Population Structure, Antimicrobial Resistance, and Virulence-Associated Genes in Campylobacter jejuni Isolated From Three Ecological Niches: Gastroenteritis Patients, Broilers, and Wild Birds

Iglesias-Torrens, Yaidelys; Miró, Elisenda; Guirado, Pedro; Llovet, Teresa; Muñoz, Carmen; Cerdà‐Cuéllar, Marta; Madrid, Cristina; Balsalobre, Carlos; Navarro, Ferrán

doi:10.3389/fmicb.2018.01676

Cited by 39 publications

(45 citation statements)

References 61 publications

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“…Regarding the genes cdt coding for synthesis and deliver of the cytolethal distending toxin, our findings are similar with those reported by Bang et al [20], Datta et al [43] and Al-Mahmeed et al [44]. In our knowledge, activity of CDT toxin (consisting CDTA, CDTB and CDTC) lead to increase of intestinal epithelial cell volume by stopping cell division cycle at G2 step causing cell destruction [38,[45][46]. Indeed, after adhesion to fibronectin, C. jejuni colonizes intestinal cells and then invasion occurs simultaneously with production of CDT toxin causing inflammatorytype diarrhea in the host organism [47].…”

Section: Discussionsupporting

confidence: 91%

“…In addition, virulence genes cadF and cdtA were detected in all tested strains while, cdtB, and cdtC were isolated in 89.51% and 90.32% respectively in these isolates. This high prevalence of these four (4) genes isin agreement with several studies that found it in nearly all of theCampylobacterisolates [1,38]. The cadF gene is coding for a putative OmpA-like protein that mediates bacterial adhesion by binding to host fibronectin.…”

Section: Discussionsupporting

confidence: 90%

“…The high prevalence of this gene in our study could indicate this decisive role of the cadF gene in the virulence capacity of C. jejuni. However, Iglesias-Torrens et al [38] studies suggest that cadF may not be essential for the ability of Campylobacter to cause infection in humans. Therefore, we hypothesize that the high prevalence of the virulence factors in our finding is more indispensable for these strain in the colonization of the digestive tract of broilers chicken as reported by Ziprin et al [41] and Konkel et al [42].…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial resistance and virulence associated genes in Campylobacter jejuni isolated from chicken in Côte d’Ivoire

Goualié

Akpa

Kakou-Ngazoa

et al. 2019

J Infect Dev Ctries

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Introduction: Campylobacter jejuni is one of the major causes of gastroenteritis worldwide of the last century. The aim of this study was to investigate the antibiotics profiles and the virulence gene in C. jejuni strains isolated from chicken in Côte d’Ivoire. Methodology: A total of 336 chicken ceaca samples recovered from market of two municipality of Abidjan were examined by conventional microbiological methods and molecular test using PCR. The antibiotic susceptibility tests of the isolates were determined by disk diffusion method. The presence of virulence genes was examined using simple PCR method. Results: Among of 336 samples, 168 (50%) were positives for C. jejuni. Among the C. jejuni isolates, 159 strains (94.64%) were resistant to one or more antimicrobial agents. The highest percentage of antimicrobial resistance was found for Nalidixic acid (85.33%), Tétracyclin (71.76%) and Ciprofloxacin (55.65%). Moreover, MDR including 3, 4, 5 and 6 antibiotics families was detected in 16.66% of isolates. On the other hand, detection of virulence putative gene shows presence of cadF in 100% of tested strains. In addition, cdtA, cdtB and cdtC genes were detected in 100%; 89.51% and 90.32% respectively of C. jejuniisolates. Conclusion: Because of the key role of broiler chicken in human campylobacteriosis infection, it will important in first time to monitoring using of antibiotics in chicken farms and in second time to verify presence of campylobactériosis in country.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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Antimicrobial resistance and virulence associated genes in Campylobacter jejuni isolated from chicken in Côte d’Ivoire

Goualié

Akpa

Kakou-Ngazoa

et al. 2019

J Infect Dev Ctries

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“…The C. jejuni strain collection, previously described [15], is composed of 150 isolates obtained from faeces of three different sources: human patients suffering from symptomatic gastroenteritis (50 isolates), broiler chickens (50 isolates), and wild birds (50 isolates). Human isolates were obtained from the Santa Creu i Sant Pau Hospital (Barcelona) strain collection.…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

