Porcine circoviruses—Small but powerful

Finsterbusch, Tim; Mankertz, Annette

doi:10.1016/j.virusres.2009.02.009

Cited by 183 publications

(131 citation statements)

References 57 publications

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“…This group of viruses includes the circoviruses. Porcine circovirus type 2 has been linked to postweaning multisystemic wasting disease in pigs, and disease onset and symptom severity are influenced by intrinsic factors, such as immune system status and genetic predisposition (51). No circoviruses have yet been linked to human diseases.…”

Section: Discussionmentioning

confidence: 99%

Intestinal virome changes precede autoimmunity in type I diabetes-susceptible children

Zhao

Vatanen

Droit

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

254

240

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Viruses have long been considered potential triggers of autoimmune diseases. Here we defined the intestinal virome from birth to the development of autoimmunity in children at risk for type 1 diabetes (T1D). A total of 220 virus-enriched preparations from serially collected fecal samples from 11 children (cases) who developed serum autoantibodies associated with T1D (of whom five developed clinical T1D) were compared with samples from controls. Intestinal viromes of case subjects were less diverse than those of controls. Among eukaryotic viruses, we identified significant enrichment ofCircoviridae-related sequences in samples from controls in comparison with cases. Enterovirus, kobuvirus, parechovirus, parvovirus, and rotavirus sequences were frequently detected but were not associated with autoimmunity. For bacteriophages, we found higher Shannon diversity and richness in controls compared with cases and observed that changes in the intestinal virome over time differed between cases and controls. Using Random Forests analysis, we identified disease-associated viral bacteriophage contigs after subtraction of age-associated contigs. These disease-associated contigs were statistically linked to specific components of the bacterial microbiome. Thus, changes in the intestinal virome preceded autoimmunity in this cohort. Specific components of the virome were both directly and inversely associated with the development of human autoimmune disease.

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Section: Discussionmentioning

confidence: 99%

Intestinal virome changes precede autoimmunity in type I diabetes-susceptible children

Zhao

Vatanen

Droit

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

254

240

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“…Porcine circovirus (PCV) is a small nonenveloped virus with a single-stranded circular DNA genome of approximately 1.7 kb in the family Circoviridae (11,28). Type 1 PCV (PCV1) was discovered as a contaminant of PK-15 cells and is nonpathogenic in pigs (26,27).…”

mentioning

confidence: 99%

Chimeric Porcine Circoviruses (PCV) Containing Amino Acid Epitope Tags in the C Terminus of the Capsid Gene Are Infectious and Elicit both Anti-Epitope Tag Antibodies and Anti-PCV Type 2 Neutralizing Antibodies in Pigs

Beach

Smith

Ramamoorthy

et al. 2011

J Virol

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A chimeric porcine circovirus (PCV1-2) with the capsid gene of pathogenic PCV2 cloned into the genomic backbone of nonpathogenic PCV1 is attenuated in pigs but elicits protective immunity against PCV2. In this study, short epitope tags were inserted into the C terminus of the capsid protein of the chimeric PCV1-2 vaccine virus, resulting in a tractable marker virus that is infectious both in vitro and in vivo. Pigs experimentally infected with the epitope-tagged PCV1-2 vaccine viruses produced tag-specific antibodies, as well as anti-PCV2 neutralizing antibodies, indicating that the epitope-tagged viruses could potentially serve as a positive-marker modified live-attenuated vaccine.

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“…ORF2 encodes proteins that are associated with viral structure and virulence, such as Cap (Horlen et al, 2008). The Cap protein is considered one of the major antigenic determinants, and functions efficiently in viral attachment to host cell surface receptors (Misinzo et al, 2006;Finsterbusch and Mankertz, 2009). Guo et al (2011) reported that recombinant viruses obtained higher replication with antigenic drift.…”

Section: Discussionmentioning

confidence: 99%

Identification of natural recombinants derived from PCV2a and PCV2b

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Porcine circovirus type 2 (PCV2) is considered to be the main pathogen in PC-associated diseases, and significantly affects the global pig-producing industry. PCV2 continuously evolves by point mutations and genome recombinations. In the present study, we aimed to further identify recombinant PCV2 strains. We used polymerase chain reaction to detect PCV2 in the carcasses of pigs with suspected infections from different regions of Guangdong Province in China. DNA was extracted from samples with confirmed infection and fullgenome amplification, sequencing, phylogenetic tree construction, gene recombination detection, and sequence alignment were performed in gene recombination analysis. Our results show that recombination occurred between the strains SHC (DQ104421) and ZhuJi2003 (AY579893). The recombination resulted in three recombinants: GD003 (KM503044), GD005 (KM487708), and GD008 (KM487709). Further analyses revealed that these novel recombinants appeared to result from recombination between the PCV2a and PCV2b strains, with crossover regions located in ORF2. This study was a comprehensive analysis that used several different methods, which demonstrated that a cluster of PCV2 strains resulted from the same type of inter-genotypic 11781Recombinants from PCV2 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 11780-11790 (2015) recombination pattern, with a breakpoint in the structural protein coding region. The results of our study provide both information on the recombination mechanism and disease pathogenesis and useful data for the prevention of PCV2 in the swine industry.

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Porcine circoviruses—Small but powerful

Cited by 183 publications

References 57 publications

Intestinal virome changes precede autoimmunity in type I diabetes-susceptible children

Intestinal virome changes precede autoimmunity in type I diabetes-susceptible children

Chimeric Porcine Circoviruses (PCV) Containing Amino Acid Epitope Tags in the C Terminus of the Capsid Gene Are Infectious and Elicit both Anti-Epitope Tag Antibodies and Anti-PCV Type 2 Neutralizing Antibodies in Pigs

Identification of natural recombinants derived from PCV2a and PCV2b

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