Population of Aphanizomenon from the Gulf of Gdańsk (Southern Baltic Sea): differences in phenotypic and genotypic characteristics

Palińska, Katarzyna A.; Surosz, Waldemar

doi:10.1007/s10750-008-9388-y

Cited by 10 publications

(5 citation statements)

References 26 publications

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“…Traditional microscopy may also overestimate the richness of phytoplankton. Several examples from the literature demonstrate that different phenotypes and transition types of a given phytoplankton species may be identified as separate species [29]. The change in phenotype due to variations in environmental conditions may also cause conspecific individuals to be identified as distinct species [30].…”

Section: Discussionmentioning

confidence: 99%

Use of High Throughput Sequencing and Light Microscopy Show Contrasting Results in a Study of Phytoplankton Occurrence in a Freshwater Environment

et al. 2014

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Assessing phytoplankton diversity is of primary importance for both basic and applied ecological studies. Following the advances in molecular methods, phytoplankton studies are switching from using classical microscopy to high throughput sequencing approaches. However, methodological comparisons of these approaches have rarely been reported. In this study, we compared the two methods, using a unique dataset of multiple water samples taken from a natural freshwater environment. Environmental DNA was extracted from 300 water samples collected weekly during 20 years, followed by high throughput sequencing of amplicons from the 16S and 18S rRNA hypervariable regions. For each water sample, phytoplankton diversity was also estimated using light microscopy. Our study indicates that species compositions detected by light microscopy and 454 high throughput sequencing do not always match. High throughput sequencing detected more rare species and picoplankton than light microscopy, and thus gave a better assessment of phytoplankton diversity. However, when compared to light microscopy, high throughput sequencing of 16S and 18S rRNA amplicons did not adequately identify phytoplankton at the species level. In summary, our study recommends a combined strategy using both morphological and molecular techniques.

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Section: Discussionmentioning

confidence: 99%

Use of High Throughput Sequencing and Light Microscopy Show Contrasting Results in a Study of Phytoplankton Occurrence in a Freshwater Environment

et al. 2014

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“…Nevertheless, molecular data retrieved from the 16 S rDNA sequence, SSU rDNA and PC-IGS analysis place this particular population of Aphanizomenon close to A. flosaquae, suggesting that only one species occurs in brackish waters (Laamanen et al, 2002;Stal et al, 2003). Features shared by Anabaena, Aphanizomenon and Nodularia genera are their diazotrophic metabolism (capability to fix N 2 ) and the presence of gas vesicles, which favours their occurrence and proliferation in eutrophic waters (Laamanen et al, 2002;Stal et al, 2003;Palinska & Surosz, 2008). Ã Salinity was measured using the Practical Salinity Scale.…”

Section: Introductionmentioning

confidence: 93%

“…Some of these distinct environments are highly contaminated, like other brackish-water systems around the world (Telesh, 2004;Segal et al, 2006;Paoli et al, 2007). The phytoplankton of the Baltic is dominated during summer by filamentous, bloom-forming cyanobacteria, in particular Nodularia, Anabaena and Aphanizomenon (Wille´n & Mattsson, 1997;Laamanen et al, 2002;Karjalainen et al, 2007;Palinska & Surosz, 2008). Two distinct genotypes of Nodularia have been detected in the Baltic Sea through 16 S rRNA gene sequence analysis and total genome techniques.…”

Section: Introductionmentioning

confidence: 99%

Planktonic and benthic cyanobacteria of European brackish waters: a perspective on estuaries and brackish seas

Lopes

Vasconcelos

2011

European Journal of Phycology

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Cyanobacteria are photosynthetic organisms found in aquatic and terrestrial biotopes and ecosystems throughout the world. Marine and freshwater cyanobacteria have been extensively studied due to the toxic hazards that some of them create and biotechnological interest. In contrast, the cyanobacteria of brackish waters have been less studied despite being able to form toxic blooms like their freshwater and marine counterparts. We review the occurrence, diversity and toxicity of cyanobacteria species in the brackish waters of Europe, mainly estuaries and brackish environments of the Atlantic Ocean and the Mediterranean and Baltic Seas. The dominant cyanobacteria belong mainly to the planktonic genera Nodularia, Aphanizomenon, Microcystis and Anabaena, but also the benthic forms of Anabaena and Phormidium. Most genera from brackish waters are reported to be hepatotoxin producers (microcystin and nodularin). However, anatoxin-a and other bioactive compounds (e.g. apoptogens) can also be found and are produced exclusively by benthic forms. Nodularin is the best-characterized brackish-water cyanotoxin, being primarily produced by N. spumigena. Data are presented on cyanotoxin production, accumulation, and potential food chain transfer, with a particular focus on nodularin, which induces multiple effects on food-chain dynamics. Microcystins and anatoxin-a are also considered. Potential risks to animals and humans from cyanotoxin exposure are discussed, although reports of animal poisoning by brackish-water toxic cyanobacteria are scarce and no cases of human intoxication are yet known.

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“…Genotype differences between species tend to be small; and it is sometimes difficult to detect them during such studies. Phenotype differences arise primarily from the reaction of an organism to changing environments (Palińska, Surosz 2008). Therefore, for the purposes of full taxonomic verification of the genus Anabaena studied, it is advisable to apply the polyphasic approach, with which it is possible to draw the most precise conclusions concerning the phylogenesis of the studied cyanobacteria genus.…”

Section: Discussionmentioning

confidence: 99%

“…The pairs of primers used to study the 16S rRNA fragment were CYA106F/CYA781R and CYA359F/CYA781R (Nübel et al 1997), while the analysis of 16S-23S rRNA (ITS) was based on primer pairs 16CITS/23CITS (Neilan 2002, Laamanen et al 2002 and another pair specific to the genus Anabaena -Ana2 (F)/Ana4 (R) (Fergusson, Saint 2000). PCR conditions were as described in Palińska and Surosz (2008). DNA separation with DGGE was performed using an INGENYphorU-2 apparatus.…”

Section: Dna Isolation Pcr Dggementioning

confidence: 99%

Polyphasic characterization of the genus Anabaena from the Gulf of Gdańsk (Southern Baltic Sea). Application of DGGE in taxonomic studies on environmental samples

Siedlewicz

Forycka

Surosz

2012

Oceanological and Hydrobiological Studies

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The aim of this paper was a taxonomic verification of cyanobacteria of the genus Anabaena that occur in the Gulf of Gdańsk. Classical taxonomic methods were combined with modern molecular taxonomic methods (polyphasic approach). Analysis of the species diversity of cyanobacteria from the genus Anabaena was based on the microscopic analysis and on the analysis of 16S rRNA and ITS region. Comparison of the obtained results with sequences in GenBank showed 97.8-100% similarity for 16S rRNA and 98.8-100% similarity in the case of the ITS fragment. Similarity of the 16S rRNA and ITS sequence of 98.5% seems to be sufficient to determine the species.

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Population of Aphanizomenon from the Gulf of Gdańsk (Southern Baltic Sea): differences in phenotypic and genotypic characteristics

Cited by 10 publications

References 26 publications

Use of High Throughput Sequencing and Light Microscopy Show Contrasting Results in a Study of Phytoplankton Occurrence in a Freshwater Environment

Use of High Throughput Sequencing and Light Microscopy Show Contrasting Results in a Study of Phytoplankton Occurrence in a Freshwater Environment

Planktonic and benthic cyanobacteria of European brackish waters: a perspective on estuaries and brackish seas

Polyphasic characterization of the genus Anabaena from the Gulf of Gdańsk (Southern Baltic Sea). Application of DGGE in taxonomic studies on environmental samples

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