Population variations in the Fungoid Frog Hylarana malabarica (Anura: Ranidae) from northern Western Ghats of India

Padhye, Anand; Jadhav, Anushree; Diwekar, Manawa; Dahanukar, Neelesh

doi:10.11609/jott.o2863.2343-52

Cited by 3 publications

(4 citation statements)

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“…This species was described by Tschudi (1838) from 'Malabar', India and subsequently reported from throughout the Western Ghats, central India (Dutta, 1997;Chanda, 2002;Daniels, 2005) and Meghalaya in Northeast India (Chanda, 2002). Padhye et al (2012) discussed genetic variability in six populations of Hylarana malabarica from Maharashtra in the Western Ghats and regarded them as a complex of species due to considerable morphological and genetic differences.…”

Section: Description Of Bnhs 5880 (Figsmentioning

confidence: 99%

DNA barcoding, phylogeny and systematics of Golden-backed frogs (Hylarana, Ranidae) of the Western Ghats-Sri Lanka biodiversity hotspot, with the description of seven new species

Biju

Garg

Mahony

et al. 2014

CTOZ

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A systematic revision of the genus Hylarana in the Western Ghats-Sri Lanka biodiversity hotspot is presented. Species delineation in Hylarana is complicated due to a lack of distinct colour differences or striking morphological characters, leading to potential misidentification. We conducted extensive surveys throughout the Western Ghats-Sri Lanka biodiversity hotspot and performed multiple gene (16S, COI and Cytb) barcoding using 103 samples collected from cultivated land and natural habitats. Genetic distance comparisons and Neighbor Joining trees indicated the presence of at least 14 candidate species in the region, supported by taxa groupings for all three genetic markers. Utilising a combination of molecular and morphological data, we describe seven new species, doubling the number of Hylarana species previously known from this region. We further demonstrate that H. temporalis, which was originally described from Sri Lanka, was misidentified with the Western Ghats endemic species for nearly 100 years. Conversely, H. aurantiaca was originally described from the Western Ghats and misidentified in Sri Lanka. Our study confirms that the distribution of H. temporalis is restricted to Sri Lanka, while H. aurantiaca is endemic to the Western Ghats, and that there are no shared Hylarana species between the two regions. Hylarana flavescens, H. intermedius and H. montanus, previously considered synonyms of H. temporalis are confirmed as valid species. Hylarana bhagmandlensis is removed from the synonymy of H. aurantiaca and placed as a junior subjective synonym of H. montanus. To establish nomenclatural stability, H. flavescens, H. malabarica and H. temporalis are lectotypified and H. intermedius is neotypified. Detailed descriptions, diagnosis, morphological and genetic comparisons, illustrations and data on distribution and natural history are provided for all species. Phylogenetic analyses based on three mitochondrial markers (16S, COI and Cytb) and a fragment of the nuclear Rag1 gene, show complete endemism of the Western Ghats-Sri Lankan species. Four major groups in this region are identified as: 1 — the Hylarana aurantiaca group, endemic to the Western Ghats; 2 — the Hylarana flavescens group, endemic to the Western Ghats; 3 — the Hylarana temporalis group, endemic to Sri Lanka; and 4 — the Hylarana malabarica group from Sri Lanka and India. The discovery of numerous morphologically cryptic Hylarana species in this region further emphasizes the benefits of utilizing an integrative taxonomic approach for uncovering hidden diversity and highlighting local endemism in the Western Ghats-Sri Lanka biodiversity hotspot.

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Section: Description Of Bnhs 5880 (Figsmentioning

confidence: 99%

DNA barcoding, phylogeny and systematics of Golden-backed frogs (Hylarana, Ranidae) of the Western Ghats-Sri Lanka biodiversity hotspot, with the description of seven new species

Biju

Garg

Mahony

et al. 2014

CTOZ

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“…All the six populations of H. malabaricus studied by Padhye et al (2012) should now be attributed to the new species H. bahuvistara based on the genetic analysis presented in the current study. Padhye et al (2012) showed that within the six populations of H. bahuvistara there are morphological as well as genetic variations in Randomly Amplified Polymorphic DNA markers. In the current study, however, we show that there is little genetic distance in the 16S rRNA gene among the populations of H. bahuvistara.…”

