Population Genetic Data Determined For Five Different Single Locus Minisatellite Probes

Recent observations in human DNA-minisatellite mutations

“…This has to be taken into consideration, especially in parentage testing. In previous papers we reported mutation rates at VNTR loci [10,11]. Due to the increased number of observations, we are now able to update and to review earlier data and to describe the expansion of repeats in mutant paternal alleles at 2 loci.…”

“…It is well known [10,11,23,27] that probes MS31, G3 and MS43A detect low-mutant loci, while the MS 1 mutation rate is substantially higher. The observed minisatellite mutation frequencies are in accordance with published findings [23,27].…”

“…Techniques for DNA isolation, endonuclease restriction, electrophoresis and hybridizations have been described elsewhere [ 10]. Membranes were hybridized consecutively with probes MS 1, MS31, MS43A, G3, YNH24 and MS205 [22,26,32].…”

Evaluation of the genetic affinity between populations based on the comparison of allele distributions in two highly variable DNA regions

“…The following graphs contain the comparison of the Polish population with other populations of Europe: Austrian [4], German [5] and English [3] as well as the comparison of the Polish population with the Asian and Afro-Caribbean populations [6] were presented in Fig. 1 and Fig.…”

“…In the HVR locus we observe a high number of variants in the amount of tandem repetitions and a low frequency within population [3]. The graphic analysis based on every 100 base pairs alleles frequency intervals proved the similar distribution of alleles in both the Polish and other Caucasian populations of Europe with characteristic distributions-a mono-modal in locus D7S21 and bi-modal in locus D12S11 [4][5][6]. Significant differences in the structure of distributions when we compared the investigated Polish population representing Caucasians with Asian population and especially Afro-Caribbean population support the previous observations that the distribution of HVR alleles between populations isolated territorially or represented different human race are quite distinct from each other [7].…”