Population genomics of Staphylococcus pseudintermedius in companion animals in the United States

Smith, Joshua T.; Amador, Sharlene; McGonagle, Colin J.; Needle, David B.; Gibson, R. J.; Andam, Cheryl P.

doi:10.1038/s42003-020-1009-y

Cited by 34 publications

(32 citation statements)

References 69 publications

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“…Almost 85% and 23% of isolates in the current study harbored bla Z and mec A genes, respectively. These findings supported those of Smith et al 34 . The previous study also showed a high percentage (over 80%) of multidrug-resistant isolates among MRSPs 7 .…”

Section: Discussionsupporting

confidence: 92%

“…This result corresponded to the frequent presence of genetic determinants of tetracycline resistance in isolates from healthy cats. The widespread presence of tet (K) and tet (M) in S. pseudintermedius isolates was previously described 34 . S. pseudintermedius isolates from sick animals were more frequently phenotypically and genetically resistant to beta-lactams, limiting the successful application of antibiotics in treatments.…”

Section: Discussionmentioning

confidence: 91%

“…The prevalence of S. pseudintermedius in cats was previously reported as 2.49–8.8%, while that of dogs exceeded 90% 2 – 6 , 33 . Thus, S. pseudintermedius might not be a natural microbiota of cats 34 . The previously reported prevalence of S. aureus was higher in healthy cats (13.62%) 15 compared to that obtained for S. pseudintermedius in the current study (2.49%).…”

Section: Discussionmentioning

confidence: 99%

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Epidemiology of Staphylococcus pseudintermedius in cats in Poland

Bierowiec

Miszczak

Korzeniowska‐Kowal

et al. 2021

Sci Rep

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Staphylococcus pseudintermedius is a well-known coagulase-positive staphylococcus that is mainly associated with the asymptomatic colonization of the skin of pets and mucous membranes. Little is still known about the occurrence of S. pseudintermedius in cats. The current study aimed to characterize the isolates of S. pseudintermedius from sick and healthy cats. This was achieved by examining their antibiotic resistance properties, biofilm formation, and genotype differences. Six hundred and seventy-six cats were swabbed (595 healthy and 81 sick cats). Thirty-five distinct S. pseudintermedius isolates from 27 cats were isolated. The prevalence of S. pseudintermedius in healthy and sick cats was 2.49% and 7.61%, respectively. In comparison, MRSP (methicillin-resistant Staphylococcus pseudintermedius) prevalence was 0.12% and 2.98%, respectively. Cats were more frequently colonized with S. pseudintermedius when kept with dogs, regardless of their health condition, with this result being statistically significant. Multidrug resistance was detected in 50%, and 38.46% of S. pseudintermedius isolates from healthy and sick cats, respectively. In contrast, genetic multidrug resistance was detected in 59% and 46.15% cases, respectively. Seven from eight isolated MRSPs were multidrug-resistant. Multi-locus sequence typing (MLST) assigned isolates to 19 types, of which 16 types submitted for the first time to the PubMLST database. The most frequently detected STs (sequence types) were 551 and 71. ST71 and ST551 were mainly isolated from cats with clinical signs of infection. All were MRSPs, regardless of cats’ health. These isolates were characterized with the most frequent antibiotic resistance at the phenotypic and genotypic level.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Epidemiology of Staphylococcus pseudintermedius in cats in Poland

Bierowiec

Miszczak

Korzeniowska‐Kowal

et al. 2021

Sci Rep

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“…Both MRSA and MRSP display resistance to β-lactam and many non-β-lactam antimicrobial classes, such as fluoroquinolones, aminoglycosides, tetracyclines, sulfonamides, macrolides, and lincosamides (18)(19)(20)(21)(22). However, MRSP isolates from companion animals have been reported to be more multidrug-resistant than MRSA (23). A recent study has revealed that many clinical MRSP multidrug-resistant clones have emerged through multiple independent, horizontal acquisition of resistance determinants and frequent genetic exchange that disseminate DNA to the broader population (24).…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology of Clinical and Colonizing Methicillin-Resistant Staphylococcus Isolates in Companion Animals