Section: Discussionmentioning

confidence: 65%

“…In the current study, however, we show that there is little genetic distance in the 16S rRNA gene among the populations of H. bahuvistara. Nevertheless, it is also true that the populations of H. bahuvistara are highly fragmented because of habitat fragmentation and therefore, the population variation reported by Padhye et al (2012) could be due to recent fragmentation of populations of H. bahuvistara.…”

Section: Discussionmentioning

confidence: 99%

Hydrophylax bahuvistara, a new species of fungoid frog (Amphibia: Ranidae) from peninsular India

et al. 2015

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Funding sources had no role in study design, data collection, results interpretation and manuscript writing.For Author Contribution and Author Details see end of this article. is also thankful to the staff at MNHN for their kind help. We are thankful to Joseph Martinez, Herpetology section, Museum of Comparative Zoology, Harvard University, for photographs of Hydrophylax leptoglossa syntypes. We are also thankful to Bartosz Borczyk, Natural History Museum, Wroclaw University, for helping ADP and NM with examination of a specimen of Hydrophylax gracilis. We also thank Dr. P.S. Bhatnagar, officer-in-charge, and Dr. Shrikant Jadhav, Zoological Survey of India, Western Regional Center (ZSI-WRC), Pune, for their help in registering specimens in ZSI-WRC. We are thankful to Dr. Sanjay Molur and Keerthi Krutha for helping in registration of specimens in the Wildlife Information Liaison Development (WILD), Coimbatore. We are also thankful to

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“…Snell & Evans (2006) used RAPD to differentiate the larva of Rana arvialis and Rana temporaria that were morphologically similar. Padhye et al (2012) reported variations on the population of Hylarana malabarica in northern West Ghat, India based on RAPD markers. Therefore, this study aimed to look at the genetic polymorphism and determine the locus diversity of R. margaritifer population in Baturraden using the PCR-RAPD technique.…”

Section: Introductionmentioning

confidence: 99%

Diversity analysis of <i>Rhacophorus margaritifer</i> (Schlegel, 1837) in Baturraden based on RAPD markers

Sulistyahadi

Puspitasari

Nuryanto

2020

J.Tropical Biodiversity Biotechnology

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Rhacophorus margaritifer is an endemic species of arboreal frog in Java. Previous studies found this frog in several locations of Baturraden, namely in primary forest of Ketenger area, along Pancuran Pitu tourism track, and Baturraden Botanical Garden. There were still limited studies of molecular diversity of R. margaritifer and no prior data from population in the southern slope of Mount Slamet. This study aimed to look at the genetic polymorphism and determine the locus diversity of R.margaritifer population in Baturraden by using the PCR-RAPD technique. Frog tissue samples were taken from three populations in Kalipagu hiking trail (HPK 01), Baturraden hiking trail (HPK 02), and Baturraden Botanical Garden (KRB). DNA was extracted using the Chelex method. Molecular characterization was performed based on RAPD markers. The RAPD marker band pattern was changed to binary data 0-1 and analysed using Arlequin software ver.3.5. A total of 19 frog individuals were obtained during sampling at three locations. High genetic diversity had been observed in all populations with gene diversity range from 0.9643 in HPK 01 population to 1.0000 in both KRB and HPK 02 populations. A high locus variation was also observed for all populations with values of 0.159524 in KRB; 0.165816 in HPK 01; 0.192857 in HPK 02, respectively. AMOVA indicated no genetic difference among populations of R. margaritifer (p=0.50244).

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Population variations in the Fungoid Frog Hylarana malabarica (Anura: Ranidae) from northern Western Ghats of India

Cited by 3 publications

References 16 publications

DNA barcoding, phylogeny and systematics of Golden-backed frogs (Hylarana, Ranidae) of the Western Ghats-Sri Lanka biodiversity hotspot, with the description of seven new species

DNA barcoding, phylogeny and systematics of Golden-backed frogs (Hylarana, Ranidae) of the Western Ghats-Sri Lanka biodiversity hotspot, with the description of seven new species

Hydrophylax bahuvistara, a new species of fungoid frog (Amphibia: Ranidae) from peninsular India

Diversity analysis of <i>Rhacophorus margaritifer</i> (Schlegel, 1837) in Baturraden based on RAPD markers

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