is the limiting step in wastewater treatment plants during nitrogen biological treatment (ammonia is known as the major inhibitor). To assess the effects of environmental conditions on nitrification, respirometric measurements of a nitrifying bacteria-enriched activated sludge were carried out using a chemostat providing a stable nitrite-oxidizing population. The influence of pH, temperature, and the free ammonia concentration [NH3-N] was studied. The maximum activity of the nitrifying bacteria was found at pH 7.8 and at 29 oC, with the 90% range of maximum activity lying between pH values of 7.5 and 8.1. Activation and deactivation energies for nitrification were estimated by Arrhenius analysis to be 12.2 and - 41.5 kcal/mol, respectively. A Q10 value (multiplicative factor of oxygen consumption rate for each 10oC increase) of 2.8 was determined. Saturation or half-velocity coefficient (Ks) and maximum specific rate of nitrite oxidation (QSmax) were calculated by respirometric measurements, and are 3.04 mg NO2--N/l and 61 mg NO2--N/g VSS.h, respectively. In the study of effect of free ammonia, respirometric measurements were performed at ammonia concentrations ranging from 0.1 to 70 mg NH3-N/l. For a concentration of 11.4 mg NH3-N/l, a nitrification inhibition degree of 60% was reached. Contrary to the work of Anthonisen et al. (1976), which showed total inhibition of nitrifying bacteria at a concentrat...

show abstract

Partial nitrification—operational parameters and microorganisms involved

Sinha

Annachhatre

2006

Rev Environ Sci Biotechnol

164

View full text Add to dashboard Cite

Nitrite is a common intermediate in at least three different oxidative or reductive biochemical pathways that occur in nature (nitrification, denitrification and dissimilatory or assimilatory nitrate reduction). Nitrite accumulation or partial nitrification has been reported in literature for decades. In engineered systems, partial nitrification is of interest as it offers cost savings in aeration as well as in the form of lesser need for addition of organic carbon as compared to the conventional denitrification. A broad range of operating parameters and factors has been reviewed in this paper which are essential for achieving partial nitrification. Of these, pH, dissolved oxygen (DO), temperature, free ammonia (FA) and nitrous acid concentrations, inhibitory compounds are important factors in achieving partial nitrification.Two groups of bacteria, namely ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) are involved in nitrification. Chemolitho-autotrophic AOB are responsible for the rate-limiting step of nitrification in a wide variety of environments, making them important in the global cycling of nitrogen. Characterization

show abstract

Population Dynamics and Nitrite Build-Up in Activated Sludge and Biofilm Processes for Nitrogen Removal

Cited by 15 publications

References 0 publications

Origin, causes and effects of increased nitrite concentrations in aquatic environments

Origin, causes and effects of increased nitrite concentrations in aquatic environments

Étude du processus de nitratation avec des boues activées : effet inhibiteur de l'ammoniac sur les bactéries nitratantes

Partial nitrification—operational parameters and microorganisms involved

Contact Info

Product

Resources

About