Population epigenetics, ecotoxicology and human diseases

Паткин, Е. Л.; Sofronov, Henry A

doi:10.17816/ecogen10414-28

Cited by 5 publications

(1 citation statement)

References 123 publications

(87 reference statements)

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“…Exposure to environmental toxicants can lead to heritable or reversible modifications that can affect gene expression without changing DNA structure, otherwise known as epigenetic changes [ 13 ]. The epigenetic processes, include DNA methylation, histone modifications, noncoding RNA regulation, chromatin structure remodeling, and RNA methylation.…”

Section: Introductionmentioning

confidence: 99%

Glyphosate and AMPA Induce Alterations in Expression of Genes Involved in Chromatin Architecture in Human Peripheral Blood Mononuclear Cells (In Vitro)

Woźniak

Reszka

Jabłońska

et al. 2021

IJMS

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We have determined the effect of glyphosate and aminomethylphosphonic acid (AMPA) on expression of genes involved in chromatin architecture in human peripheral blood mononuclear cells (PBMCs). The cells were incubated with glyphosate and AMPA in the concentrations ranging from 0.5 to 100 μM and from 0.5, to 250 μM, respectively. The expression profile of the following genes by quantitative Real-Time PCR was evaluated: Genes involved in the DNA methylation (DNMT1, DNMT3A) and DNA demethylation process (TET3) and those involved in chromatin remodeling: genes involved in the modification of histone methylation (EHMT1, EHMT2) and genes involved in the modification of histone deacetylation (HDAC3, HDAC5). Gene profiling showed that glyphosate changed the expression of DNMT1, DMNT3A, and HDAC3, while AMPA changed the expression of DNMT1 and HDAC3. The results also revealed that glyphosate at lower concentrations than AMPA upregulated the expression of the tested genes. Both compounds studied altered expression of genes, which are characteristic for the regulation of transcriptionally inactive chromatin. However, the unknown activity of many other proteins involved in chromatin structure regulation prevents to carry out an unambiguous evaluation of the effect of tested xenobiotics on the studied process. Undoubtedly, we have observed that glyphosate and AMPA affect epigenetic processes that regulate chromatin architecture.

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Section: Introductionmentioning

confidence: 99%

Glyphosate and AMPA Induce Alterations in Expression of Genes Involved in Chromatin Architecture in Human Peripheral Blood Mononuclear Cells (In Vitro)

Woźniak

Reszka

Jabłońska

et al. 2021

IJMS

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Toxicity of cadmium salts on indicators of embryogenesis of rats

Kolosova

Шаторна

2022

Regul. Mech. Biosyst.

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Cadmium is a toxic heavy metal which is considered a dangerous environmental pollutant and has a detrimental effect on the organs of the reproductive system, the period of implantation and the development of embryos. The experiment presented in this article established the effect of cadmium salts (chloride and citrate) on the general progress of embryogenesis. For this purpose, 60 rats were randomly divided into three groups: control, experimental group with cadmium chloride exposure and experimental group with cadmium citrate exposure. Cadmium chloride solvent, cadmium citrate solvent at a dose of 1.0 mg/kg and distilled intragastric water were injected from the first to the thirteenth (first subgroup) and from the first to the twentieth days of embryogenesis (second subgroup). When cadmium chloride was injected, total embryonic (by 4.24 and 3.67 times), pre-implantation (by 6.50 and 14.03 times) and post-implantation mortality (by 3.07 and 2.49 times) increased with a reduction of the number of surviving fetuses by 24.0% and 25.9% compared with the control group on the 13th and 20th days of embryogenesis respectively. At the same time, during exposure to cadmium citrate, indicators of total embryonic mortality increased by 4.02 and 3.52 times, pre-implantation mortality by 6.04 and 13.03 times, and post-implantation mortality by 3.09 and 2.26 times, and indicators of the number of live fetuses decreased by 18.3% and 22.2% in relation to the control group. When determining the accumulation of cadmium in embryos on the 20th day of gestation, polyelement analysis of biological materials using the atomic emission method with electric arc atomization revealed a 15.83-fold increase in cadmium chloride and 9.00 times in cadmium citrate relative to the control group. Embryolethality rates increased in animals of both experimental groups while the number of live fetuses per female decreased, which indicated an obvious embryotoxic effect of cadmium compounds. It is would be useful to conduct histological studies, which will help detect changes at the tissue level and possibly explain the level of embryonic mortality.

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DNA methylation in early mice embryogenesis under the influence of bisphenol A

Noniashvili

Михайловна²,

Грудинина

et al. 2017

Ecological genetics

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Background. Nonsteroid estrogen – bisphenol A (BPA) can have a detrimental effect on human health, and therefore poses a potential threat to humans. The critical window for the effect of BPA is the time of early development of the embryo, especially during the activation of the embryonic genome during development to the stage of blastocyst. Therefore, it is especially important to understand how DNA methylation is modified in embryos of the earliest developmental period under the influence of BPA. Materials and methods. Mice hybrids F1 (CBAXC57BL) were once administered 0, 8 mg of BPA per mouse and the level of DNA methylation was estimated by detection the fluorescence of antibodies against 5-MeC in nuclei of GD3 and GD9 embryos. In other series, the level of DNA methylation and the rate of blastocyst development were estimated following cultivation of one- and two cells embryos in the presence of BPA (50 or 100 µM) during 72-96 hours in vitro. Results. BPA exposure induced the decrease of the level of DNA methylation in GD3embryos received toxicant in utero, the amount of blastomeres in these embryos was decreased too. The level of DNA methylation in GD9 embryos was slightly higher than in control group. Upon cultivation of one-two cells embryos, BPA decreased the level of DNA methylation and the rate of embryos development to blastocyst stage. Conclusion. We have determined that early embryogenesis is highly sensitive period to the BPA effects. Such effect is most likely due to active reprogramming processes in this period, primarily related to DNA demethylation/methylation de novo of both the whole genome and individual genes.

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Population epigenetics, ecotoxicology and human diseases

Cited by 5 publications

References 123 publications

Glyphosate and AMPA Induce Alterations in Expression of Genes Involved in Chromatin Architecture in Human Peripheral Blood Mononuclear Cells (In Vitro)

Glyphosate and AMPA Induce Alterations in Expression of Genes Involved in Chromatin Architecture in Human Peripheral Blood Mononuclear Cells (In Vitro)

Toxicity of cadmium salts on indicators of embryogenesis of rats

DNA methylation in early mice embryogenesis under the influence of bisphenol A

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