Population genetic study in Hungarian canine populations using forensically informative STR loci

Zenke, Petra; Egyed, Balázs; Zöldág, L.; Pádár, Zsolt

doi:10.1016/j.fsigen.2010.03.013

Cited by 26 publications

(16 citation statements)

References 13 publications

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“…Moreover, the inbreeding coefficient (F IS ) was 0.053 for the whole analyzed beagle population which does not exceed the critical value of 12.5% (54), thus represents no significant level of inbreeding. Comparing the obtained F IS to other breeds in Hungary, analyzed by a partially overlapping marker set, our results were similar to those of Dachshunds but somewhat smaller than in German shepherds or Giant Schnauzers (36). As to date there is no published STR data available from Hungarian beagles, or from worldwide populations, we compared our F IS data to a Polish beagle dataset that was collected from the Cracow area, and inbreeding was calculated based on pedigree documents (79).…”

Section: Discussionsupporting

confidence: 57%

“…To test this we analyzed the genetic diversity and the genetic structure in the examined beagle stocks. A subset of the investigated 11 microsatellite markers have been previously examined in a canine population genetic study (36), that aimed to develop so-called "mini PCR primer sets" for genetic testing of canine plucked hairs or degraded remains (45). During this research each marker has been proved to be an effective tool for investigating population substructure in dogs.…”

Section: Discussionmentioning

confidence: 99%

“…In the human genome, thousands of such microsatellite markers have been identified (32), accounting for nearly 3% of our DNA (33) and the same ratio is expected in the dog genome (34). Microsatellites are commonly used in population genetic studies in canine species (35,36), in forensic caseworks (37,38) and in conservation biology (39,40) due to their Mendelian inheritance and informativeness. They are neutral, multi-allelic and have greater discriminatory power than biallelic markers such as SNPs.…”

Section: Genotyping Of Population Samplesmentioning

confidence: 99%

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Comparison of Behavior and Genetic Structure in Populations of Family and Kenneled Beagles

et al. 2020

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In dogs, the social and spatial restriction associated with living in a kennel environment could lead to chronic stress and the development of abnormal behaviors ("kennel-dog syndrome"). However, little is known about how kenneled dogs differ from their conspecifics living as pets in human families. In the current study, using a test battery exposing the dogs to novel stimuli, we compared the behavior of three groups of beagles: (1) kenneled dogs living in a restricted environment with limited human contact (N = 78), (2) family dogs living in human families as pets (N = 37), and (3) adopted dogs born in the kenneled population but raised in human families (N = 13). We found one factor comprising most of the test behaviors, labeled as Responsiveness. Family dogs and adopted dogs scored higher in Responsiveness than kenneled dogs. However, 23% of the kenneled dogs were comparable to family and adopted dogs based on a cluster analysis, indicating a similar (positive) reaction to novel stimuli, while 77% of the kenneled dogs were unresponsive (mostly immobile) in at least part of the test. To assess if the behavioral difference between the family and kenneled dogs could be due to genetic divergence of these two populations and/or to lower genetic diversity of the kenneled dogs, we analyzed their genetic structure using 11 microsatellite markers. We found no significant difference between the populations in their genetic diversity (i.e., heterozygosity, level of inbreeding), nor any evidence that the family and kenneled populations originated from different genetic pools. Thus, the behavior difference between the groups more likely reflects a G × E interaction, that is, the influence of specific genetic variants manifesting under specific environmental conditions (kennel life). Nevertheless, some kenneled individuals were (genetically) more resistant to social and environmental deprivation. Selecting for such animals could strongly improve the welfare of kenneled dog populations. Moreover, exploring the genetic background of their higher resilience could also help to better understand the genetics behind stress-and fear-related behaviors.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Genotyping Of Population Samplesmentioning

confidence: 99%

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Comparison of Behavior and Genetic Structure in Populations of Family and Kenneled Beagles

et al. 2020

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“…The development of short tandem repeat (STR) analysis has streamlined human individualization and now allowed for DNA genotyping of many additional species such as cat (17,18), dog (19)(20)(21)(22)(23)(24), horse (25)(26)(27)(28)(29)(30), and marijuana (31)(32)(33)(34)(35)(36)(37) that have significant value as trace evidence. There are several scientific reports about STR markers in development for Cannabis sativa (31)(32)(33)(34)(35)(36)(37).…”

mentioning

confidence: 99%

Field Testing of Collection Cards for Cannabis sativa Samples With a Single Hexanucleotide DNA Marker*,†

Allgeier

Hemenway

Shirley

et al. 2011

Journal of Forensic Sciences

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The validity and feasibility of using DNA collection cards in the field for preservation and analysis of Cannabis sativa genotypes were investigated using a highly specific hexanucleotide marker. Collection cards were submitted to the National Marijuana Initiative, which selectively trained and managed the collection of specific types of samples from a variety of participating agencies. Samples collected at seizure sites included fresh marijuana leaf samples, dried "dispensary" samples, U.S. border seizures, and hashish. Using a standardized PCR kit with custom-labeled oligonucleotide primers specific to marijuana, collection cards produced eight genotypes and 13 different alleles, extremely low baselines, and no cross-reactivity with control plant species. Results were produced from all sample types with the exception of hashish. Plant DNA collection cards represent an easily implementable method for the genetic identification and relatedness of C. sativa street and grow site-seized samples with applications for databasing and market disruption.

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“…The quality requirement for implementation of canine DNA analysis in forensic practice is as ambitious as in human forensics. The STR marker-sets which have been developed have relevant population studies, occasionally extended with genetic variance [258][259][260][261][262][263][264], inbreeding [258,261] and mutation data [265]. Sequence databases of canine mitochondrial DNA have also been developed [266][267][268].…”

Section: Dna Analysis Of Nonhuman Remainsmentioning

confidence: 99%

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

Pádár¹,

Zenke²,

Kozma³

2018

Post Mortem Examination and Autopsy - Current Issues From Death to Laboratory Analysis

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The importance of biological traces and evidences related to a criminal matter has been recognized for a long time. The examination of the expression of genetic polymorphism has been an integral part of the multidisciplinary field of medico-legal autopsy for over a century. Since the initial application of blood group antigens for personalization of a putative perpetrator in a murder case, the discipline of forensic genetics has evolved as a standard of forensic sciences. The real breakthrough, the application of molecular tools and processes for the in-vitro replication of genetic substances, has increasingly allowed the exploitation of advances of molecular genetics for both forensic and criminal investigations. Although there are certainly many more applications and scientific fields in the medico-legal arena, the relatively fast progress of genetics, which has accelerated recently with state-of-art technologies, can provide ever more relevant information in relation to a corpse or the cause and manner that resulted in the corpse for autopsy. This topic concerns the currently accepted forensic DNA technology, and the last section reviews commonly used markers for nuclear and mitochondrial DNA analysis as well as ongoing research. This review also focuses on the increasingly important non-human sources of DNA, and shortly covers the main aspects of animal forensic DNA examination.

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Population genetic study in Hungarian canine populations using forensically informative STR loci

Cited by 26 publications

References 13 publications

Comparison of Behavior and Genetic Structure in Populations of Family and Kenneled Beagles

Comparison of Behavior and Genetic Structure in Populations of Family and Kenneled Beagles

Field Testing of Collection Cards for Cannabis sativa Samples With a Single Hexanucleotide DNA Marker*,†

Forensic DNA Technological Advancements as an Emerging Perspective on Medico-Legal Autopsy: A Mini Review

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