Population Structure of Wild Musk Shrews (Suncus murinus) in Asia Based on Mitochondrial DNA Variation, with Research in Cambodia and Bhutan

Kurachi, Megumi; BA-Loc, Chau; Dang, Vu-Binh; Dorji, Tashi; Yamamoto, Yoshio; Nyunt, Maung Maung; Maeda, Yoshizane; Chhum-Phith, Loan; Namikawa, Takao; Yamagata, T.

doi:10.1007/s10528-006-9051-0

Cited by 12 publications

(23 citation statements)

References 28 publications

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“…Despite the monomorphic nature observed in this study, these 96 markers may be useful in population and other genetic studies of S. murinus, because successful PCR amplification was achieved in the three strains (TKU, BAN-kc,oeb and WZ). Their utility in these studies will complement work already done with mitochondria DNA [15,39,40], biochemical variants [33], morphometric parameters [11] and vitamin D binding protein [6]. All the 58 loci with clear alleles exhibited the co-dominant mode of inheritance.…”

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confidence: 76%

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A Genetic Linkage Map of the House Musk Shrew, Suncus murinus, Constructed with PCR-Based and RFLP Markers

et al. 2008

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Nagoya, Japan The establishment of a genetic map provides the foundation for the chromosomal localization of heritable characteristics, which ultimately may lead to the identification of genes underlying phenotypes of interest. With the advancement of molecular biology, new types of molecular genetic markers have been developed. These markers are classified into three types, type I markers (functional genes), type II markers (microsatellite or anonymous DNA sequences) and type III bi-allelic SNPs [22], and they have been used extensively to saturate the genetic maps of various species [23]. Before the present study, the genetic linkage map of the house musk shrew, Suncus murinus (Order Insectivora), comprised 11 loci including seven comparative anchor tagged sequences (CATS), and three microsatellite and one behavioral mutant genes [1,2]. In spite of the paucity of markers, S. murinus represents an important laboratory species for several reasons. Laboratory strains have been established from wild S. murinus trapped in different localities in Asia [7,8,21,24,25,32], and mutant stocks showing morphological and biochemical variants have been reported [9,10,[26][27][28]33]. Effects of various emetic and anti-emetic drugs have been studied in this species, because it is one of the few mammalian species that vomit in response to emetic drugs [36]

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confidence: 76%

“…The microsatellite markers developed in this study should be a valuable resource for molecular genetic studies, population genetics, and so on. [15].…”

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confidence: 99%

A Genetic Linkage Map of the House Musk Shrew, Suncus murinus, Constructed with PCR-Based and RFLP Markers

et al. 2008

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“…We speculate that the V240A variation developed after the separation of ancestral wild suncus into geographically distant regions. A phylogenetic tree deduced from the sequence of mitochondrial DNA control regions classifies KAT and RYU (OKI:Okinawa) into continental and insular groups [21], and indicates the substantial distance between KAT and RYU. The several variations in TYR identified between KAT and RYU strains, as well as the V240A variation in SLC45A2, may be used to elucidate how wild suncus segregated in different regions of Asia.…”

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confidence: 99%

Oculocutaneous Albinism in Suncus murinus: Establishment of a Strain and Identification of Its Responsible Gene

et al. 2009

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Abstract:The house musk shrew Suncus murinus (Insectivora, Soricidae) is referred to as suncus in a laboratory context. Although the capture of albino-like shrews (wild suncus) has been reported previously, albino-like strains have never been established, and the molecular basis of the character has remained elusive. We have established an OCAO mutant strain (oculocutaneous albinism Okinawa), from a wild suncus with a white coat and red eyes, which was captured in 2002. During the course of establishing the strain, it was revealed that the albino-like phenotype was inherited in an autosomal recessive manner. To elucidate the molecular basis of this phenotype, we cloned the suncus cDNAs for tyrosinase (Tyr), pinkeyed dilution (p), and solute carrier family 45, member 2 (Slc45a2), since these genes are involved in oculocutaneous albinism in various species, including humans. Several polymorphisms were identified in these genes; however, linkage analysis excluded the involvement of Tyr and p. On the other hand, two amino acid substitutions (V240A and G366E) were identified in Slc45a2 that cosegregated with the phenotype in the OCAO mutant strain. While V240A was also present in colored suncus collected from Okinawa, G366E was unique to the albino-like suncus and heterozygous carriers. Thus, we conclude that a mutation in Slc45a2 (G366E) is responsible for an albino-like phenotype in Suncus murinus.

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“…Previously, wild musk shrew populations in Asia, including those in Nepal, Bhutan, Bangladesh, Sri Lanka, Myanmar, Thailand, Malaysia, Indonesia, the Philippines, Vietnam, Cambodia, Japan, and Mauritius, were investigated based on mitochondrial DNA (mtDNA) restriction fragment length polymorphism (RFLP) analysis (Yamagata et al 1995(Yamagata et al , 1999(Yamagata et al , 2004Kurachi et al 2007). These studies revealed two largely different types of mtDNA, Continental and Island.…”

Section: Introductionmentioning

confidence: 99%

“…After an investigation of the Myanmar, Cambodia, and Bhutan populations, the possibility of partial migration routes was supported (Kurachi et al 2007). However, these routes are merely hypothetical, because the shrews in South India, which is the primary region, where the migration route and the evolutionary process of wild musk shrews can be confirmed, have not been investigated yet.…”

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confidence: 99%

Phylogeography of Wild Musk Shrew (Suncus Murinus) Populations in Asia Based on Blood Protein/Enzyme Variation

et al. 2007

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The musk shrew (Suncus murinus) is an insectivore species that inhabits tropical and subtropical Asia widely. To clarify the genetic relationship among wild musk shrew populations, we examined the electrophoretic variants of biparentally inherited genetic markers at 10 loci coding for eight blood proteins/enzymes in a total of 639 animals and compared the results obtained from the mitochondrial DNA data. The principal-component analysis performed using the allele frequency data revealed that the 17 populations could be divided into two major groups, a South Asian group and a Southeast Asian group that includes several island populations bound by Myanmar. The degrees of genetic divergence among populations were higher within the Southeast Asian group than within the South Asian group. This finding was incongruent with the mtDNA diversity. Analysis conducted at the individual level showed that a shrew from the central region in Myanmar that carries a South Asian type of mtDNA showed the electrophoretic variants specific to the Southeast Asian group, suggesting that this region is a contact zone between the two major groups.

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Population Structure of Wild Musk Shrews (Suncus murinus) in Asia Based on Mitochondrial DNA Variation, with Research in Cambodia and Bhutan

Cited by 12 publications

References 28 publications

A Genetic Linkage Map of the House Musk Shrew, Suncus murinus, Constructed with PCR-Based and RFLP Markers

A Genetic Linkage Map of the House Musk Shrew, Suncus murinus, Constructed with PCR-Based and RFLP Markers

Oculocutaneous Albinism in Suncus murinus: Establishment of a Strain and Identification of Its Responsible Gene

Phylogeography of Wild Musk Shrew (Suncus Murinus) Populations in Asia Based on Blood Protein/Enzyme Variation

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