Porcine Circovirus 2 Inclusion Bodies in Pulmonary and Renal Epithelial Cells

Huang, Yanyun; Walther, I.; Martinson, Shannon A.; Lopez, Alfonso; Yason, Carmencita V.; Godson, Dale L.; Clark, Edward G.; Simko, Elemir

doi:10.1354/vp.45-5-640

Cited by 15 publications

(10 citation statements)

References 10 publications

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“…At present, some works about PCV-2 inclusion bodies in lymph nodes, lungs and kidneys in PMWS have been achieved in a few studies Huang et al 2008). These studies have shown the intracytoplasmic inclusions in macrophages in lymphoid organs, bronchial glandular and renal tubular epithelial cells of PCV-2 infected pig.…”

Section: Discussionmentioning

confidence: 99%

Viral distribution and lesions in Kunming mice experimentally infected with porcine circovirus type 2b

Deng

Wang

et al. 2011

Vet Res Commun

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The viral distribution and lesions in Kunming mice experimentally infected with porcine circovirus type 2b (PCV-2b) were investigated. Seventy special pathogen free mice were divided into 2 groups with 35 mice in each group. The test group (TG) was infected with PCV-2b, the control group (CG) was inoculated with sterile cell cultures. Five mice in each group were sacrificed at 3, 7, 14, 21, 28, 35 and 42 dpi (day post infection), respectively. Necropsies were performed on all mice and tissues were collected for testing by histopathology, immunohistochemistry, transmission electron microscope (TEM) and polymerase chain reaction (PCR). Apoptosis and necrosis in lymphoid organs were observed in virus-infected mice, and became severe from 14 to 28 dpi. The proportion of PCV-2b antigen-positive cells was moderate in lung, heart, thymus, liver or kidney, and low in brain from TG. In spleen and cervical lymph node, the proportions of PCV-2b antigen-positive cells were low to high from 7 to 28 dpi, and moderate from 35 to 42 dpi. PCV-2b DNA was detected in all tissues examined in TG from 7 to 42 dpi. Viral inclusion bodies presented in the cytoplasm of lymphocytes, macrophages, hepatocytes, podocytes, neurocytes, spermatids and uterine epithelial cells in TG. In CG, no viruses and viral lesions were detected. PCV-2b could replicate in mice, and PCV-2b associated lesions in mice were similar to those observed in pigs. The present results indicate that it is possible to use Kunming mouse as an animal model for PMWS research.

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Section: Discussionmentioning

confidence: 99%

Viral distribution and lesions in Kunming mice experimentally infected with porcine circovirus type 2b

Deng

Wang

et al. 2011

Vet Res Commun

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“…44 Intracytoplasmic botryoid inclusion bodies can rarely be observed in the renal tubular epithelium in pigs with systemic PCVAD. 38 Renal tubular epithelial necrosis with extensive interstitial edema and hemorrhage; lymphoplasmacytic, eosinophilic, and histiocytic tubulointerstitial nephritis; and numerous botryoid intracytoplasmic inclusions within the renal tubular epithelium and interstitial macrophages were identified in a pig with acute renal failure. These lesions were associated with PCV2 antigen by IHC and PCV2 virus particles by electron microscopy indicating a causative relationship.…”

Section: Urinary Systemmentioning

confidence: 94%

“…23 Recently, it was reported that intracytoplasmic botryoid inclusion bodies that displayed PCV2-specific staining were observed in bronchial and bronchial glandular epithelial cells. 38 Mild nonsuppurative interstitial pneumonia 51,82,88 or broncho-pneumonia, 10,123 perhaps due to concurrent bacterial infection, have been reported in fetuses and newborn piglets infected with PCV2 in utero. PCV2 antigen was also associated with intraluminal polyps in bronchiolitis obliterans organizing pneumonia.…”

Section: Respiratory Systemmentioning

confidence: 99%

Current State of Knowledge on Porcine Circovirus Type 2-Associated Lesions

Opriessnig

Langohr

2012

Vet Pathol

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Porcine circovirus type 2 (PCV2), a small single-stranded DNA virus, was initially discovered in 1998 and is highly prevalent in the domestic pig population. Disease manifestations associated with PCV2 include postweaning multisystemic wasting disease (PMWS), enteric disease, respiratory disease, porcine dermatitis and nephropathy syndrome (PDNS), and reproductive failure. Although these clinical manifestations involve different organ systems, there is considerable overlap in clinical expression of disease and presence of lesions between pigs and within herds. It is now widely accepted that PCV2 can be further subdivided into different types, of which PCV2a and PCV2b are present worldwide and of greatest importance. This review will focus on PCV2-associated lesions in different organ systems.

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“…Meanwhile, the presence of typical granulomatous lesions with multinuclear giant cells was also recorded in the lymphatic tissue (Becskei et al, 2010). PCV2 inclusion bodies were demonstrated to be located in the cytoplasm of epithelial cells by immunohistochemical staining for PCV2 and cytokeratin antigens and by ultrastructural demonstration of viral particles in the inclusion bodies within renal tubular epithelium (Huang et al, 2008).…”

mentioning

confidence: 91%

Correlation of viral load with lesion severity in field pigs affected with porcine circovirus type 2

Katsuda¹,

Li²,

Kawashima³

2016

Afr. J. Agric. Res.

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Porcine circovirus type 2 (PCV2) is an important infection factor causing post weaning multisystemic wasting syndrome (PMWS). The amount of PCV2 viral load may mainly give rise to clinical symptom and pathological lesion of PMWS. In order to investigate the relationship between PCV2 viral load and the lesion severity of lymph tissues, and search further for some clues of pathologic diagnosis of PMWS, thirty pig cases affected with PCV2 and aged 30～90 days old were collected from swine farms and their lymph tissues were treated by quantitative Real Time PCR, immunohistochemistry (IHC) and histopathology examination. Four various groups were classified according to their evaluation scores for lesion severity, and it is shown that the higher the score for pathological lesion of lymph tissues, the more there is viral load in tonsil. Especially, the amount of PCV2 DNA in group one was 1/1000 lower than other three groups. It is supposed that group one is considered as subclinical case and the other three groups as clinical PMWS cases. Furthermore, it is likely presumed whether PCV2 infection is subclinical or clinical PMWS case can be helpfully diagnosed by these criteria.

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Porcine Circovirus 2 Inclusion Bodies in Pulmonary and Renal Epithelial Cells

Cited by 15 publications

References 10 publications

Viral distribution and lesions in Kunming mice experimentally infected with porcine circovirus type 2b

Viral distribution and lesions in Kunming mice experimentally infected with porcine circovirus type 2b

Current State of Knowledge on Porcine Circovirus Type 2-Associated Lesions

Correlation of viral load with lesion severity in field pigs affected with porcine circovirus type 2

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