Population estimate of the preantral follicles and frequency of multioocyte follicles in prepubertal and adult bitches

Lunardon, N.T.; Silva-Santos, Kátia Cristina; Justino, Rebeca Cordeiro; Dessunti, Gabriel T.; Seneda, Marcelo Marcondes; Martins, Maria Isabel Mello

doi:10.1016/j.theriogenology.2014.12.002

Cited by 14 publications

(8 citation statements)

References 17 publications

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“…As expected, primordial follicles were the most abundant, which was also consistent to previously reported in other species including cats 21 . The total number of follicles found in these three bitches was slightly higher than those already reported for adult bitches weighing less than 10 kg 20–22 . It has been shown in laboratory rodents that high concentrations of gonadotropins are necessary early in life to reach a full‐sized pool of follicles at the age of puberty 18 .…”

Section: Discussionsupporting

confidence: 90%

“…21 The total number of follicles found in these three bitches was slightly higher than those already reported for adult bitches weighing less than 10 kg. [20][21][22] It has been shown in laboratory rodents that high concentrations of gonadotropins are necessary early in life to reach a full-sized pool of follicles at the age of puberty. 18 Although the neonatal antagonist treatment did not seem to alter the total number of ovarian follicles, the number of primordial and antral follicles was increased in Bitch 1.…”

Section: Discussionmentioning

confidence: 99%

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Long‐term reproductive evaluation of a bitch neonatally treated with a gonadotropin‐releasing hormone agonist

Marchetti

Faya

Priotto

et al. 2021

Vet Record Case Reports

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In mammals, high concentrations of gonadotropins are necessary early in life to reach a full-sized pool of ovarian follicles at the age of puberty. This case report describes the reproductive status of a 28-month-old, 9.1 kg, crossbred bitch (Bitch 1) which had been treated neonatally with 18.8 deslorelin acetate subcutaneously for contraceptive purposes. The female had just passed pubertal oestrus when hormones were measured and then spayed. The ovaries were histologically assessed. Two crossbred bitches were used for comparisons. Bitch 1's anti-Müllerian hormone (AMH) and progesterone serum concentrations were 0.35 and 16.7 ng/ml, respectively. The total number of ovarian follicles and proportions were normal and the number of primary and antral follicles was increased. These findings might suggest an enhanced recruitment of the follicle pool to the growing population which could be related to the relatively low AMH concentrations.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Long‐term reproductive evaluation of a bitch neonatally treated with a gonadotropin‐releasing hormone agonist

Marchetti

Faya

Priotto

et al. 2021

Vet Record Case Reports

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“…13.6 follicles/mm 2 ) compared with dogs (4.5 follicles/mm 2 , or 7.8 follicles/mm 2 including the outlier). This inter-species difference has also been observed in previous work in our laboratory [67], although research in other labs have indicated that the overall preantral follicle population per ovary is higher in dogs [68,69] than cats [70], but these evaluations include additional follicle stages and consider the larger size of the typical dog ovary. As such, we postulate that the larger cat primordial follicle, and consequently oocyte, pool with substantive basal lamina may have also contributed to cat ovarian tensile strength in the absence of domestic dog levels of collagen HDM.…”

Section: Discussionsupporting

confidence: 81%

Comparative Tensile Properties and Collagen Patterns in Domestic Cat (Felis catus) and Dog (Canis lupus familiaris) Ovarian Cortical Tissues

Nagashima,

Zenilman,

Raab

et al. 2023

Bioengineering

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The importance of the ovarian extracellular environment and tissue rigidity on follicle survival and development has gained attention in recent years. Our laboratory has anecdotally observed differences in the rigidity of domestic cat and dog ovarian cortical tissues, which have been postulated to underlie the differences in in vitro culture responses between the species, wherein cat ovarian tissues display higher survival in extended incubation. Here, the tensile strengths of cat and dog ovarian cortical tissues were compared via micropipette aspiration. The underlying collagen patterns, including fiber length, thickness, alignment, curvature, branch points and end points, and overall tissue lacunary and high-density matrix (HDM) were quantified via picrosirius red staining and TWOMBLI analysis. Finally, we explored the potential of MMP (−1 and −9) and TIMP1 supplementation in modulating tissue rigidity, collagen structure, and follicle activation in vitro. No differences in stiffness were observed between cat or dog cortical tissues, or pre- versus post-pubertal status. Cat ovarian collagen was characterized by an increased number of branch points, thinner fibers, and lower HDM compared with dog ovarian collagen, and cat tissues exposed to MMP9 in vitro displayed a reduced Young’s modulus. Yet, MMP exposure had a minor impact on follicle development in vitro in either species. This study contributes to our growing understanding of the interactions among the physical properties of the ovarian microenvironment, collagen patterns, and follicle development in vitro.

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“…Most domestic species have been studied about the location and distribution of the ovarian follicular population. Some recent studies found peculiarities, such as the canine species, in which a different number of pre‐antral follicles was found between the right and left ovaries (Lunardon et al., ). In the bovine species, a distinction in the population of pre‐antral follicles in female Bos taurus taurus and Bos taurus indicus was observed (Silva‐Santos et al., ).…”

Section: Introductionmentioning

confidence: 99%

Regional distribution and integrity of equine ovarian pre‐antral follicles

Gonzalez¹,

Silva²,

Lindquist³

et al. 2017

Reprod Domestic Animals

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The goal of this study was to determine the distribution of pre-antral follicles in the ovarian parenchyma of mares. For Experiment 1, each ovary was cut longitudinally at the greater curvature, performing two hemiovaries. After that, six fragments from each hemiovary were obtained, resulting in 12 fragments, which were divided into the innermost region of the parenchyma, the middle region and the outermost region. All the three obtained sections were cut transversally to obtain two fragments from each one. For Experiment 2, each ovary also submitted to a longitudinal cut on the greater curvature, forming two hemiovaries. Each hemiovary was sectioned into four symmetrical fragments, resulting in eight fragments per ovary. The fragments were related as being near to or far from the ovulatory fossa. The fragments of both experiments were immediately fixed in Carnoy for 12 hr and kept in 70% ethanol for 24 hr. Follicles were classified according to the stages of development and for morphological integrity according to oocyte morphology and granulosa cells. After the histological assessment, a total of 1,130 follicles were visualized from Experiment 1, being 1,054 (93.3%) primordial follicles and 76 (4.7%) follicles in development. The innermost region had the highest percentage of pre-antral follicles compared to the other regions (p < .05). The middle and outermost regions showed higher percentages of intact primordial and developing follicles than the innermost region (p < .05). Considering Experiment 2, 938 follicles were found, being 894 (95.3%) primordial and 44 (4.7%) follicles in development. The region near the ovulatory fossa presented higher (58.7%; 551 of 938) follicular concentration compared to the region far from the ovulatory fossa (41.3%; 387 of 938; p < .05). As a conclusion, distribution of pre-antral follicles in the equine ovary has a specific pattern through the parenchyma. Also, the follicular integrity differed in the studied ovarian areas.

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Population estimate of the preantral follicles and frequency of multioocyte follicles in prepubertal and adult bitches

Cited by 14 publications

References 17 publications

Long‐term reproductive evaluation of a bitch neonatally treated with a gonadotropin‐releasing hormone agonist

Long‐term reproductive evaluation of a bitch neonatally treated with a gonadotropin‐releasing hormone agonist

Comparative Tensile Properties and Collagen Patterns in Domestic Cat (Felis catus) and Dog (Canis lupus familiaris) Ovarian Cortical Tissues

Regional distribution and integrity of equine ovarian pre‐antral follicles

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