Population Structure of Coimbatore Canes Developed in a Century of Sugarcane Breeding in India

Hemaprabha, G.; Pathy, T. Lakshmi; Mohanraj, K.; Alarmelu, S.; Ram, Bakshi

doi:10.1007/s12355-021-01093-0

Cited by 1 publication

(3 citation statements)

References 17 publications

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“…The sugarcane research community usually regarded Saccharum ( sensu stricto ) as containing six species, including two wild species ( S. spontaneum and S. robustum ), and four cultivated species - S. officinarum , S. edule , S. barberi , and S. sinense ( Purseglove, 1972 ; Daniels and Roach, 1987 ; Grivet et al., 2006 ; Hemaprabha et al., 2022 ). The present study, using both model-based clustering and multivariant analysis based on 357 single dose SNP makers, supported the current classification of Saccharum germplasm.…”

Section: Discussionmentioning

confidence: 99%

“…S. barberi and S. sinense are two cultivated species that are closely related ( Purseglove, 1972 ; Lu et al., 1994 ; Hemaprabha et al., 2022 ). However, the taxonomy status, as well as the relationship between these two species, has been a subject of debate.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, as a USDA National Plant Germplasm collection, the clones are freely distributed to international sugarcane community. From 2010 to 2021, a total of 9439 cuttings of various Saccharum spp., and 298 cuttings of 4 Tripidium spp., were distributed to researchers and breeders in the USA (44%) and internationally (56%) ( Hemaprabha et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Assessing the genetic integrity of sugarcane germplasm in the USDA-ARS National Plant Germplasm System collection using single-dose SNP markers

Park,

Zhang,

Ali

2024

Front. Plant Sci.

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The World Collection of Sugarcane and Related Grasses, maintained at the USDA-ARS in Miami, FL, is one of the largest sugarcane germplasm repositories in the world. However, the genetic integrity of the Saccharum spp. germplasm in this collection has not been fully analyzed. In this study, we employed a single-dose SNP panel to genotype 901 sugarcane accessions, representing six Saccharum species and various hybrids. Our analysis uncovered a high rate of clone mislabeling in the collection. Specifically, we identified 86 groups of duplicates, characterized by identical SNP genotypes, which encompassed 211 accessions (23% of the total clones), while 135 groups, constituting 471 clones (52% of the total), exhibited near-identical genotypes. In addition, twenty-seven homonymous groups were detected, which shared the same clone name but differed in SNP genotypes. Hierarchical analysis of population structure partitioned the Saccharum germplasm into five clusters, corresponding to S. barberi, S. sinense, S. officinarum, S. spontaneum and S. robustum/S. edule. An assignment test, based on the five Saccharum species, enabled correcting 141 instances of mislabeled species memberships and inaccuracies. Moreover, we clarified the species membership and parentage of 298 clones that had ambiguous passport records (e.g., ‘Saccharum spp’, ‘unknown’, and ‘hybrid’). Population structure and genetic diversity in these five species were further supported by Principal Coordinate Analysis and neighbor-joining clustering analysis. Analysis of Molecular Variance revealed that within-species genetic variations accounted for 85% of the total molecular variance, with the remaining 15% attributed to among-species genetic variations. The single-dose SNP markers developed in this study offer a robust tool for characterizing sugarcane germplasm worldwide. These findings have important implications for sugarcane genebank management, germplasm exchange, and crop genetic improvement.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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