Porcine circovirus type II screening in feral swine population in Ukraine

Rudova, Nataliia; Болотін, В. І.; Solodiankin, Oleksii; Gerilovych, Anton

doi:10.36016/jvmbbs-2019-5-3-2

Cited by 2 publications

(10 citation statements)

References 7 publications

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“…NCBI GenBank, accessed 27 April 2022, available at: ) ( Table S3 ). We applied a PCR diagnostics assay that we had previously developed [ 23 ] to examine archived blood samples from wild boar that had been opportunistically hunted across Ukraine in 2012, and found a PCV2 prevalence of 31.8% ( n = 34 of 107 samples). We also collected and identified PCV2 in a sampling of domestic pork products (liver) from a market in Kharkiv in 2019 (37% prevalence).…”

Section: Discussionmentioning

confidence: 99%

“…Primers targeting ORF1 were forward primer PCV-2F 5’-GAAGACGAGCGCAAGAAAATACG-3’, and reverse primer PCV-2R 5’-CCAATCACGCTTCTGCATTTTCCC-3’. The diagnostic primers flank a 421 nt variable region of the tail Rep gene [ 23 ]. For each sample, 5 μL of the eluate was run in a 25 μL reaction, with an annealing step at 60 °C, on a conventional PCR machine (Biometra TAdvanced Thermal Cycler, Analytik Jena GmbH).…”

Section: Methodsmentioning

confidence: 99%

“…Positive swine DNA samples were used to generate of ORF2 DNA amplicons (798 bp) by PCR for sequencing, using primers reported previously [ 23 ] Rudova et al, 2019). Briefly, PCR for ORF2 sequencing was performed using conventional PCR and Platinum SuperFi II Taq DNA Polymerase (ThermoFischer/Invitrogen, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Ukraine is a major pork producer and harbors both large domestic swine (commercial and backyard) and wild boar populations. These swine populations have been subjected to PCV2 infection [ 23 ] and swine influenza [ 24 ] in wild boar, and porcine teschovirus 1 [ 25 ] and ASF in both wild and domestic animals [ 26 , 27 ]. The circulation of PCV2 genotypes in swine in Ukraine is poorly understood and suffers from the sporadic nature of surveillance.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity of Porcine Circovirus 2 in Wild Boar and Domestic Pigs in Ukraine

Rudova

Buttler

Kovalenko

et al. 2022

Viruses

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Porcine circovirus type 2 (PCV2) is responsible for a number of porcine circovirus-associated diseases (PCVAD) that can severely impact domestic pig herds. For a non-enveloped virus with a small genome (1.7 kb ssDNA), PCV2 is remarkably diverse, with eight genotypes (a–h). New genotypes of PCV2 can spread through the migration of wild boar, which are thought to infect domestic pigs and spread further through the domestic pig trade. Despite a large swine population, the diversity of PCV2 genotypes in Ukraine has been under-sampled, with few PCV2 genome sequences reported in the past decade. To gain a deeper understanding of PCV2 genotype diversity in Ukraine, samples of blood serum were collected from wild boars (n = 107) that were hunted in Ukraine during the November–December 2012 hunting season. We found 34/107 (31.8%) prevalence of PCV2 by diagnostic PCR. For domestic pigs, liver samples (n = 16) were collected from a commercial market near Kharkiv in 2019, of which 6 out of 16 (37%) samples were positive for PCV2. We sequenced the genotyping locus ORF2, a gene encoding the PCV2 viral capsid (Cap), for 11 wild boar and six domestic pig samples in Ukraine using an Oxford Nanopore MinION device. Of 17 samples with resolved genotypes, the PCV2 genotype b was the most common in wild boar samples (10 out of 11, 91%), while the domestic pigs were infected with genotypes b and d. We also detected genotype b/d and b/a co-infections in wild boars and domestic pigs, respectively, and for the first time in Ukraine we detected genotype f in a wild boar from Poltava. Building a maximum-likelihood phylogeny, we identified a sublineage of PCV2 genotype b infections in both wild and domestic swine, suggesting a possible epizootic cluster and an ecological interaction between wild boar and domestic pig populations in northeastern Ukraine.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic Diversity of Porcine Circovirus 2 in Wild Boar and Domestic Pigs in Ukraine

Rudova

Buttler

Kovalenko

et al. 2022

Viruses

View full text Add to dashboard Cite

show abstract

“…Ukraine is a major pork producer, and harbors both large domestic swine (commercial and backyard) and wild boar populations. These swine populations have been subjected to PCV2 infection [23] and swine influenza [24] in wild boars, and porcine teschovirus 1 [25] and ASF in both wild and domestic animals [26,27]. The circulation of PCV2 subtypes in swine in Ukraine is poorly understood and suffers from the sporadic nature of surveillance.…”

Section: Pcv2 Genome and Subtypesmentioning

confidence: 99%

Genetic Diversity of Porcine Circovirus 2 Subtypes in Wild Boar and Domestic Pigs in Ukraine

Rudova¹,

Buttler²,

Kovalenko³

et al. 2022

Preprint

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Porcine circovirus type 2 (PCV2) is responsible for a number of porcine circovirus associated diseases (PCAD) that can severely impact domestic pig herds. For a non-enveloped virus with a small genome (1.7 kb ssDNA), PCV2 is remarkably diverse, with 8 subtypes (a-h). New subtypes of PCV2 can spread through migration of wild boars, which are thought to infect domestic pigs and spread further through the domestic pig trade. Despite a large swine population, the diversity of PCV2 subtypes in Ukraine has been undersampled, with few PCV2 genome sequences reported in the past decade. To gain a deeper understanding of PCV2 subtype diversity in Ukraine, samples of blood serum were collected from wild boars (n = 107) that were hunted in Ukraine during the November-December 2012 hunting season. We found 34/107 (31.8%) prevalence of PCV2 by diagnostic PCR. For domestic pigs, liver samples (n = 16) were collected from a commercial market near Kharkiv in 2019, of which 6/16 (37%) were positive for PCV2. We sequenced the genotyping locus ORF2, a gene encoding the PCV2 viral capsid (Cp), for 11 wild boar and 6 domestic pig samples in Ukraine using an Oxford Nanopore MinION device. Of 17 samples with resolved subtypes, PCV2 subtype b was most common in wild boar (10/11, 91%), while domestic pigs were infected with subtypes b and d. We also detected subtype b/d and b/a co-infections in wild boar and domestic pigs, respectively, and subtype f in a wild boar from Poltava for the first time in Ukraine. Building a maximum likelihood phylogeny, we identified a sublineage of PCV2 subtype b infections in both wild and domestic swine, suggesting a possible epizootic cluster and ecological interaction in northeastern Ukraine.

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Porcine circovirus type II screening in feral swine population in Ukraine

Cited by 2 publications

References 7 publications

Genetic Diversity of Porcine Circovirus 2 in Wild Boar and Domestic Pigs in Ukraine

Genetic Diversity of Porcine Circovirus 2 in Wild Boar and Domestic Pigs in Ukraine

Genetic Diversity of Porcine Circovirus 2 Subtypes in Wild Boar and Domestic Pigs in Ukraine

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