Population genetic structure in a self‐compatible hermaphroditic snail is driven by drift independently of its contemporary mating system

Abstract

Genetic drift, gene flow, and natural selection commonly influence population genetic diversity. In populations of self‐compatible hermaphrodites, the mating system (e.g., self‐fertilization) further reduces individual heterozygosity. Furthermore, selfing, as a form of inbreeding, significantly impacts genetic drift by reducing effective population size (Ne). This can potentially accelerate genetic drift, particularly in small populations where self‐fertilization is likely during founder events. To investigate the roles of genetic drift and contemporary mating system in populations of the freshwater snail Lymnaea stagnalis, we examined their effective population sizes (Ne) and Tajima's D values, which reflect genetic drift over extended time periods, as well as estimates of within‐population selfing rates and pairwise relatedness reflecting contemporary mating system. We used 4054 SNP markers obtained using restriction site associated DNA (RAD) sequencing from individuals in five snail populations originating from geographically closely located ponds. We found strong population genetic structure and differences in genetic diversity among populations. Covariation between genetic diversity and Ne estimates and Tajima's D values suggested drift being an important determinant of genetic diversity and structure in these populations. However, this effect was independent of the contemporary mating system, as indicated by the similarity of selfing rates and relatedness estimates among populations. Thus, founder events (possibly including historical inbreeding) and/or drift due to small sizes of L. stagnalis populations are likely to explain their genetic structure and limit within‐population genetic diversity.