Population genetic analysis and sub-structuring of Theileria parva in Uganda

Oura, C.A.L.; Asiimwe, Benon B.; Weir, William; Lubega, George W.; Tait, Andy

doi:10.1016/j.molbiopara.2004.12.015

Cited by 75 publications

(91 citation statements)

References 27 publications

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“…This would suggest that small population sizes or bottleneck events are the norm in Theileria biology (McKeever, 2009). In this regard, T. parva was assumed to be panmictic, but has been shown to exhibit local sub-structuring in different populations (Oura et al 2005;Muleya et al 2012). The effective population size of a Theileria parasite in a buffalo with moderate parasitaemia (0·01%) is, however, not small but in the range of 1 × 10 10 parasites.…”

Section: Discussionmentioning

confidence: 99%

Geographic distribution of Theileria sp. (buffalo) and Theileria sp. (bougasvlei) in Cape buffalo (Syncerus caffer) in southern Africa: implications for speciation

et al. 2013

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Strict control measures apply to movement of buffalo in South Africa including testing for Theileria parva, the causative agent of Corridor disease in cattle. The official test is a real-time hybridization PCR assay that amplifies the 18S rRNA V4 hyper-variable region of T. parva, T. sp. (buffalo) and T. sp. (bougasvlei). Mixed infections with the latter organisms affect diagnostic sensitivity due to PCR suppression. While the incidence of mixed infections in the Corridor disease endemic region of South Africa is significant, little information is available on the specific distribution and prevalence of T. sp. (buffalo) and T. sp. (bougasvlei). Specific real-time PCR assays were developed and a total of 1211 samples known to harbour these parasites were screened. Both parasites are widely distributed in southern Africa and the incidence of mixed infections with T. parva within the endemic region is similar (* 25-50%). However, a significant discrepancy exists in regard to mixed infections of T. sp. (buffalo) and T. sp. (bougasvlei) (* 10%). Evidence for speciation between T. sp. (buffalo) and T. sp. (bougasvlei) is supported by phylogenetic analysis of the COI gene, and their designation as different species. This suggests mutual exclusion of parasites and the possibility of hybrid sterility in cases of mixed infections.

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Section: Discussionmentioning

confidence: 99%

Geographic distribution of Theileria sp. (buffalo) and Theileria sp. (bougasvlei) in Cape buffalo (Syncerus caffer) in southern Africa: implications for speciation

et al. 2013

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“…DNA was purified from bovine blood samples spotted on to FTA filter paper (Whatman BioScience) according to a previously described protocol (Oura et al 2005).…”

Section: Sample Preparationmentioning

confidence: 99%

“…The inner and outer nested primers were designed from sequences in the flanking regions of two micro-and ten mini-satellite repeats (ms5,ms7,MS 3,MS 7,MS 8,MS 16,MS 19,MS 21,MS 25,MS 27,MS 33 and MS 40). The copy number and consensus repeat sequences as well as the sequences of the inner nested primers have been described (Oura et al 2003) as well as the sequences of the outer nested primers (Oura et al 2004a(Oura et al , 2005. The conditions used in the nested PCR amplifications are as described previously (Oura et al 2004a).…”

Section: Pcr Amplificationmentioning

confidence: 99%

Theileria parva genetic diversity and haemoparasite prevalence in cattle and wildlife in and around Lake Mburo National Park in Uganda

et al. 2010

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Wildlife, especially Cape buffalo (Syncerus caffer), are thought to act as a reservoir for many of the important tick-borne pathogens of cattle. In this study, we have determined the prevalence of the most significant tick-borne haemoparasites in wildlife (buffalo, impala, eland and bushbuck) as well as in cattle grazing inside and neighbouring Lake Mburo National Park (LMNP) in Uganda. A high percentage of buffalo were carriers of Theileria parva, Theileria mutans, Theileria velifera, Theileria buffeli and Theileria sp. (buffalo) as well as Anaplasma marginale and Anaplasma centrale. The majority of impala sampled were carriers of A. centrale, and all were carriers of an unidentified Babesia/Theileria species. The eland and bushbuck sampled were all carriers of Theileria taurotragi and Theileria buffeli, and the majority were carriers of T. mutans. The bushbuck sampled were also carriers for Erhlichia bovis. There were some differences in the prevalence of haemoparasites between the calves sampled inside and neighbouring LMNP. In order to address the question of whether there is evidence for interbreeding between buffalo-associated and cattle-associated T. parva populations, multi-locus genotypes (MLGs) of T. parva (based on micro-satellite markers) from buffalo and from calves grazing inside and outside LMNP were compared, and the results revealed that buffalo and cattle gene pools were distinct, showing no evidence for transmission of buffalo-derived T. parva genotypes to the cattle population.

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“…Population genetic studies of T. annulata have previously been performed using a panel of micro-and mini-satellite markers (Weir et al, 2007). This genotyping approach has also been applied to other Apicomplexan species such as Plasmodium falciparum (Anderson et al, 2000) and Theileria parva, a phylogenetically close relative of T. annulata (Oura et al, 2005;Muleya et al, 2012). A study within Tunisian and Turkish T. annulata populations demonstrated that genetic differentiation could be detected between geographically separated populations (Weir et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Population diversity of Theileria annulata in Portugal

Gomes

Salgueiro

Inácio

et al. 2016

Infection, Genetics and Evolution

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The tick-borne protozoan parasite Theileria annulata causes tropical theileriosis, a severe disease of cattle that occurs across the Mediterranean littoral, the Middle East and Southern Asia. In the Mediterranean region, the disease has long been perceived as being a constraint to livestock production in North Africa and Turkey but was believed to have minimal impact in Southern European countries. It has recently been demonstrated that in Southern Portugal the prevalence of T. annulata is approximately 30%. While the population genetics of the parasite and the multiplicity of infection in the bovine host have been studied in a number of countries, no information is currently available on the composition of the parasite population in Southern Europe or its relationship to populations in bordering regions. A parasite genotyping system, based on micro-and mini-satellite amplification, was used to perform genetic analysis of T. annulata populations from T. annulata infected cattle in twelve farms in Southern Portugal. A diversity of genotypes and a high multiplicity of infection were found, suggesting that the parasite possesses a panmictic population in this region. In comparison with genotypes found in Tunisia and Turkey, parasites from Portugal form a genetically distinct group and show lower genetic diversity.

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Population genetic analysis and sub-structuring of Theileria parva in Uganda

Cited by 75 publications

References 27 publications

Geographic distribution of Theileria sp. (buffalo) and Theileria sp. (bougasvlei) in Cape buffalo (Syncerus caffer) in southern Africa: implications for speciation

Geographic distribution of Theileria sp. (buffalo) and Theileria sp. (bougasvlei) in Cape buffalo (Syncerus caffer) in southern Africa: implications for speciation

Theileria parva genetic diversity and haemoparasite prevalence in cattle and wildlife in and around Lake Mburo National Park in Uganda

Population diversity of Theileria annulata in Portugal

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