Population genetic analysis and conservation strategies for redtail shrimp Fenneropenaeus penicillatus using ten microsatellite markers

Abstract

ABSTRACT.Fenneropenaeus penicillatus, which is on the Red List of Endangered Species for China, is an important shrimp species. However, there is not enough genetic information on F. penicillatus for conservation and management purposes. Ten microsatellite markers were used to analyze the genetic diversity, genetic differentiation, and population structure of F. penicillatus to provide scientific information for the conservation of the species. Low genetic diversity and moderate genetic differentiation were found among 12 putative populations [Beihai, Dongshan (DS), Hainan (HN), Lianjiang, Nanao (NA), Ningde (ND), Putian, Quanzhou (QZ), Xiamen (XM), Shenzhen, Zhanjiang, and Zhangpu] along the southeast coast of China. QZ, XM, and DS exhibited the highest genetic diversity, while NA and ND had the lowest genetic diversity. Genetic differentiation among all populations, except HN, was low compared to the genetic differentiation between HN and the other 11 putative populations. These 12 putative populations were divided into two subgroups. One group consisted of XM, DS, and QZ. The other group consisted of the other eight putative populations with the exception of HN. The HN Island population requires further study due to its large genetic distance from the other 11 putative populations. Problems with the current conservation strategy are pointed out and suggestions given based on genetic information.

Inferring the impact of large-scale aquaculture of Haliotis discus hannai in northern and southern China using EST-SSR markers

“…The 20 population loci deviated signi cantly from HWE (P < 0.004), and the inbreeding coe cient Fis was positive (range 0.0215-0.6073). It is inferred that the reason for the signi cant deviation from HWE is heterozygous deletion (Cao et al, 2017). However, none of the 13 loci in the wild population were observed to deviate from HWE, indicating that the breeding population may be prone to nonrandom mating, arti cial or natural selection during the breeding process, and the occurrence of inbreeding, which may lead to loss of heterozygotes.…”

“…Microsatellites or simple sequence repeats (SSRs), which are tandem repeats of short (1-6 bp long) sequences (Holland et al, 2001;Zane et al, 2002), have many ideal traits, such as high polymorphism, high variability, codominance, speci city under polymerase chain reaction (PCR) ampli cation, and easy analysis of results. The markers have been widely used in population genetics research on sh (Yi et al, 2015), shrimp (Cao et al, 2017), shell sh (Reece et al,2004;Nie et al, 2017), and others. SSRs include genomic SSRs and expressed sequence tag SSRs (EST-SSRs).…”

Conservation Genetics of the Asian Giant Soft-Shelled Turtle (Pelochelys cantorii) with Novel Microsatellite Multiplexes

“…Microsatellite markers are codominant markers with a high degree of polymorphism [ 3 , 4 , 5 ]. They are effective molecular markers in the field of population genetic diversity detection as well as genetic map construction [ 6 , 7 , 8 ]. With the rapid development of high-throughput sequencing technology, it is now easier and cheaper to screen microsatellite markers, which also promotes their wide application.…”