Populations of Armillaria species in pine plantations in west-central Poland

Szewczyk, Wojciech; Kwaśna, Hanna; Behnke‐Borowczyk, Jolanta

doi:10.12657/denbio.074.010

Cited by 2 publications

(4 citation statements)

References 67 publications

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“…A similar number of wood-inhabiting species ( 58), compared to our study (57), in spruce stands on former agricultural land was found in a 3-year study in Slovakia in spruce stands aged 21-51 years [64]. It is worth noting that in the cited studies, typical spruce pathogens, such as Armillaria spp.…”

Section: Discussionsupporting

confidence: 87%

“…Identification of Heterobasidion specimens was performed by speciesspecific PCR with the primer pairs MJ-F/MJ-R (H. annosum) and KJ-F/KJ-R (H. parviporum) [56]. Identification of Armillaria species was performed based on sequencing of a portion of the translation elongation factor 1 alpha (tef 1-alpha, amplified according to Szewczyk et al (2015) [57]. The amplified regions had a size of 100 bp (H. annosum), 350 bp (H. parviporum), and 600-650 bp (tef 1-alpha).…”

Section: Preparation Of Pure Cultures and Molecular Analysesmentioning

confidence: 99%

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Fungi Occurring in Norway Spruce Wood Decayed by Heterobasidion parviporum in Puszcza Borecka Stands (Northeastern Poland)

Szczepkowski

Kowalczuk²,

Sikora

et al. 2022

Forests

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In many spruce stands, trees are frequently attacked by the pathogen Heterobasidion parviporum, albeit without visible symptoms in the crown. In the present work, the results of the presence of stem rot, assessed by PICUS Sonic Tomography, and the fungal biota on trees and stumps in eight plots in the Puszcza Borecka Forest are described. The plots were located in stands on original forest soil (4) and on post-agricultural soil (4), where around a stump with H. parviporum symptoms (signs of internal rot and basidiocarps), 30 trees were selected and examined for internal rot. Wood samples were collected from two selected trees for fungal molecular analysis. A total of 79 fungal taxa were found, including 57 taxa in plots on post-agricultural soil and 45 on forest soil. There were 395 fungal records on stumps and 22 records on trees, therein, from the inner parts of felled trunks. Significant differences in the Chao-1 diversity index indicate that the origin of the soil—post-agricultural or forest soil—influenced the alpha diversity of the fungal communities in the forests studied. The values of the Shannon and Simpson indices show that the two communities were similar in terms of species numbers. The presence of basidiomata of H. parviporum and two species of Armillaria (mainly A. cepistipes) in samples on all plots is striking, although Armillaria spp. was detected more frequently. Most of the species identified were typical saprotrophs, although rare species were also found, such as Entoloma byssisedum, Onnia tomentosa, Physisporinus vitreus, Postia ptychogaster, and Ramaria apiculata. The presence of H. parviporum in the inner woody parts was confirmed by PCR analysis, and decay was detected even up to a stem height of 6 m. Armillaria was the dominant genus in the studied stands and plays a significant and underestimated role in heartwood decay of old spruce trees in Puszcza Borecka Forest.

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Section: Discussionsupporting

confidence: 87%

Section: Preparation Of Pure Cultures and Molecular Analysesmentioning

confidence: 99%

Fungi Occurring in Norway Spruce Wood Decayed by Heterobasidion parviporum in Puszcza Borecka Stands (Northeastern Poland)

Szczepkowski

Kowalczuk²,

Sikora

et al. 2022

Forests

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“…An earlier acceptance of variation within VCGs of both Armillaria species is therefore here qualified (Hood 2012;Hood et al 2019). However, this does not mean that such within-VCG variation does not occur with Armillaria species (Kile 1983;Guillaumin et al 1996;Szewczyk et al 2015), just that it appears to be less likely among isolates taken from the same individual vegetative field colonies. In the present study a difference was apparent between subcultures of two isolates (Alim_33_31.1 and Alim_33_34.1) and those of the other isolates within VCG 33 (A. limonea).…”

Section: Discussionmentioning

confidence: 99%

“…Identifying and plotting VCGs has been helpful in studying Armillaria communities in both natural vegetation and artificial ecosystems in different parts of the world (e.g., Shaw & Roth 1976;Korhonen 1978;Kile 1983Kile , 1986Hood & Morrison 1984;Rishbeth 1991;Rizzo et al 1995;Guillaumin et al 1996;Legrand et al 1996;Abomo-Ndongo & Guillaumin 1997;Bruhn et al 1997;Dettman & van der Kamp 2001;Ferguson et al 2003;Prospero et al 2003;Mihail & Bruhn 2005;Szewczyk et al 2015;and others). Similar work in New Zealand has shown that Armillaria novae-zelandiae (G. Stevenson) Herink and Armillaria limonea (G. Stevenson) Boesewinkel occur in the form of numerous VCGs in native forests, plantations and orchards of kiwifruit (Actinidia deliciosa (A.Chev.)…”

Section: Introductionmentioning

confidence: 99%

Differentiating individuals of Armillaria species in New Zealand forests

Dodd¹,

Shah

Kimberley³

et al. 2022

NZJFS

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Background: Armillaria novae-zelandiae and A. limonea occur naturally as wood decay fungi in native forests in New Zealand. As pathogens they are responsible for significant root disease in trees and shrubs in plantations, crops and urban parks and gardens. A thorough understanding of their population dynamics entails knowledge of the spatial arrangement of their individual mycelia or genets. In previous work the distributions of vegetative compatibility groups (VCGs) of these fungi were mapped in an area of native forest prior to and after replacement by a young Pinus radiata plantation. With the advent of molecular technology, it has become possible to test species identities made earlier using culture techniques and to verify whether or not their VCGs, determined by incompatibility reactions between paired cultures, represent distinct individual genets. Methods: Stock subcultures of isolates representing each VCG were recovered from storage in order to obtain DNA. Extracted DNA was subjected to a polymerase chain reaction procedure (UP-PCR) using 11 universal primers to assess genetic variation between subcultures. Bands were scored as either present or absent for each primer-subculture combination and cluster analysis was undertaken by generating dendrogram trees to reveal genetic groupings among subcultures. Results: DNA cluster analysis divided subcultures of isolates into two species groups, A. novae-zelandiae and A. limonea, corresponding to identities determined through culture morphology. Within species, subcultures grouped into clusters that matched VCGs determined by earlier culture pairing. There was little indication of genetic variation within VCGs, except for one of A. limonea, which comprised two sub-clusters. Conclusions: The Armillaria species and VCGs identified by culture techniques in the laboratory were verified by independent molecular methodology. In general, the VCGs represent discrete individual genets or colonies in the field. Techniques that differentiate isolates based on differences in their DNA sequence provide a quick alternative to time-consuming laboratory culture methods for resolving population spatial structure. However, some complementary isolate pairing may be necessary when rationalising the significance of groupings in dendrogram trees.

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Populations of Armillaria species in pine plantations in west-central Poland

Cited by 2 publications

References 67 publications

Fungi Occurring in Norway Spruce Wood Decayed by Heterobasidion parviporum in Puszcza Borecka Stands (Northeastern Poland)

Fungi Occurring in Norway Spruce Wood Decayed by Heterobasidion parviporum in Puszcza Borecka Stands (Northeastern Poland)

Differentiating individuals of Armillaria species in New Zealand forests

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