et al. 2021

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In this study, we aimed to investigate the molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) clinical and colonizing isolates of dogs and cats to profile contributing factors associated with their isolation. Nasal and rectal samples were collected from dogs and cats between 2015 and 2017 to identify colonizing isolates. Clinical isolates collected between 2003 and 2016 were retrieved from a Queensland university veterinary diagnostic laboratory. All isolates were identified using standard microbiological and molecular methods and were characterized by whole genome sequencing. Phylogenetic relationships and differences in epidemiological factors were investigated. Seventy-two MRSP isolates out of 1,460 colonizing samples and nine MRSP clinical isolates were identified. No MRSA was isolated. ST496 and ST749 were the most commonly isolated sequence types with different SCCmec types. ST496 clones spread both along the coast and more inland where ST749 was more centered in Brisbane. The resistance and virulence factors differed significantly between the two sequence types. ST496 colonizing and clinical isolates were similarly multidrug resistant. The virulence genes of ST749 colonizing and clinical isolates were similar as both contained the gene nanB for sialidase. There were no differences in the individual and clinical factors between predominant sequence types. High levels of antimicrobial resistance occurred in the majority of isolates, which is of potential concern to human and veterinary health. The phylogenetic clustering of isolates from this study and others previously identified in countries, particularly New Zealand, with which Australia has high volume of pet movements could suggest the importation of clones, which needs further investigation.

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“…The global emergence and rapid dissemination of MRSP is a serious health threat in veterinary and human medicine [ 2 , 12 , 13 ]. Remarkably, MRSP and other multidrug-resistant clones of various sequencing types are spreading globally with steadily increasing incidence and prevalence rates in both healthcare and community settings [ 2 , 13 , 45 , 46 ]. Although large-scale genome sequencing projects and several epidemiological studies have gained new insights into the complexity of MRSP evolution and diversity, general understanding of S. pseudintermedius virulence and immune evasive attributes is still very limited.…”

Section: Discussionmentioning

confidence: 99%

Methicillin-resistant Staphylococcus pseudintermedius synthesizes deoxyadenosine to cause persistent infection

et al. 2021

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Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is an emerging zoonotic pathogen of canine origin that causes an array of fatal diseases, including bacteremia and endocarditis. Despite large-scale genome sequencing projects have gained substantial insights into the genomic landscape of MRSP, current knowledge on virulence determinants that contribute to S. pseudintermedius pathogenesis during human or canine infection is very limited. Using a panel of genetically engineered MRSP variants and a mouse abscess model, we here identified the major secreted nuclease of S. pseudintermedius designated NucB and adenosine synthase A (AdsA) as two synergistically acting enzymes required for MRSP pathogenesis. Similar to Staphylococcus aureus, S. pseudintermedius requires nuclease secretion along with the activity of AdsA to degrade mammalian DNA for subsequent biosynthesis of cytotoxic deoxyadenosine. In this manner, S. pseudintermedius selectively kills macrophages during abscess formation thereby antagonizing crucial host immune cell responses. Ultimately, bioinformatics analyses revealed that NucB and AdsA are widespread in the global S. pseudintermedius population. Together, these data suggest that S. pseudintermedius deploys the canonical Nuc/AdsA pathway to persist during invasive disease and may aid in the development of new therapeutic strategies to combat infections caused by MRSP.

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Population genomics of Staphylococcus pseudintermedius in companion animals in the United States

Cited by 34 publications

References 69 publications

Epidemiology of Staphylococcus pseudintermedius in cats in Poland

Epidemiology of Staphylococcus pseudintermedius in cats in Poland

Molecular Epidemiology of Clinical and Colonizing Methicillin-Resistant Staphylococcus Isolates in Companion Animals

Methicillin-resistant Staphylococcus pseudintermedius synthesizes deoxyadenosine to cause persistent infection

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