Population structure and antimicrobial resistance traits of avian‐origin mcr‐1 ‐positive Escherichia coli in Eastern China, 2015 to 2017

Abstract

Recent emergence of mcr-1-positive Escherichia coli (MCRPEC) is causing serious concern around the world. Due to poultry-origin E. coli holding zoonotic potential, the improved understandings of MCRPEC population structure and antimicrobial resistance are critical to public health purposes. This study provided novel insights into the molecular epidemiology of avian-origin MCRPEC. For the mcr genes prevalence study, we analysed 1,360 E. coli recovered from avian colibacillosis in eastern China from 2015 to 2017. The mcr-1 was present in 172 (12.6%) E. coli isolates.For all of MCRPEC isolates, MICs of colistin were ≥4 mg/L. Avian-origin MCRPEC was widely distributed throughout phylogroups A, B1, B2, D, and F. Moreover, those isolates were assigned to 52 unique STs, such as ST48, ST117, ST131, and ST648, suggesting substantial horizontal dissemination of mcr-1 gene through avian-origin E. coli populations. The susceptibility of MCRPEC isolates was tested with 26 antimicrobial agents from 16 antimicrobial categories. There were high resistance rates of MCRPEC isolates against the clinically used antibiotics. All MCRPEC isolates in this study presented the multidrug-resistant (MDR) trait were even considered as extensively drug-resistant (XDR) strains. Resistance genotypes and plasmid replicon profiling showed that a majority of MCRPEC isolates contained plasmid-mediated resistance genes and exhibited the co-existence of mcr-1 with ESBLs and pAmpCs genes. Furthermore, the overlapped distribution of ST types and resistance gene contents was detected among MCRPEC isolates from humans and poultry. Besides mcr-1, our findings highlighted a significant prevalence of plasmid-mediated resistance genes among avian-origin MCRPEC isolates. K E Y W O R D Santimicrobial susceptibility, avian-origin MCRPEC, resistance genotypes, sequence type

Prevalence and antimicrobial resistance of Salmonella isolated from broilers in Shandong, China

“…In this study, all the Salmonella resistance to PB, however, only 6.0 of the isolates carried mcr−1 which was different from the report that there was a close positive correlation between the resistance phenotypes and genotypes of the isolates [39].…”

Prevalence and Molecular Characteristics of Avian-Origin mcr-1-Harboring Escherichia coli in Shandong Province, China

“…In addition, the mcr-1 positive isolates showed higher resistance to PB than the mcr-1 negative E. coli isolates in this study, which suggested that there was a close positive correlation between the resistance phenotypes and genotypes of the mcr-1 positive isolates. This result is consistent with other reports (Huang et al, 2017;Shen et al, 2018;Zhuge et al, 2019).…”

“…In the current study, we noticed a high rate of resistance to AMP, AMO, and FLO in the mcr-1 positive isolates similar to a report in Eastern China from 2015 to 2017 (Zhuge et al, 2019), suggesting that these drugs may have been widely used in poultry farming. Most of the mcr-1 positive isolates showed susceptibility to AC, which was associated with the infrequent use of this drug, and the result was consistent with other reports (Dominguez et al, 2018).…”

Farm animals and aquaculture: significant reservoirs of mobile colistin resistance genes

“…However, levels of colistin resistance are consistently low. The gene mcr-1 has also been identified in bacteria from sheep (Tang et al, 2019), poultry (ducks, geese, and pigeons) (Zhang et al, 2018;Zhuge et al, 2019), companion animals (dogs and cats) (Sun et al, 2016;Zhang et al, 2016;Lei et al, 2017;Wang et al, 2018a;Ortega-Paredes et al, 2019;Rumi et al, 2019), and even wild animals (Liakopoulos et al, 2016;Mohsin et al, 2016;Ruzauskas and Vaskeviciute, 2016;Unger et al, 2017;Bachiri et al, 2018;Oteo et al, 2018) (Table 1 and Supporting Information Table S1). The first report of a wild animal carrying mcr-1 gene could be traced back to 2012, when Liakopoulos et al identified five mcr-1-positive E. coli from Larus dominicanus (n = 50) in Argentina, and all mcr-1 genes were located on IncI2 plasmids (Liakopoulos et al, 2016).…”