Differential Distribution of the wlaN and cgtB Genes, Associated with Guillain-Barré Syndrome, in Campylobacter jejuni Isolates from Humans, Broiler Chickens, and Wild Birds

et al. 2020

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Campylobacter jejuni causes campylobacteriosis, a bacterial gastroenteritis with high incidence worldwide. Moreover, C. jejuni infection can trigger the polyneuropathic disorder denominated Guillain-Barré syndrome (GBS). The C. jejuni strains that can elicit GBS carry either wlaN or cgtB, coding both genes for a β-1,3-galactosyltransferase enzyme that is required for the production of sialylated lipooligosaccharide (LOSSIAL). We described a differential prevalence of the genes wlaN and cgtB in C. jejuni isolates from three different ecological niches: humans, broiler chickens, and wild birds. The distribution of both genes, which is similar between broiler chicken and human isolates and distinct when compared to the wild bird isolates, suggests a host-dependent distribution. Moreover, the prevalence of the wlaN and cgtB genes seems to be restricted to some clonal complexes. Gene sequencing identified the presence of new variants of the G- homopolymeric tract within the wlaN gene. Furthermore, we detected two variants of a G rich region within the cgtB gene, suggesting that, similarly to wlaN, the G-tract in the cgtB gene mediates the phase variation control of cgtB expression. Caco-2 cell invasion assays indicate that there is no evident correlation between the production of LOSSIAL and the ability to invade eukaryotic cells.

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“…Both species have been associated with gastroenteritis in humans, therefore, it was expected the presence of putative virulence genes. On the contrary, it is possible that negative results for the other tested genes were due to differences in DNA sequences of those genes among strains because the primers have been designed on the basis of the representative strains C. jejuni 81-176 and A. butzleri RM4018 (Levican et al 2013, Iglesias-Torrens et al 2018. In this regard, Iglesias-Torrens et al (2018) have observed a higher diversity of cdtA alleles among C. jejuni strains found in wild birds compared to broilers or humans.…”

Section: Discussionmentioning

confidence: 99%

Faecal shedding of campylobacteria among domestic and wild animals from an urban coastal área

Levicán

Flores

Sánchez³

et al. 2019

Austral j. vet. sci.

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Campylobacter jejuni and Campylobacter coli are the leading cause of food-related diarrhea worldwide. However, other campylobacteria such as other Campylobacter spp., Arcobacter spp. and Helicobacter spp. are also recognised as emerging pathogens, although they are not frequently isolated by traditional culturing methods. Moreover, Campylobacter spp. have become increasingly resistant to antibiotics due to antibiotic usage in animal and human medicine. It has been suggested that pet ownership increases the risk for campylobacteriosis. However, environmental factors such as temperature, humidity, and poor sanitation conditions have also been associated with the highest rates of shedding among animals. This study assessed the faecal shedding of campylobacteria in an urban coastal area among 68 (66.0%) domestic animals, mainly dogs (n=61), and 35 (33.9%) wild animals belonging to 13 species and eight orders. None of them had symptoms of gastroenteritis and campylobacteria were detected by PCR in 21 samples (20.4%), while only six (5.8%) were detected by culturing, i.e. Campylobacter upsaliensis (n=4, dogs), C. jejuni (n=1, dog) and Arcobacter butzleri (n=1, chicken). None of the isolates was resistant to ciprofloxacin, but two were resistant to erythromycin, i.e. A. butzleri (MIC=8 µg/ml) and C. upsaliensis (MIC=128 µg/ml). Regarding the virulence factors, only one isolate of C. jejuni was positive for cdtC and cadF genes and one isolate of A. butzleri was positive for cadF and ciaB genes. This is the first study to assess the faecal shedding of campylobacteria in animals from the urban coastal area of Valparaíso, Chile. Although C. jejuni and other emerging campylobacteria were detected mainly from pet dogs and in a low rate, further investigations are needed to assess the potential transmission of these zoonotic bacteria or their antibiotic resistance between pets and owners.

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Population Structure, Antimicrobial Resistance, and Virulence-Associated Genes in Campylobacter jejuni Isolated From Three Ecological Niches: Gastroenteritis Patients, Broilers, and Wild Birds

Cited by 39 publications

References 61 publications

Antimicrobial resistance and virulence associated genes in Campylobacter jejuni isolated from chicken in Côte d’Ivoire

Antimicrobial resistance and virulence associated genes in Campylobacter jejuni isolated from chicken in Côte d’Ivoire

Differential Distribution of the wlaN and cgtB Genes, Associated with Guillain-Barré Syndrome, in Campylobacter jejuni Isolates from Humans, Broiler Chickens, and Wild Birds

Faecal shedding of campylobacteria among domestic and wild animals from an urban coastal área

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