The research work was carried out in different agro-eco-zones of Sindh, Pakistan at the experimental field area of Cotton Agriculture Research Station at Tandojam, Sakrand, Kotdiji and Sarhad, 2019. The peak population of the Dusky cotton bug (DCB) was recorded at Tandojam. The high population of natural enemies like Chrysoperla, Orius, and Mirid bug was observed from July to September. The peak pest of pest observed averagely at Tandojam and Sakrand (49 to 23%). The abiotic factors like temperature and humidity averagely at Tandojam and Sakrand (32 to 50%) followed by Kotdiji and Sarhad (41 to 18%) recorded ideal for Chrysoperla, Orius, and Mirid bug. The abiotic factors were recorded as favorable for natural population multiplication like temperature and respectively. The weather factors noted optimum for spider population at Tandojam. It was concluded that the peak population of the pest was observed in different agro-eco-zones from September and October.